Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

中文翻译：

---

DU-145细胞中大小依赖性细胞毒性和商业胶体金纳米颗粒的摄取。

尿路感染（UTI）是前列腺癌患者的主要疾病。大肠杆菌ORN178（EC-178）是一种尿毒症，通过与前列腺癌细胞（DU-145细胞）的粘附素特异性结合而引起反复感染。金纳米颗粒（GNP）已用于病原体的生物诊断。在这项研究中，我们研究了EC-178与DU-145细胞的结合时间，细胞毒性以及普通和甘露糖功能化的20和200 nm GNP（d-甘露聚糖（Mn）-GNP）的摄取。我们还研究了与胎牛血清一起孵育时GNP的蛋白电晕，以研究决定GNP生物学命运的蛋白电晕。观察到EC-178在潜伏期3小时内结合并在DU-145细胞内。在DU-145细胞的对数和滞后阶段，普通的20 nm GNP会在48和72 h内降低存活细胞的百分比。还观察到，DU-145细胞吸收的Mn-GNP明显多于普通GNP。当GNPs与胎牛血清一起孵育时，观察到蛋白电晕，这通过动态光散射测量和SDS-PAGE凝胶得到证实。