Photodynamic therapy (PDT) is a promising treatment methodology whereby diseased cells and tissues are destroyed by reactive oxygen species (ROS) by using a combination of light and photosensitizers (PS). The medical application of Rose Bengal (RB), photosensitizer with very good ROS generation capability, is limited due to its intrinsic toxicity and insufficient lipophilicity. In this report, we evaluate the potential of polyamidoamine (PAMAM) dendrimers in delivering RB and its phototoxic efficiency towards a model cancer cell line. The spherical, nanoscaled dendrimers could efficiently encapsulate RB and showed characteristic spectral responses. The controlled release property of dendrimer–RB formulation was clearly evident from the in vitro drug release study. ROS generation was confirmed in dendrimer–RB system upon white light illumination. Photosensitization of Dalton’s Lymphoma Ascite (DLA) cells incubated with dendrimer–RB formulation caused remarkable photocytotoxicity. Importantly, the use of dendrimer-based delivery system reduced the dark toxicity of RB.

中文翻译：

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使用纳米结构的聚（酰胺基胺）树状聚合物增强玫瑰孟加拉的光动力学功效和有效递送：在癌症的光动力学治疗中的潜在应用。

光动力疗法（PDT）是一种很有前途的治疗方法，通过结合使用光敏剂（PS）和活性氧（ROS）可以破坏患病的细胞和组织。由于其固有的毒性和不充分的亲脂性，Rose Bengal（RB）具有很好的ROS生成能力的光敏剂在医学上的应用受到了限制。在本报告中，我们评估了聚酰胺胺（PAMAM）树状聚合物在传递RB中的潜力及其对模型癌细胞系的光毒性效率。球形的纳米级树状聚合物可以有效地包封RB，并显示出特征光谱响应。从体外药物释放研究可以清楚地看出树状聚合物-RB制剂的控释特性。在白光照射下，在树状大分子-RB体系中证实了ROS的产生。用树状大分子-RB制剂孵育的道尔顿淋巴瘤腹水（DLA）细胞的光敏性引起了显着的光细胞毒性。重要的是，基于树状聚合物的递送系统的使用降低了RB的黑暗毒性。