Eye-derived fluids, including tears, aqueous humour and vitreous humour often contain molecular signatures of ocular disease states. These signatures can be composed of cytokines, chemokines, growth factors, proteases and soluble receptors. However, the small quantities (<10 µl) of these fluids severely limit the detection of these proteins by traditional enzyme-linked immunosorbent assay or Western blot. To maximise the amount of information generated from the analysis of these specimens, many researchers have employed multiplex immunoassay technologies for profiling the expression or modification of multiple proteins from minute sample volumes.

中文翻译：

---

多重免疫分析技术在眼部疾病调查中的应用

眼源性液体，包括眼泪、房水和玻璃体液，通常含有眼部疾病状态的分子特征。这些特征可以由细胞因子、趋化因子、生长因子、蛋白酶和可溶性受体组成。然而，这些液体的少量（<10 µl）严重限制了传统酶联免疫吸附测定或蛋白质印迹法对这些蛋白质的检测。为了最大限度地从这些标本的分析中产生的信息量，许多研究人员已经采用多重免疫测定技术来分析来自微小样本量的多种蛋白质的表达或修饰。