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Plasma exosomal miR-30a-5p inhibits osteogenic differentiation of bone marrow mesenchymal stem cells from a chronic unpredictable mild stress-induced depression rat model Mol. Cell. Probes (IF 3.3) Pub Date : 2024-03-23 Boyu Tan, Xueyao Jiang, Li Chen, Rongsheng Wang, Hongyan Wei
With rising society stress, depression-induced osteoporosis is increasing. However, the mechanism involved is unclear. In this study, we explored the effect of plasma exosomal miRNAs on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation in a chronic unpredictable mild stress (CUMS)-induced depression rat model. After 12 weeks of CUMS-induced depression, the pathological changes in
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Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria Mol. Cell. Probes (IF 3.3) Pub Date : 2024-03-19 Kristen Haggerty, Stuart Cantlay, Emily Young, Mariah K. Cashbaugh, Elio F. Delatore III, Rori Schreiber, Hayden Hess, Daniel R. Komlosi, Sarah Butler, Dalton Bolon, Theresa Evangelista, Takoda Hager, Claire Kelly, Katherine Phillips, Jada Voellinger, Robert M.Q. Shanks, Joseph Horzempa
Utilization of fluorescent proteins is widespread for the study of microbial pathogenesis and host-pathogen interactions. Here, we discovered that linkage of the 36 N-terminal amino acids of FTL_0580 (a hypothetical protein of ) to fluorescent proteins increases the fluorescence emission of bacteria that express these recombinant fusions. This N-terminal peptide will be referred to as 580N. Western
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Impact of miRNAs in the pathoetiology of recurrent implantation failure Mol. Cell. Probes (IF 3.3) Pub Date : 2024-03-13 Mohadeseh Fathi, Mohammad Amin Omrani, Sepideh Kadkhoda, Akram Ghahghaei-Nezamabadi, Soudeh Ghafouri-Fard
Recurrent implantation failure (RIF) is a condition with a multifactorial basis. Recent research has focused on the role of genetic factors in the pathophysiology of RIF. Of particular note, miRNAs have been found to contribute to the pathogenesis of RIF. Several miRNA polymorphisms have been investigated in this context. Moreover, dysregulation of expression of a number of miRNAs, including miR-374a-5p
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Decrease of exosomal miR-21-5p and the increase of CD62p+ exosomes are associated with the development of sepsis in polytraumatized patients Mol. Cell. Probes (IF 3.3) Pub Date : 2024-03-08 Birte Weber, Dirk Henrich, Ingo Marzi, Liudmila Leppik
Sepsis as a severe systemic inflammation leads oftentimes to organ dysfunction and subsequently to death. In polytrauma patients, septic complications represent with 45% the predominant cause of late death and are responsible for extremely high costs in the healthcare system. Therefore, clinicians have to detect as early as possible the begin of sepsis to improve the patient's outcome. One new promising
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Reference data on estrogen metabolome in healthy pregnancy Mol. Cell. Probes (IF 3.3) Pub Date : 2024-03-04 Gellért Karvaly, Krisztián Kovács, Marcell Gyarmatig, Dóra Gerszi, Sándor Nagy, Dlovan Ali Jalal, Zoltán Tóth, Barna Vasarhelyi, Béla Gyarmati
Estrogen hormones and their metabolites are implicated in the maintenance of healthy pregnancy and adequate fetal development. Abnormal levels were related to increased risk of pregnancy complications, particularly preeclampsia. Our aims were (1) to develop a methodological platform for the comprehensive assessment of estrogen metabolome in pregnancy; (2) to collect healthy reference data for relevant
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Evaluation of diagnostic potential of CD38 in rickets Mol. Cell. Probes (IF 3.3) Pub Date : 2024-01-25 Yongjie Xia, Xiaoshuo Ye, Wei Chen, Chao You, Chao Deng, Yibiao Zhou
Background Rickets occurs in infants and children (aged 2 months to 3 years), compromising their skeletal development and damaging nervous, hematopoietic, immune, and other system functions. This study aimed to explore the significance of CD38 in rickets. Methods The microarray dataset GSE22523 was analyzed to obtain differentially expressed genes in rickets patients. A total of 36 rickets patients
