In this paper, we demonstrate high-resolution, multimodal in vivo imaging of human skin using optical coherence (OCM) and multiphoton microscopy (MPM). These two modalities are integrated into a single instrument to enable simultaneous acquisition and coregistration. The system design and the OCM image processing architecture enable sufficient performance of both modalities for in vivo imaging of human skin. Examples of multimodal in vivo imaging are presented as well as time lapse imaging of blood flow in single capillary loops. By making use of multiple intrinsic contrast mechanisms this integrated technique improves the ability to noninvasively visualize living tissue. Integrated OCM and MPM has potential applications for in vivo diagnosis of various pathological skin conditions, such as skin cancer, as well as potential pharmaceutical and cosmetic research applications.

中文翻译：

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具有集成光学相干和多光子显微镜的多模态体内皮肤成像

在本文中，我们展示了使用光学相干 (OCM) 和多光子显微镜 (MPM) 对人体皮肤进行高分辨率、多模态体内成像。这两种模式被集成到一个单一的仪器中，以实现同时采集和配准。系统设计和 OCM 图像处理架构使两种模式都具有足够的性能，用于人体皮肤的活体成像。介绍了多模态体内成像的示例以及单个毛细血管循环中血流的延时成像。通过利用多种内在对比度机制，这种集成技术提高了无创可视化活组织的能力。集成的 OCM 和 MPM 在体内诊断各种病理性皮肤病，如皮肤癌、