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The diversity of endophytic fungi in the above-ground tissue of two Lycopodium species in Poland.
Symbiosis ( IF 2.5 ) Pub Date : 2014-09-10 , DOI: 10.1007/s13199-014-0291-1
Julia Pawłowska 1 , Mateusz Wilk 2 , Anna Sliwińska-Wyrzychowska 3 , Monika Mętrak 4 , Marta Wrzosek 1
Affiliation  

Endophytes are a large and diverse group of fungi that colonize healthy plant tissues without causing any symptoms. The majority of studies have focused on angiosperm and conifer hosts and few have examined the endophytes of lycophytes. In the present study, we characterized culturable endophytic fungi in two closely related Lycopodium species (L. annotinum and L. clavatum) from pine, beech, oak and spruce forests across Poland. More than 400 strains were isolated but only 18 Ascomycete species were identified. Members of the Dothideomycetes dominated the fungal endophyte communities in Lycopodium. The most abundant taxa cultured were Phoma brasiliensis (from L. clavatum) and Paraconiothyrium lycopodinum (from L. annotinum). Five taxa were isolated exclusively from L. annotinum, but only two of them (Paraconiothyrium lycopodinum and Mycosphaerella sp.) were relatively abundant. Two taxa were only found in L. clavatum, namely: Stagonospora pseudovitensis and an unidentified Dothideomycete. The taxon assigned as Ascomycota 2 (SH219457.06FU) was isolated only from strobili of both host species. Direct PCR and cloning from L. annotinum shoots revealed a substantially greater endophyte richness compared with the results from culturing.

中文翻译:

波兰两种石松属植物地上组织内生真菌的多样性。

内生菌是一大群种类繁多的真菌,它们在健康的植物组织中定殖而不会引起任何症状。大多数研究都集中在被子植物和针叶树宿主上,很少研究石松植物的内生菌。在本研究中,我们对来自波兰松树、山毛榉、橡树和云杉林的两种密切相关的石松属物种(L. annotinumL. clavatum)中的可培养内生真菌进行了表征。分离出 400 多种菌株,但仅鉴定了 18 种子囊菌。Dothideomycetes 的成员在石松属的真菌内生菌群落中占主导地位。培养的最丰富的分类群是Phoma brasiliensis(来自L. clavatum)和Paraconiothyrium lycopodinum(来自L. annotinum)。五个分类群完全从L. annotinum中分离出来,但其中只有两个(Paraconiothyrium lycopodinumMycosphaerella sp.)相对丰富。两种分类群仅在L. clavatum 中发现,即:Stagonospora pseudovitensis和一种身份不明的 Dothideomycete。指定为子囊菌门 2 (SH219457.06FU) 的分类群仅从两种宿主物种的 strobili 中分离出来。从L. annotinum枝条进行的直接 PCR 和克隆显示,与培养结果相比,内生菌丰富度明显更高。
更新日期:2014-09-10
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