Inositol is a 6-carbon sugar alcohol that has been shown in limited studies to reduce retinopathy of prematurity and chronic lung disease in premature newborns. Developmentally it has a high concentration in the fetus that decreases with gestational age. It is transported from the fetus to the mother across the placenta. Although studies are underway to determine inositol kinetics in premature newborns treated therapeutically, the effects of gestational age, age after birth, and feeding on inositol concentrations after birth have not been studied adequately in premature newborns. Such studies would minimize blood removal and trauma in preterm newborns by using plasma samples scavenged from the clinical laboratory to measure inositol after birth, if they remain stable. This report describes a new high pressure liquid chromatographic assay for inositol and its use to study the stability of inositol in conditions of storage that might be encountered within the clinical laboratory. The assay is linear from 0 to 1000 Mm with a lower limit of quantitation of 50 μM. Inositol in human plasma remains stable in refrigeration and at room temperature for up to 14 days and is not affected by storage in red blood cells that are intact or lysed. Anticoagulants encountered in clinical blood samples do not interfere with the chromatograms. Thus, it is feasible to measure the changes in inositol concentrations in plasma from preterm newborns that is scavenged from the clinical laboratory after storage for as long as 14 days.

中文翻译：

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HPLC分析肌醇及其在清除样品条件下的稳定性。

肌醇是一种6碳糖醇，在有限的研究中已经显示出它可以减少早产儿的视网膜病变和早产儿的慢性肺部疾病。从发育上讲，它在胎儿中的浓度很高，随着胎龄的增加而降低。它通过胎盘从胎儿运输到母亲。尽管目前正在进行研究以确定经治疗的早产儿肌醇的动力学，但尚未对早产儿的胎龄，出生后年龄以及出生后喂养对肌醇浓度的影响进行充分研究。此类研究将通过使用从临床实验室清除的血浆样本（如果出生后仍保持稳定）来测量肌醇，从而使早产新生儿的血液去除和创伤最小化。本报告介绍了一种新的肌醇高压液相色谱测定方法，并用于研究临床实验室可能遇到的贮存条件下肌醇的稳定性。该测定在0至1000 Mm之间呈线性，定量下限为50μM。人血浆中的肌醇在冷藏和室温下最多可保持稳定14天，并且不受保存在完整或裂解的红细胞中的影响。临床血液样本中遇到的抗凝剂不会干扰色谱图。因此，测量储存长达14天后从临床实验室清除的早产儿血浆中肌醇浓度的变化是可行的。