Structural and cellular attachment analysis identified overall bent helical regions of adhesive peptides identified within mussel adhesive protein (MAP) capable of also attaching cells. DOPA (L-DOPA, 3,4-dihydroxyphenylalanine) is frequently identified and credited for the attachment ability of several marine proteins. Newly designed cyclic peptides (DOPA-G-G-C-G-K-A-K-G-C [cyc-DOPA] & Y-G-G-C-G-K-A-K-G-C [cyc-Y]) derived from structurally conserved regions of several MAP peptides were examined to assist in the understanding of both surface and cellular attachment. Solution-state proton nuclear magnetic resonance (NMR) spectroscopy coupled with molecular modeling and dynamics revealed minimal differences in the structures of the proposed cellular attachment domain within these two peptides. Multiple attenuated internal reflection infrared (MAIR-IR) spectroscopy, ellipsometry, and advancing contact angle analyses showed that formation of thin films by these peptides was L-DOPA- and pH-dependent. When compared with control surfaces, undifferentiated leukocyte cells (MOLT-4) significantly attached and spread onto films created from the cyc-DOPA. The culmination of these structural, biophysical, and cellular attachment techniques reveal a conformation of cyc-DOPA that is capable of both adsorbing to surfaces and then attaching cells that spread. This work supports the sequence K-A-K as the cellular attachment domain, especially when held in a reliable structural conformation.

中文翻译：

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具有细胞附着能力的循环生物粘合剂的结构和生物物理表征

结构和细胞附着分析确定了在贻贝粘附蛋白 (MAP) 内鉴定的粘附肽的整体弯曲螺旋区域，也能够粘附细胞。多巴 (L-DOPA, 3,4-dihydroxyphenylalanine) 经常被鉴定并被认为是几种海洋蛋白质的附着能力。新设计的环肽（DOPA-GGCGKAKGC [cyc-DOPA] 和 YGGCGKAKGC [cyc-Y]）来源于几种 MAP 肽的结构保守区域，以帮助理解表面和细胞附着。溶液态质子核磁共振 (NMR) 光谱与分子建模和动力学相结合，揭示了这两种肽内提议的细胞附着域结构的最小差异。多重衰减内反射红外 (MAIR-IR) 光谱、椭圆光度法和先进的接触角分析表明，这些肽形成的薄膜依赖于 L-DOPA 和 pH。与对照表面相比，未分化的白细胞 (MOLT-4) 显着附着并扩散到由 cyc-DOPA 制成的薄膜上。这些结构、生物物理和细胞附着技术的高潮揭示了 cyc-DOPA 的构象，它能够吸附到表面，然后附着扩散的细胞。这项工作支持序列 KAK 作为细胞附着域，特别是当保持在可靠的结构构象中时。与对照表面相比，未分化的白细胞 (MOLT-4) 显着附着并扩散到由 cyc-DOPA 制成的薄膜上。这些结构、生物物理和细胞附着技术的高潮揭示了 cyc-DOPA 的构象，它能够吸附到表面，然后附着扩散的细胞。这项工作支持序列 KAK 作为细胞附着域，特别是当保持在可靠的结构构象中时。与对照表面相比，未分化的白细胞 (MOLT-4) 显着附着并扩散到由 cyc-DOPA 制成的薄膜上。这些结构、生物物理和细胞附着技术的高潮揭示了 cyc-DOPA 的构象，它能够吸附到表面，然后附着扩散的细胞。这项工作支持序列 KAK 作为细胞附着域，特别是当保持在可靠的结构构象中时。