Deciphering the many interactions that occur between a virus and host cell over the course of infection is paramount to understanding mechanisms of pathogenesis and to the future development of antiviral therapies. Over the past decade, researchers have started to understand these complicated relationships through the development of methodologies, including advances in RNA interference, proteomics, and the development of genetic tools such as haploid cell lines, allowing high-throughput screening to identify critical contact points between virus and host. These advances have produced a wealth of data regarding host factors hijacked by viruses to promote infection, as well as antiviral factors responsible for subverting viral infection. This review highlights findings from virus-host screens and discusses our thoughts on the direction of screening strategies moving forward.

中文翻译：

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病毒与宿主的相互作用：从无偏见的遗传筛选到功能。

理解在感染过程中病毒与宿主细胞之间发生的许多相互作用对于理解发病机理和抗病毒疗法的未来发展至关重要。在过去的十年中，研究人员已开始通过方法学的发展来理解这些复杂的关系，包括RNA干扰，蛋白质组学的发展以及诸如单倍体细胞系等遗传工具的发展，从而可以通过高通量筛选来识别之间的关键接触点。病毒和宿主。这些进展产生了大量有关病毒劫持以促进感染的宿主因子以及负责破坏病毒感染的抗病毒因子的大量数据。