This article elucidates that strain Pseudomonas aeruginosa (IES-Ps-1) is a versatile toxic organic compound degrader. With the degradation of malathion and cypermethrin (studied by other researchers previously), this strain was able to degrade phenol. Two other indigenous soil flora (i.e., Pseudomonas sp. (IES-S) and Bacillus subtilis (IES-B)) were also found to be potential phenol degraders. Phenol was degraded with Monod kinetics during growth in nutrient broth and mineral salts medium. Before entering into the growth inhibition phase, strains IES-Ps-1, IES-S and IES-B could tolerate up to 400, 700 and 500 mg/L phenol, respectively, when contained in nutrient broth. However, according to the Luong–Levenspiel model, the growth of strains IES-Ps-1, IES-S and IES-B would cease at 2000, 2174 and 2190 mg/L phenol, respectively. Strain IES-Ps-1 degraded 700, 900 and 1050 mg/L phenol contained in mineral salts medium with the specific rates of 0.034, 0.075 and 0.021 h−1, respectively. All these strains grew by making clusters when exposed to phenol in order to prevent damages due to high substrate concentration. These strains transformed phenol into catechol, which was then degraded via ortho-cleavage pathway.

中文翻译：

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假单胞菌和芽孢杆菌对苯酚的降解动力学及途径

本文阐明了铜绿假单胞菌 (IES-Ps-1) 菌株是一种多功能的有毒有机化合物降解剂。随着马拉硫磷和氯氰菊酯的降解（其他研究人员之前研究过），该菌株能够降解苯酚。另外两种本土土壤菌群（即假单胞菌属（IES-S）和枯草芽孢杆菌（IES-B））也被发现是潜在的苯酚降解剂。在营养肉汤和矿物盐培养基中生长期间，苯酚被莫诺动力学降解。在进入生长抑制阶段之前，当包含在营养肉汤中时，菌株 IES-Ps-1、IES-S 和 IES-B 可分别耐受高达 400、700 和 500 mg/L 的苯酚。然而，根据 Luong-Levenspiel 模型，菌株 IES-Ps-1、IES-S 和 IES-B 的生长将分别在 2000、2174 和 2190 mg/L 苯酚时停止。菌株 IES-Ps-1 分别以 0.034、0.075 和 0.021 h-1 的比速率降解矿物盐培养基中含有的 700、900 和 1050 mg/L 苯酚。所有这些菌株在暴露于苯酚时都通过形成簇来生长，以防止由于高底物浓度造成的损害。这些菌株将苯酚转化为儿茶酚，然后通过正切途径降解。