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Mitosomal chaperone modulation during the life cycle of the pathogenic protist Giardia intestinalis.
European Journal of Cell Biology ( IF 6.6 ) Pub Date : 2016-09-10 , DOI: 10.1016/j.ejcb.2016.08.005
Victor Midlej 1 , Luciana Penha 2 , Rosane Silva 2 , Wanderley de Souza 3 , Marlene Benchimol 4
Affiliation  

The mitosome is a double-membrane enveloped organelle that is found in few unicellular eukaryotes, one of which is the human intestinal parasitic protist Giardia intestinalis, which also lacks mitochondria and peroxisomes. This flagellated protist grows in vitro as trophozoites and under some conditions, differentiates into cysts, which are characterized by the absence of externalized flagella, a round shape, and the presence of a cyst wall. The presence and distribution of mitosomal proteins, such as giardial iron-sulfur cluster protein (GiIscU), heat-shock protein 70 (mit-HSP70) and giardial chaperonin 60 (GiCpn60), during the process of trophozoite-to-cyst transformation was tracked using confocal laser scanning microscopy and western blotting. During the early stages of the differentiation process (∼12h), there was a significant decrease in the extent of chaperone labeling in the cells, which disappeared after 21h but was recovered during the cyst stage; IscU labeling remained present throughout the differentiation process. This finding was confirmed by mRNA expression analysis, thus indicating that a process modulates the expression of mitosomal chaperones during the G. intestinalis life cycle. Microscopy techniques, such as structured illumination and electron tomography, revealed a novel profile for central mitosomes, as well as the presence of both rounded and elongated mitosomes.

中文翻译:

致病性原生动物贾第鞭毛虫的生命周期中的线粒体伴侣调节。

线粒体是在很少的单细胞真核生物中发现的双膜包被的细胞器,其中之一是人肠寄生的原生生物贾第鞭毛虫,它也缺乏线粒体和过氧化物酶体。这种带鞭毛的原生生物在体外以滋养体的形式生长,并在某些条件下分化为囊肿,其特征是没有外部鞭毛,圆形和囊壁。跟踪了滋养体向囊肿转化过程中线粒体蛋白(如贾第虫铁硫簇蛋白(GiIscU),热休克蛋白70(mit-HSP70)和贾第伴侣蛋白60(GiCpn60)的存在和分布。使用共聚焦激光扫描显微镜和蛋白质印迹。在分化过程的早期阶段(约12小时),细胞中伴侣标记的程度显着降低,在21h后消失,但在囊肿期恢复。IscU标记在整个分化过程中仍然存在。mRNA表达分析证实了这一发现,从而表明在肠小肠结肠菌生命周期中,有一个过程调节了线粒体伴侣的表达。显微技术,例如结构化照明和电子断层扫描,揭示了中心线粒体的新特征,以及圆形和细长线粒体的存在。因此,表明该过程调节了肠球菌生命周期中线粒体伴侣的表达。显微技术,例如结构化照明和电子断层扫描,揭示了中心线粒体的新特征,以及圆形和细长线粒体的存在。因此,表明该过程调节了肠球菌生命周期中线粒体伴侣的表达。显微技术,例如结构化照明和电子断层扫描,揭示了中心线粒体的新特征,以及圆形和细长线粒体的存在。
更新日期:2019-11-01
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