Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L-1 BA, 0.1 mg L-1 NAA, and 20-30 g L-1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L-1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197-199 and 184-187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.

中文翻译：

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利用RAPD和ISSR标记对山茱Corn的适应苗的体外繁殖和遗传稳定性进行评估。

山茱us（白山茱is）是一种重要的观赏灌木，具有广泛的应用，例如重新造林计划和土壤保持系统。山茱wood通过cutting插的无性繁殖可能是缓慢，困难和依赖品种的。因此，开发了一种改进的微繁殖方法。在富含六种不同常量营养素来源的培养基上培养了白色念珠菌'Aurea'和'Elegantissima'的节茎段。培养基中补充了N6-苄腺嘌呤（BA）或噻二唑隆（TDZ）与1-萘乙酸（NAA）的组合。无论哪个品种，在pH 6.2的Lloyd和McCown培养基（木本植物培养基（WPM））上均观察到最佳的芽增殖，该培养基含有1.0 mg L-1 BA，0.1 mg L-1 NAA和20-30 g L- 1个蔗糖。当测试不同浓度的NAA或吲哚-3-丁酸（IBA）时，可以通过体外方法实现再生芽的生根。将微切屑在富含0.25 mg L-1 NAA的培养基上生根8周，并放入温室的P9容器中。20周后，植物的最终存活率对于“ Aurea”为80％，对于“ Elegantissima”为90％。通过使用两种基于DNA的分子标记技术，证实了微繁殖植株的遗传稳定性。总共30个随机扩增多态性DNA（RAPD）和20个简单序列间重复（ISSR）引物分别在'Aurea'和'Elegantissima'植株中产生了197-199和184-187个不同的和可重复的条带类型。所有的RAPD和ISSR谱都是单态的，并且与母本相当。