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Intrahepatic cholangiocarcinoma biomarkers: Towards early detection and personalized pharmacological treatments Mol. Cell. Probes (IF 3.3) Pub Date : 2024-01-20 Maurizio Capuozzo, Mariachiara Santorsola, Francesco Ferrara, Claudia Cinque, Stefania Farace, Renato Patrone, Vincenza Granata, Andrea Zovi, Guglielmo Nasti, Alessandro Ottaiano
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Platelet-derived extracellular vesicles are associated with kidney injury in patients with urosepsis Mol. Cell. Probes (IF 3.3) Pub Date : 2024-01-13 Zepeng Zhu, Xiaofeng Zhu, Dong Wang, Xun Lu, Tiancheng Jiang, Lei Zhang, Ming Chen, Aibing Yao, Shuqiu Chen
Background There is increasing evidence that platelet-derived extracellular vesicles (PEVs) may be involved in the mechanisms of inflammatory storm and organ damage in sepsis. However, there are no available studies on PEVs and renal injury in patients with urosepsis. Methods We analyzed the concentration and ratio of PEVs in plasma by flow cytometry and measured plasma IL-1β/IL-6/TNF-α/NGAL levels
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Recombinant Slit2 attenuates tracheal fibroblast activation in benign central airway obstruction by inhibiting the TGF-β1/Smad3 signaling pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-12-22 Chunyan He, Lei Gu, Anmao Li, Yishi Li, Rui Xiao, Jiaxin Liao, Junhao Mu, Yiling Gan, Mingyu Peng, Giri Mohan, Wei Liu, Li Xu, Shuliang Guo
Airway fibrosis is among the pathological manifestations of benign central airway obstruction noted in the absence of effective treatments and requires new drug targets to be developed. Slit guidance ligand 2–roundabout guidance receptor 1 (Slit2–Robo1) is involved in fibrosis and organ development. However, its significance in airway fibrosis has not yet been reported. The study explored how the recombinant
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Pancancer analysis uncovers an immunological role and prognostic value of the m6A reader IGF2BP2 in pancreatic cancer Mol. Cell. Probes (IF 3.3) Pub Date : 2023-12-22 Hui Deng, Hanming Yao, Shurui Zhou, Chong He, Yuzhou Huang, Yunlong Li, Hanwei Chen, Jianchang Shu
Introduction Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant gastrointestinal tumors worldwide with a dismal prognosis and high relapse rate. PDAC is considered a “cold cancer” for which immunotherapy is not effective. Therefore, to improve the prognosis for PDAC patients, it is urgent to explore the mechanism driving its insensitivity to immunotherapy. Materials and methods We
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Real-time single-base specific detection of the Haemonchus contortus S168T variant associated with levamisole resistance using loop-primer endonuclease cleavage loop-mediated isothermal amplification Mol. Cell. Probes (IF 3.3) Pub Date : 2023-12-19 Alistair Antonopoulos, Owen Higgins, Stephen R. Doyle, David Bartley, Alison Morrison, Maha Mansour Shalaby, Julien Reboud, Eileen Devaney, Terry J. Smith, Roz Laing, Valentina Busin
Haemonchus contortus is a parasitic haematophagous nematode that primarily affects small ruminants and causes significant economic loss to the global livestock industry. Treatment of haemonchosis typically relies on broad-spectrum anthelmintics, resistance to which is an important cause of treatment failure. Resistance to levamisole remains less widespread than to other major anthelmintic classes,
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USP5: Comprehensive insights into structure, function, biological and disease-related implications, and emerging therapeutic opportunities Mol. Cell. Probes (IF 3.3) Pub Date : 2023-12-04 Si-Ting Gao, Xin Xin, Zhuo-yuan Wang, Yi-yang Hu, Qin Feng
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MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1 Mol. Cell. Probes (IF 3.3) Pub Date : 2023-12-03 Hui Liu, Zengfeng Ni, Lili Shi, Lijie Ma, Jianqiang Zhao
Abstract not available
