Damage to cellular DNA is believed to determine the cytotoxicity of oxaliplatin. However, high resolution structures formed by oxaliplatin and different linear DNA remain unclear. This study characterized, the key structures of different linear DNA in the platination process by UV absorption spectra and atomic force microscopy (AFM). Bathochromic shift and hyperchromicity in UV spectra after addition of oxaliplatin revealed that it can disrupt base stacking of DNA in the platination process. AFM results of different linear DNA indicated that, the platination process can induce DNA change from an extended conformation to the network structure with many kinks and finally to the compact particles, or toroids with increasing the incubation time. All AFM results confirmed that, platination of different linear DNA by oxaliplatin is a time depended process. The present AFM results provide, structural evidence about the interactions between oxaliplatin and different linear DNA containing multiple targets. SCANNING 38:880-888, 2016. © 2016 Wiley Periodicals, Inc.

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奥沙利铂与线性DNA相互作用的结构研究

据信对细胞 DNA 的损伤决定了奥沙利铂的细胞毒性。然而，奥沙利铂和不同线性 DNA 形成的高分辨率结构仍不清楚。本研究通过紫外吸收光谱和原子力显微镜 (AFM) 表征了铂化过程中不同线性 DNA 的关键结构。添加奥沙利铂后紫外光谱的红移和超色度表明它可以破坏铂化过程中 DNA 的碱基堆积。不同线性DNA的AFM结果表明，随着孵育时间的增加，铂化过程可以诱导DNA从延伸构象转变为具有许多扭结的网络结构，最终转变为致密的颗粒或环形。所有 AFM 结果都证实，奥沙利铂对不同线性 DNA 的铂化是一个时间依赖的过程。目前的 AFM 结果提供了关于奥沙利铂和含有多个目标的不同线性 DNA 之间相互作用的结构证据。扫描 38:880-888, 2016. © 2016 Wiley Periodicals, Inc.