We have developed a programmable illumination system capable of tracking and illuminating numerous objects simultaneously using only low-cost and reused optical components. The active feedback control software allows for a closed-loop system that tracks and perturbs objects of interest automatically. Our system uses a static stage where the objects of interest are tracked computationally as they move across the field of view allowing for a large number of simultaneous experiments. An algorithmically determined illumination pattern can be applied anywhere in the field of view with simultaneous imaging and perturbation using different colors of light to enable spatially and temporally structured illumination. Our system consists of a consumer projector, camera, 35-mm camera lens, and a small number of other optical and scaffolding components. The entire apparatus can be assembled for under $4,000.

中文翻译：

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具有主动反馈的廉价可编程照明显微镜

我们开发了一种可编程照明系统，能够仅使用低成本和重复使用的光学组件同时跟踪和照明多个物体。主动反馈控制软件允许闭环系统自动跟踪和干扰感兴趣的对象。我们的系统使用静态阶段，当感兴趣的物体在视野中​​移动时，通过计算跟踪它们，从而允许进行大量的同时实验。算法确定的照明模式可以应用于视野中的任何地方，同时使用不同颜色的光进行成像和扰动，以实现空间和时间结构化照明。我们的系统由消费类投影仪、相机、35 毫米相机镜头和少量其他光学和脚手架组件组成。