Nematodes such as Caenorhabditis elegans offer a very effective and tractable system to probe the underlying mechanisms of diverse sensory behaviors. Numerous platforms exist for quantifying nematode behavior and often require separate dependencies or software. Here I describe a novel and simple tool called NemaCount that provides a versatile solution for the quantification of nematode chemotaxis behavior. The ease of installation and user-friendly interface makes NemaCount a practical tool for measuring diverse behaviors and image features of nematodes such as C. elegans. The main advantage of NemaCount is that it operates from within a modern browser such as Google Chrome or Apple Safari. Any features that change in total number, size, shape, or angular distance between control and experimental preparations are suited to NemaCount for image analysis, while commonly used chemotaxis assays can be quantified, and statistically analyzed using a suite of functions from within NemaCount. NemaCount also offers image filtering options that allow the user to improve object detection and measurements. NemaCount was validated by examining nematode chemotaxis behavior; angular distances of locomotory tracks in C. elegans; and body lengths of Heterorhabditis bacteriophora nematodes. Apart from a modern browser, no additional software is required to operate NemaCount, making NemaCount a cheap, simple option for the analysis of nematode images and chemotaxis behavior.

中文翻译：

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NemaCount：定量浏览器中的线虫趋化行为。

线虫（如秀丽隐杆线虫）提供了一个非常有效且易于处理的系统，可以探究各种感官行为的潜在机制。存在许多用于量化线虫行为的平台，并且通常需要单独的依赖项或软件。在这里，我描述了一种称为NemaCount的新颖，简单的工具，该工具为定量线虫趋化行为提供了一种通用的解决方案。易于安装和友好的用户界面使NemaCount成为测量线虫（例如秀丽隐杆线虫）各种行为和图像特征的实用工具。NemaCount的主要优点是可以在现代浏览器（例如Google Chrome或Apple Safari）中运行。对照和实验制剂之间总数，大小，形状或角距离变化的任何特征都适合NemaCount进行图像分析，而常用的趋化性测定可以通过NemaCount内部的一组函数进行量化和统计分析。NemaCount还提供图像过滤选项，使用户可以改善物体检测和测量。通过检查线虫趋化行为验证了NemaCount；秀丽隐线虫运动轨迹的角距离; 细菌性杂多菌的体长和体长线虫。除现代浏览器外，无需其他软件即可操作NemaCount，这使NemaCount成为分析线虫图像和趋化行为的廉价，简单的选择。