A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza-s-indacene-8-yl (Me4-BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus; the enzymatic yield has been raised to 50–70% by appliance of organic solvent in the incubation medium.

中文翻译：

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荧光标记的神经酰胺-1-磷酸类似物的便利合成

在脂肪酸残基的共位处带有 9-蒽乙烯基或 4,4-二氟-3a,4a-二氮杂-s-indacene-8-yl (Me4-BODIPY) 荧光团的神经酰胺-1-磷酸酯的荧光类似物的合成是执行。合成的关键阶段是在来自色斑链霉菌(Streptomyces chromofuscus)的磷脂酶D催化下水解相应的鞘磷脂；通过在培养基中加入有机溶剂，酶促产率提高到 50-70%。