We have established a long-term, stable primary chick forebrain neuron (FBN) culture on a microelectrode array platform as a biosensor system for neurotoxicant screening and for neuroelectrophysiological studies for multiple purposes. This paper reports some of our results, which characterize the biosensor pharmacologically. Dose-response experiments were conducted using NMDA receptor antagonist AP5 and GABAA receptor agonist musimol (MUS). The chick FBN biosensor (C-FBN-biosensor) responds to the two agents in a pattern similar to that of rodent counterparts; the estimated EC50s (the effective concentration that causes 50% inhibition of the maximal effect) are 2.3 μM and 0.25 μM, respectively. Intercultural and intracultural reproducibility and long-term reusability of the C-FBN-biosensor are addressed and discussed. A phenomenon of sensitization of the biosensor that accompanies intracultural reproducibility in paired dose-response experiments for the same agent (AP5 or MUS) is reported. The potential application of the C-FBN-biosensor as an alternative to rodent biosensors in shared sensing domains (NMDA receptor and GABAA receptor) is suggested.

中文翻译：

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基于微电极阵列的小鸡前脑神经元生物传感器如何响应谷氨酸NMDA受体拮抗剂AP5和GABAA受体拮抗剂Musimol。

我们已经在微电极阵列平台上建立了长期，稳定的原代小鸡前脑神经元（FBN）培养物，作为用于神经毒性筛选和用于多种目的的神经电生理研究的生物传感器系统。本文报告了我们的一些结果，这些结果在生物学上描述了生物传感器。使用NMDA受体拮抗剂AP5和GABAA受体激动剂musimol（MUS）进行剂量反应实验。雏鸡FBN生物传感器（C-FBN-biosensor）对两种药物的反应方式与啮齿动物对应物相似。估计的EC50（引起最大作用抑制50％的有效浓度）分别为2.3μM和0.25μM。C-FBN生物传感器的跨文化和文化内的可重复性和长期可重用性得到了讨论。据报道，在同一试剂（AP5或MUS）的成对剂量反应实验中，伴随着文化内再现性的生物传感器的增敏现象。建议在共享的感测域（NMDA受体和GABAA受体）中，将C-FBN生物传感器作为啮齿动物生物传感器的替代品的潜在应用。