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Evaluation of Structure and Assembly of Xyloglucan from Tamarind Seed (Tamarindus indica L.) with Atomic Force Microscopy.
Food Biophysics ( IF 3 ) Pub Date : 2015-04-12 , DOI: 10.1007/s11483-015-9395-2
Arkadiusz Kozioł 1 , Justyna Cybulska 1 , Piotr M Pieczywek 1 , Artur Zdunek 1
Affiliation  

The role of xyloglucan (XG) in the cell wall of plants and its technological usability depends on several factors, pertaining to molecular structure. Therefore, the goal of this study was to evaluate the nano-structure and self-assembly of XG by atomic force microscopy (AFM). As the model, a non-modified xyloglucan from a tamarind seed (Tamarindus indica L.) was used. Samples were minimally processed, i.e., treated with low-power ultrasound and studied on the surface of mica in ambient butanol. AFM topographic images revealed rod-like nanomolecules of xyloglucan with a mean height of 2.3 ± 0.5 nm and mean length of 640 ± 360 nm. The AFM study also showed that XG chains possessed a helical structure with a period of 115.8 ± 29.2 nm. This study showed possible-bending of molecules with a mean angle of 127.8 ± 25.6°. The xyloglucan molecules were able to aggregate as cross-like and a parallel like assemblies, and possibly as rope-like structures. The self-assembled bundles of xyloglucan chains were often complexed at an angle of 114.2 ± 36.3°.

中文翻译:

用原子力显微镜评价罗望子种子(Tamarindus indica L.)中木葡聚糖的结构和组装。

木葡聚糖(XG)在植物细胞壁中的作用及其技术可用性取决于与分子结构有关的几个因素。因此,本研究的目的是通过原子力显微镜(AFM)评估XG的纳米结构和自组装。作为模型,来自罗望子种子的非修饰木葡聚糖(Tamarindus indicaL.)被使用。对样品进行最少的处理,即用低功率超声处理,然后在环境丁醇中的云母表面上进行研究。AFM地形图显示木葡聚糖棒状纳米分子,平均高度为2.3±0.5 nm,平均长度为640±360 nm。AFM研究还表明,XG链具有螺旋结构,周期为115.8±29.2 nm。这项研究表明平均角度为127.8±25.6°的分子可能会弯曲。木葡聚糖分子能够聚集为十字状和平行状组装体,并且可能聚集为绳状结构。自组装的木葡聚糖链束通常以114.2±36.3°的角度复合。
更新日期:2015-04-12
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