A simple and reliable method for fabricating single-use normally closed burst valves in thermoplastic microfluidic devices is presented, using a process flow that is readily integrated into established workflows for the fabrication of thermoplastic microfluidics. An experimental study of valve performance reveals the relationships between valve geometry and burst pressure. The technology is demonstrated in a device employing multiple valves engineered to actuate at different inlet pressures that can be generated using integrated screw pumps. On-chip storage and reconstitution of fluorescein salt sealed within defined reagent chambers are demonstrated. By taking advantage of the low gas and water permeability of cyclic olefin copolymer, the robust burst valves allow on-chip hermetic storage of reagents, making the technology well suited for the development of integrated and disposable assays for use at the point of care.

中文翻译：

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一次性使用的热塑性微流体爆破阀可实现片上试剂存储。

提出了一种简单而可靠的方法，该方法使用易于集成到已建立的用于制造热塑性微流体的工作流程中的工艺流程来制造热塑性微流体设备中的一次性使用的常闭爆破阀。阀门性能的实验研究揭示了阀门几何形状与爆破压力之间的关系。这项技术在采用多个阀门的设备中得到了证明，该阀门经过工程设计，可以使用集成的螺杆泵在不同的入口压力下进行致动。展示了密封在规定的试剂室内的荧光素盐在芯片上的存储和重构。通过利用环状烯烃共聚物的低气体和水渗透性，坚固的爆破阀可以在芯片上进行试剂的气密存储，