ABSTRACT Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3,5-dihydroxy-2-(1-p-mentheneyl)-trans-stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns. GRAPHICAL ABSTRACT

中文翻译：

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一种单一标记物同时定量分析灵芝主要成分

摘要 采用单标记物(QAMS) 方法建立了多组分定量分析方法进行质量评价，并通过同时对白木兰中四种主要成分进行定量分析来验证其可行性。选择松菌素、松香素、松香素和 3,5-二羟基-2-(1-p-薄荷烯基)-反式-二苯乙烯的四种主要成分作为分析物，通过 RP-HPLC 和 UV 检测器来评估质量。通过比较外标法和 QAMS 与不同 HPLC 系统的定量结果来评估该方法。结果表明，经外标法和QAMS测定的灵芝四种含量的定量结果均无显着差异（RSD<3%）。四种分析物的含量（松香素、松香素、灵芝中的pinostrobin和Reflexanbene I)是通过pinosylvin的单一标记物测定的。该指纹是由配备三种不同色谱柱的 Shimadzu LC-20AT 和 Waters e2695 HPLC 测定的光谱。图形概要