Adult stem cells (ASCs) are undifferentiated cells found throughout the body that divide to replenish dying cells and regenerate damaged tissues, which are the powerful sources for cell therapy and tissue engineering. Bone marrow-derived mesenchymal stem cells (BMSCs), adipose tissue-derived mesenchymal stem cells (ADSCs), and peripheral blood monocytes (PBMCs) are the common ASCs, and many studies indicated that ASCs isolated from various adult tissues could be induced to hepatocyte-like cells in vitro. However, the isolation, culture protocols, characterization of ASCs and hepatocyte-like cells are different. This review aims to describe the isolation and culture procedures for ASCs, to summarize the molecular characterization of ASCs, to characterize function of hepatocyte-like cells, and to discuss the future role of ASCs in cell therapy and tissue engineering.

中文翻译：

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简洁评论：体外将成人成体干细胞分化为类肝细胞。

成体干细胞（ASC）是遍布全身的未分化细胞，它们分裂后可补充垂死的细胞并再生受损的组织，这是细胞疗法和组织工程的强大动力。骨髓间充质干细胞（BMSC），脂肪组织间充质干细胞（ADSC）和外周血单核细胞（PBMC）是常见的ASC，许多研究表明，从各种成人组织中分离出的ASC均可被诱导为肝细胞样细胞。但是，ASC和肝细胞样细胞的分离，培养方案，特征不同。这篇综述旨在描述ASC的分离和培养程序，总结ASC的分子特征，以表征肝细胞样细胞的功能，