Isolation of the lipid fraction from biological samples has been a crucial part of countless studies over the last century. This considerable research interest has led to the development of a number of methods for isolating a range of molecular species that fall under the umbrella term “lipid”. Such methods vary in popularity, complexity, specificity and even toxicity. In this review, we explore examples of published methods (1952–2014) for isolating lipids from biological samples and attempt to assess the limits of techniques both from a chemical and biological perspective. We also suggest how a suitable method might be chosen for a novel application.

中文翻译：

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从生物样品中分离脂质。

从生物样品中分离脂质部分是上个世纪无数研究的关键部分。这种巨大的研究兴趣导致开发了许多方法来分离属于“脂质”总称的一系列分子种类。这样的方法在流行性，复杂性，特异性甚至毒性方面有所不同。在本综述中，我们探索了从生物样品中分离脂质的已公开方法（1952-2014）的示例，并尝试从化学和生物学角度评估技术的局限性。我们还建议如何为新颖的应用选择合适的方法。