Francisella tularensis is a highly virulent intracellular pathogen with the capacity to infect a variety of hosts including humans. One of the most important proteins involved in F. tularensis virulence and pathogenesis is the protein DsbA. This protein is annotated as a lipoprotein with disulfide oxidoreductase/isomerase activity. Therefore, its interactions with different substrates, including probable virulence factors, to assist in their proper folding are anticipated. We aimed to use the immunopurification approach to find DsbA (gene locus FTS_1067) interacting partners in F. tularensis subsp. holarctica strain FSC200 and compare the identified substrates with proteins which were found in our previous comparative proteome analysis. As a result of our work two FTS_1067 substrates, D-alanyl-D-alanine carboxypeptidase family protein and HlyD family secretion protein, were identified. Bacterial two-hybrid systems were further used to test their relevance in confirming FTS_1067 protein interactions.

中文翻译：

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鉴定FTS_1067蛋白的两种底物-一种非凡的弗朗西斯菌毒力因子。

图拉弗朗西斯菌（Francisella tularensis）是一种高毒力的细胞内病原体，能够感染包括人类在内的多种宿主。D. tulara毒力和发病机理中最重要的蛋白质之一是DsbA。该蛋白被标注为具有二硫键氧化还原酶/异构酶活性的脂蛋白。因此，预期其与不同底物的相互作用，包括可能的毒力因子，以帮助其适当折叠。我们旨在使用免疫纯化方法在杜氏烟草亚种中发现DsbA（基因座FTS_1067）相互作用的伴侣。菌株FSC200，并将鉴定出的底物与我们先前的比较蛋白质组分析中发现的蛋白质进行比较。由于我们的工作，两张FTS_1067基材 鉴定了D-丙氨酰-D-丙氨酸羧肽酶家族蛋白和HlyD家族分泌蛋白。细菌双杂交系统进一步用于测试它们在确认FTS_1067蛋白相互作用中的相关性。