The present study discusses evaluation of pullulan-functionalized doxorubicin nanoparticles for asialoglycoprotein receptor-mediated uptake in the Hep G2 cell line. Doxorubicin hydrochloride (DOX) nanoparticles using polymers of different hydrophobic character, polyethylene sebacate (hydrophobic) and poly (lactic-co-glycolic acid) (intermediate hydrophobicity) with high entrapment efficiency and particle size were prepared by modified nanoprecipitation, using Gantrez AN 119 as complexing agent. Nanoparticles of Gantrez AN 119 were also prepared to represent a hydrophilic polymer. Cell uptake of DOX nanoparticles was found to be comparable to DOX solution irrespective of DOX concentration, nanoparticles size, and pullulan concentration. Furthermore, uptake of nanoparticles functionalized with or without pullulan prepared with polymers of different hydrophobic character revealed comparable uptake. Comparable uptake of DOX solution and DOX nanoparticles functionalized with or without pullulan suggest extracellular release of DOX as the mechanism of uptake from the nanoparticles. In vivo evaluation in hepatic cancer model is therefore essential to confirm the role of pullulan as asialoglycoprotein receptors ligand.

中文翻译：

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评估支链淀粉功能化的阿霉素纳米颗粒对Hep G2细胞系中去唾液酸糖蛋白受体介导的摄取的影响。

本研究讨论了支链淀粉功能化的阿霉素纳米颗粒对Hep G2细胞系中去唾液酸糖蛋白受体介导的摄取的评估。采用改进的纳米沉淀法，使用Gantrez AN 119作为改性纳米沉淀法，制备了具有不同疏水特性的聚合物，疏水性高的聚癸二酸聚乙烯酯和中等疏水性的聚乳酸-乙醇酸共聚物（DOX）纳米粒。络合剂。还制备了Gantrez AN 119的纳米颗粒以代表亲水性聚合物。发现DOX纳米颗粒的细胞摄取与DOX溶液相当，而与DOX浓度，纳米颗粒大小和支链淀粉浓度无关。此外，用或不用支链淀粉而用不同疏水特性的聚合物制备的功能化的纳米颗粒的摄取显示出可比的摄取。有或没有支链淀粉功能化的DOX溶液和DOX纳米颗粒的可比吸收表明，DOX的细胞外释放是从纳米颗粒吸收的机制。因此，在肝癌模型中进行体内评估对于确认支链淀粉作为去唾液酸糖蛋白受体配体的作用至关重要。