A method for selectively inducing apoptosis in tumor nodules is presented, with close-to-cellular level resolution, using 3D-resolved widefield temporal focusing illumination. Treatment times on the order of seconds were achieved using Verteporfin as the photosensitizer, with doses of 30 μg ml-1 and below. Results were achieved on both 2D and 3D cell cultures, demonstrating that treatment was possible through approximately one hundred microns of dense tumor nodules.

中文翻译：

---

使用时间聚焦的光动力疗法的 3D 分辨靶向

提出了一种选择性诱导肿瘤结节细胞凋亡的方法，具有接近细胞水平的分辨率，使用 3D 分辨率的宽场时间聚焦照明。使用 Verteporfin 作为光敏剂，剂量为 30 μg ml-1 及以下，达到了数秒级的治疗时间。结果在 2D 和 3D 细胞培养物上都得到了证实，证明通过大约 100 微米的致密肿瘤结节进行治疗是可能的。