Effect of DNA extraction procedure, repeated extraction and ethidium monoazide (EMA)/propidium monoazide (PMA) treatment on overall DNA yield and impact on microbial fingerprints for bacteria, fungi and archaea in a reference soil,Applied Soil Ecology

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Effect of DNA extraction procedure, repeated extraction and ethidium monoazide (EMA)/propidium monoazide (PMA) treatment on overall DNA yield and impact on microbial fingerprints for bacteria, fungi and archaea in a reference soil
Applied Soil Ecology ( IF 4.8 ) Pub Date : 2015-09-01 , DOI: 10.1016/j.apsoil.2015.04.005
Andreas O Wagner ₁ , Nadine Praeg ₁ , Christoph Reitschuler ₁ , Paul Illmer ₁

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Highlights • We evaluated different soil DNA extraction procedures and disintegration strategies.• All tests were conducted using a reference soil.• We tested repeated DNA extractions (up to 10 times).• A method (EMA; PMA) for the discrimination of cells and free DNA was tested.• DNA yield is affected by extraction procedure, microbial diversity merely.

中文翻译：

DNA 提取程序、重复提取和单叠氮化乙锭 (EMA)/单叠氮化丙锭 (PMA) 处理对参考土壤中细菌、真菌和古细菌的总体 DNA 产量和微生物指纹图谱的影响

亮点 • 我们评估了不同的土壤 DNA 提取程序和分解策略。• 所有测试均使用参考土壤进行。• 我们测试了重复的 DNA 提取（最多 10 次）。• 用于区分细胞和测试了游离 DNA。• DNA 产量仅受提取程序、微生物多样性的影响。

更新日期：2015-09-01

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