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Application of ATP-based bioluminescence for bioaerosol quantification: Effect of sampling method
Journal of Aerosol Science ( IF 4.5 ) Pub Date : 2015-12-01 , DOI: 10.1016/j.jaerosci.2015.08.003
Taewon Han 1 , Melody Wren 1 , Kelsey DuBois 1 , Jennifer Therkorn 1 , Gediminas Mainelis 1
Affiliation  

An adenosine triphosphate (ATP)-based bioluminescence has potential to offer a quick and affordable method for quantifying bioaerosol samples. Here we report on our investigation into how different bioaerosol aerosolization parameters and sampling methods affect bioluminescence output per bacterium, and implications of that effect for bioaerosol research. Bacillus atrophaeus and Pseudomonas fluorescens bacteria were aerosolized by using a Collison nebulizer (BGI Inc., Waltham, MA) with a glass or polycarbonate jar and then collected for 15 and 60 min with: (1) Button Aerosol Sampler (SKC Inc., Eighty Four, PA) with polycarbonate, PTFE, and cellulose nitrate filters, (2) BioSampler (SKC Inc.) with 5 and 20 mL of collection liquid, and (3) our newly developed Electrostatic Precipitator with Superhydrophobic Surface (EPSS). For all aerosolization and sampling parameters we compared the ATP bioluminescence output per bacterium relative to that before aerosolization and sampling. In addition, we also determined the ATP reagent storage and preparation conditions that that do not affect the bioluminescence signal intensity. Our results show that aerosolization by a Collison nebulizer with a polycarbonate jar yields higher bioluminescence output per bacterium compared to the glass jar. Interestingly enough, the bioluminescence output by P. fluorescens increased substantially after its aerosolization compared to the fresh liquid suspension. For both test microorganisms, the bioluminescence intensity per bacterium after sampling was significantly lower than that before sampling suggesting negative effect of sampling stress on bioluminescence output. The decrease in bioluminescence intensity was more pronounces for longer sampling times and significantly and substantially depended on the sampling method. Among the investigated method, the EPSS was the least injurious for both microorganisms and sampling times. While the ATP-based bioluminescence offers a quick bioaerosol sample analysis method, this works demonstrates that the method output depends on bioaerosol generation and sampling methods, as well as reagent storage.

中文翻译:

基于 ATP 的生物发光在生物气溶胶定量中的应用:采样方法的影响

基于三磷酸腺苷 (ATP) 的生物发光有可能为生物气溶胶样品的定量提供一种快速且经济实惠的方法。在这里,我们报告了我们对不同生物气溶胶雾化参数和采样方法如何影响每个细菌的生物发光输出的调查,以及这种影响对生物气溶胶研究的影响。使用 Collison 雾化器(BGI Inc., Waltham, MA)和玻璃或聚碳酸酯罐将萎缩芽孢杆菌和荧光假单胞菌雾化,然后用以下方法收集 15 和 60 分钟:(1)按钮气溶胶采样器(SKC Inc.,80四、PA) 带有聚碳酸酯、PTFE 和硝酸纤维素过滤器,(2) 带有 5 和 20 毫升收集液的生物采样器 (SKC Inc.),以及 (3) 我们新开发的具有超疏水表面 (EPSS) 的静电除尘器。对于所有雾化和采样参数,我们比较了每个细菌的 ATP 生物发光输出与雾化和采样之前的比较。此外,我们还确定了不影响生物发光信号强度的 ATP 试剂储存和制备条件。我们的结果表明,与玻璃罐相比,带有聚碳酸酯罐的 Collison 雾化器的雾化产生每个细菌更高的生物发光输出。有趣的是,与新鲜液体悬浮液相比,荧光假单胞菌的生物发光输出在雾化后显着增加。对于两种测试微生物,采样后每个细菌的生物发光强度显着低于采样前,表明采样压力对生物发光输出的负面影响。对于较长的采样时间,生物发光强度的降低更为明显,并且在很大程度上取决于采样方法。在所研究的方法中,EPSS 对微生物和采样时间的危害最小。虽然基于 ATP 的生物发光提供了一种快速的生物气溶胶样品分析方法,但这项工作表明该方法的输出取决于生物气溶胶的生成和采样方法,以及试剂存储。
更新日期:2015-12-01
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