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Effect of circulating tissue factor on hypercoagulability in type 2 diabetes mellitus studied by rheometry and dielectric blood coagulometry.
Biorheology ( IF 1.1 ) Pub Date : 2016-11-20 , DOI: 10.3233/bir-16107
Isao Uchimura 1 , Makoto Kaibara 2 , Masayuki Nagasawa 3, 4 , Yoshihito Hayashi 5
Affiliation  

BACKGROUND Hypercoagulability in type 2 diabetes mellitus (T2DM) patients increases their risk of cardiovascular diseases. OBJECTIVE The aim of this work was to investigate the hypercoagulation mechanism in T2DM patients in terms of circulating tissue factor (TF). METHODS Whole blood coagulation tests by damped oscillation rheometry and dielectric blood coagulometry (DBCM) were performed. RESULTS The average coagulation time was significantly shorter for T2DM patients than for healthy controls. In vitro addition of either anti-TF or anti-activated factor VII (FVIIa) antibody to hypercoagulable blood samples prolonged coagulation times for one group of patients, while coagulation times remained short for another group. The levels of circulating TF were estimated in the former group by measuring the coagulation times for blood samples from healthy subjects with addition of various concentrations of TF and comparing them with the coagulation times for the group. The results indicated that the levels of circulating TF were on the order of subpicomolar at most. CONCLUSIONS Circulating TF is at least partially responsible for a hypercoagulable group of T2DM patients, while an abnormality in the intrinsic coagulation pathway probably occurs in the other group.

中文翻译:

流变测定法和介电血凝测定法研究循环组织因子对2型糖尿病高凝性的影响。

背景技术2型糖尿病(T2DM)患者中的高凝性增加了他们患心血管疾病的风险。目的这项工作的目的是从循环组织因子(TF)的角度研究T2DM患者的高凝机制。方法采用阻尼振荡流变法和介电血凝法(DBCM)进行全血凝结试验。结果T2DM患者的平均凝血时间明显短于健康对照者。向高凝性血液样本中添加抗TF或抗活化的VII因子(FVIIa)抗体可延长一组患者的凝血时间,而另一组患者的凝血时间仍很短。在前一组中,通过测量健康受试者血液样本的凝血时间并添加各种浓度的TF,并将其与该组的凝血时间进行比较,来估算循环TF的水平。结果表明,循环TF的水平至多为亚皮摩尔级。结论循环TF至少部分负责高凝人群的T2DM患者,而内在凝血途径异常可能发生在另一组人群中。
更新日期:2019-11-01
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