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Neural and non-neural contributions to sexual dimorphism of mid-day sleep inDrosophila melanogaster: a pilot study
Physiological Entomology ( IF 1.5 ) Pub Date : 2016-02-19 , DOI: 10.1111/phen.12134
Mobina Khericha 1 , Jaison B Kolenchery 1 , Eran Tauber 1
Affiliation  

Many of the characteristics associated with mammalian sleep are also observed in Drosophila melanogaster Meigen, making the fruit fly a powerful model organism for studying the genetics of this important process. Included among the similarities is the presence of sexual dimorphic sleep patterns, which, in flies, are manifested as increased mid‐day sleep (‘siesta’) in males compared with females. In the present study, targeted mis‐expression of the genes transformer (tra) and tra2 is used to either feminize or masculinize specific neural and non‐neural tissues in the fly. Feminization of male D. melanogaster using three different GAL4 drivers that are expressed in the mushroom bodies induces a female‐like reduced siesta, whereas the masculinization of females using these drivers triggers the male‐like increased siesta. A similar reversal of sex‐specific sleep is also observed by mis‐expressing tra in the fat body, which is a key tissue in energy metabolism and hormone secretion. In addition, the daily expression levels of takeout, an important circadian clock output gene, are sexually dimorphic. Taken together, these experiments suggest that sleep sexual dimorphism in D. melanogaster is driven by multiple neural and non‐neural circuits, within and outside the brain.

中文翻译:

神经和非神经对黑腹果蝇中午睡眠性别二态性的贡献:一项初步研究

在黑腹果蝇 Meigen 中也观察到许多与哺乳动物睡眠相关的特征,这使得果蝇成为研究这一重要过程的遗传学的强大模式生物。相似之处包括存在两性睡眠模式,在果蝇中,与雌性相比,雄性的中午睡眠(“午睡”)增加。在本研究中,基因转换器 (tra) 和 tra2 的靶向错误表达被用于使果蝇中的特定神经和非神经组织女性化或男性化。使用在蘑菇体中表达的三种不同的 GAL4 驱动器使雄性黑腹果蝇雌性化会导致类似雌性的午睡减少,而使用这些驱动器对雌性的雄性化会触发类似于雄性的午睡增加。通过在脂肪体中错误表达 tra 也观察到类似的性别特异性睡眠逆转,脂肪体是能量代谢和激素分泌的关键组织。此外,重要的生物钟输出基因外卖的日常表达水平是两性的。总之,这些实验表明黑腹果蝇的睡眠性别二态性是由大脑内外的多个神经和非神经回路驱动的。
更新日期:2016-02-19
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