To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C₆-NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by ²H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C₆-NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C₆-NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules.

为了研究荧光探针对膜性能的影响，我们研究了由1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC）和1-棕榈酰基2-油酰基-sn-甘油-3-磷酸胆碱组成的模型膜。（POPC）在存在和不存在荧光探针的情况下。使用NBD-DOPE或C ₆ -NBD-PC作为探针，通过荧光显微镜观察到巨型单层囊泡（GUV）的形态随温度和成分的变化而变化。通过² H-NMR光谱研究不含荧光探针的模型膜的相行为。我们发现，在GUV上观察到的亮相是富含POPC的液相，而暗相是富含DPPC的凝胶相。NBD-DOPE和C ₆当GUV处于凝胶+液相共存状态时，-NBD-PC优先参与液相域。包含两个荧光探针（1摩尔％）降低了POPC / DPPC膜的转变温度。另外，C ₆ -NBD-PC显示出比NBD-DOPE更强的作用，这被认为与荧光分子的结构有关。