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Prenatal and postnatal genetic testing toward personalized care: The non-invasive perinatal testing Mol. Cell. Probes (IF 3.3) Pub Date : 2023-11-16 Lilla Botos, Erzsébet Szatmári, Gyula Richárd Nagy
This article investigates how non-invasive prenatal testing and the incorporation of genomic sequencing into newborn screening postnatally are transforming perinatal care. They improve the accuracy of prenatal and neonatal screening, allowing for early interventions and personalized therapies. Non-invasive prenatal testing before birth and saliva-sample-based newborn genomic sequencing after birth
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First trimester prediction models for small-for- gestational age and fetal growth restricted fetuses without the presence of preeclampsia Mol. Cell. Probes (IF 3.3) Pub Date : 2023-11-16 Ilona Hromadnikova, Katerina Kotlabova, Ladislav Krofta
We established efficient first trimester prediction models for small-for-gestational age (SGA) and fetal growth restriction (FGR) without the presence of preeclampsia (PE) regardless of the gestational age of the onset of the disease [early FGR occurring before 32 gestational week or late FGR occurring after 32 gestational week]. The retrospective study was performed on singleton Caucasian pregnancies
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Transcription factor TEAD4 facilitates glycolysis and proliferation of gastric cancer cells by activating PKMYT1 Mol. Cell. Probes (IF 3.3) Pub Date : 2023-11-02 Lifen Zhan, Wen Wu, Qiongling Yang, Huiqun Shen, Limin Liu, Renzhi Kang
Background Gastric cancer (GC) ranks third for cancer deaths worldwide, and glycolysis is a hallmark of several cancers, including GC. TEAD4 plays a role in establishing an oncogenic cascade in cancers, including GC. Whether TEAD4 can influence the glycolysis of GC cells remains uncovered. Hence, this study attempted to investigate the impact on glycolysis of GC cells by TEAD4. Methods By using bioinformatics
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KIF2C promotes clear cell renal cell carcinoma progression via activating JAK2/STAT3 signaling pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-28 Hao Deng, Xiaobo Gong, Guanghai Ji, Chenglong Li, Shaoping Cheng
Background Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors that can be highly aggressive. Despite advances in the exploration of its underlying molecular biology, the clinical outcome for advanced ccRCC is still unsatisfied. Recently, more attention was paid to the functions of Kinesin family member 2C (KIF2C) in cancer progression, while the specific function of
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AKAP12 inhibits esophageal squamous carcinoma cell proliferation, migration, and cell cycle via the PI3K/AKT signaling pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-28 Xingyi Li, Hao Dong, Yifan Zheng, Shengguang Ding, Yan Li, Hefei Li, HaiTao Huang, Congjun Zhong, Tian Xie, Yiming Xu
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Screening protective miRNAs and constructing novel lncRNAs/miRNAs/mRNAs networks and prognostic models for triple-negative breast cancer Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-24 Yuelei Zhao, Yichen Song, Yan Zhang, Meiju Ji, Peng Hou, Fang Sui
Triple-negative breast cancer (TNBC) represents 10–20 % of all breast cancer (BC) cases and is characterized by poor prognosis. Given the urgent need to improve prognostication and develop specific therapies for TNBC, the identification of new molecular targets is of great importance. MicroRNA (miRNA) has been reported as a valuable and novel molecular target in the progression of TNBC. However, the
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Long noncoding RNA RMRP ameliorates doxorubicin-induced apoptosis by interacting with PFN1 in a P53-Dependent manner Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-13 Juexing Li, Lei Zhou, Yuanliang Jiang, Hailan Gao, Tuersuntuoheti Maierhaba, Hui Gong
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Cancer-associated fibroblasts secret extracellular vesicles to support cell proliferation and epithelial-mesenchymal transition in laryngeal squamous cell carcinoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-09 Tingting Li, Linli Tian, Jing Cao, Ming Liu
