The hyperthermophilic, sulfate-reducing archaeon, Archaeoglobus fulgidus, utilizes CO as an energy source and it is resistant to the toxic effects of high CO concentrations. Herein, transcription profiles were obtained from A. fulgidus during growth with CO and sulfate or thiosulfate, or without an electron acceptor. This provided a basis for a model of the CO metabolism of A. fulgidus. The model suggests proton translocation by “Mitchell-type” loops facilitated by Fqo catalyzing a :menaquinone oxidoreductase reaction, as the major mode of energy conservation, rather than formate or H₂ cycling during respiratory growth. The bifunctional CODH (cdhAB-2) is predicted to play an ubiquitous role in the metabolism of CO, and a novel nitrate reductase-associated respiratory complex was induced specifically in the presence of sulfate. A potential role of this complex in relation to and APS reduction is discussed. Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. Finally, the FqoF subunit may catalyze a :F₄₂₀ oxidoreductase reaction. In the absence of electron acceptor, downregulation of F₄₂₀H₂ dependent steps of the acetyl-CoA pathway is linked to transient formate generation. Overall, carboxidotrophic growth seems as an intrinsic capacity of A. fulgidus with little need for novel resistance or respiratory complexes.

中文翻译：

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通过比较转录组分析评估还原超嗜热硫酸盐的古细菌古细菌fulgidus VC-16的一氧化碳代谢。

高温还原硫酸盐古生菌，古菌，利用一氧化碳作为能源，对高浓度一氧化碳的毒性作用具有抵抗力。本文中，在有CO和硫酸盐或硫代硫酸盐，或没有电子受体的生长过程中，从烟曲霉获得了转录谱。这为A.fulgidus的CO代谢模型提供了基础。该模型表明，通过Fqo催化：甲萘醌氧化还原酶反应，作为能量保存的主要方式，质子通过“ Mitchell型”环易位，而不是在呼吸生长过程中甲酸盐或H ₂循环。双功能CODH（cdhAB-2）预计在CO的代谢中起普遍作用，并且在硫酸盐的存在下特异性诱导了新的硝酸盐还原酶相关的呼吸复合物。讨论了与降低APS有关的这种复合物的潜在作用。多个膜结合的异二硫键还原酶（DsrMK）可以促进节能和非节能的薄荷脑氧化。最后，FqoF亚基可以催化：F ₄₂₀氧化还原酶反应。在没有电子受体的情况下，乙酰辅酶AA途径中F ₄₂₀ H ₂依赖性步骤的下调与瞬时甲酸的产生有关。总体而言，羧化营养的生长似乎是A. fulgidus的内在能力。 几乎不需要新型抗药性或呼吸系统复合物。