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Laboratory identification of anaerobic bacteria isolated on Clostridium difficile selective medium.
Acta Microbiologica et Immunologica Hungarica ( IF 1.5 ) Pub Date : 2016-6-30 , DOI: 10.1556/030.63.2016.2.3
Cristina Rodriguez 1 , Nathalie Warszawski 1 , Nicolas Korsak 1 , Bernard Taminiau 1 , Johan Van Broeck 2 , Michel Delmée 2 , Georges Daube 1
Affiliation  

Despite increasing interest in the bacterium, the methodology for Clostridium difficile recovery has not yet been standardized. Cycloserine-cefoxitin fructose taurocholate (CCFT) has historically been the most used medium for C. difficile isolation from human, animal, environmental, and food samples, and presumptive identification is usually based on colony morphologies. However, CCFT is not totally selective. This study describes the recovery of 24 bacteria species belonging to 10 different genera other than C. difficile, present in the environment and foods of a retirement establishment that were not inhibited in the C. difficile selective medium. These findings provide insight for further environmental and food studies as well as for the isolation of C. difficile on supplemented CCFT.

中文翻译:

在艰难梭菌选择性培养基上分离的厌氧细菌的实验室鉴定。

尽管人们对该细菌越来越感兴趣,但艰难梭菌回收的方法尚未标准化。昔洛昔芬-头孢西丁果糖牛磺胆酸盐(CCFT)一直以来是从人,动物,环境和食物样品中分离艰难梭菌的最常用培养基,推定性鉴定通常基于菌落形态。但是,CCFT并非完全具有选择性。这项研究描述了艰难梭菌选择性培养基中未抑制的,存在于环境和退休场所食品中的艰难梭菌以外的10个不同属的24种细菌的回收。这些发现为进一步的环境和食品研究以及补充CCFT分离艰难梭菌提供了见识。
更新日期:2020-08-21
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