As the critical components of tumor microenvironment, cancer-associated fibroblasts (CAFs) support the development of various type of cancers, including laryngeal squamous cell carcinoma (LSCC), but the detailed molecular mechanisms by which cancer-associated fibroblasts interact with LSCC cells to facilitate its progression have not been fully uncovered. In the present study, by analyzing the contents
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LINC02253 promote the malignant phenotype of Colon adenocarcinoma cells by up-regulating WWP1-mediated SMAD3 ubiquitination Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-09 Jinfeng Wu, Xianhong Lu, Jinzhong Yu, Pan Li, Xiqiu Yu
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Identification of hub genes and potential inhibitory compounds in the process of liver transplantation through transcriptome sequencing Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-07 Chujun Duan, Xiaojun Zhao, Xiao Li, Jiangang Xie, Yi Si, Linxiao Wang, Dan Wu, Yifan Wang, Shanshou Liu, Qianmei Wang, Ran Zhuang, Wen Yin, Junjie Li
Liver transplantation (LT) is the best choice for patients with end-stage liver diseases. In order to better understand pathophysiological alterations in LT, we aimed to identify potential hub genes and inhibitory compounds involved in the LT process. Four pairs of peripheral blood mononuclear cell (PBMC) samples of the LT recipients before and after surgery were collected and taken for transcriptome
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Non-coding RNA profile for natural killer cell activity Mol. Cell. Probes (IF 3.3) Pub Date : 2023-10-08 Soudeh Ghafouri-Fard, Arian Askari, Alireza Zangooie, Hamed Shoorei, Hasan Pourmoshtagh, Mohammad Taheri
Natural killer cells (NK cells) are a type of cytotoxic lymphocytes which are involved in innate immunity, alongside with assisting with adaptive immune response. Since they have cytotoxic effects, disruptions in their functionality and development leads to a variety of conditions, whether malignant or non-malignant. The profile and interaction of these non-coding RNAs and NK cells in different conditions
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CD3ζ as a novel predictive biomarker of PD-1 inhibitor resistance in melanoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-09-28 Zhuo Zhang, Duoli Zhang, Fang Wang, Jiao Liu, Xian Jiang, Songyot Anuchapreeda, Singkome Tima, Zhangang Xiao, Suwit Duangmano
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Exploring the role of PMEPA1 in gastric cancer Mol. Cell. Probes (IF 3.3) Pub Date : 2023-09-25 Fei Wen, Shangyu Yang, WeiWen Cai, Mengyuan Zhao, Long Qin, Zuoyi Jiao
Although there are several treatments available for gastric cancer (GC), the prognosis of the disease is still poor due to many factors, such as late diagnosis and tumor heterogeneity. To identify potential therapeutic targets, bioinformatics techniques and clinical sample validation were employed and prostate transmembrane protein androgen induced 1 (PMEPA1) was selected for further study. In the
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Interrelationship of hemoglobin A1c level lipid profile, uric acid, C-reactive protein levels and age in a large hospital database Mol. Cell. Probes (IF 3.3) Pub Date : 2023-09-20 Dlovan Ali Jalal, Barna Vásárhelyi, Béla Blaha, Zoltán Tóth, Tamás Géza Szabó, Béla Gyarmati
Introduction Hemoglobin A1c (HbA1c) is used to monitor glucose homeostasis and to identify risk for diabetes. As diabetic patients are frequently present with dyslipidaemia, low-grade inflammation and hyperuricemia, we tested whether HbA1c levels can be estimated having the information about lipid profile, uric acid (UA) and C-reactive protein (CRP) levels. We developed formulas to describe the association
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Current landscape of miRNAs and TGF‐β signaling in lung cancer progression and therapeutic targets Mol. Cell. Probes (IF 3.3) Pub Date : 2023-09-15 Bashdar Mahmud Hussen, Safeen Jasim Saleem, Snur Rasool Abdullah, Sayran Mohamadtahr, Hazha Jamal Hidayat, Mohammed Fatih Rasul, Mohammad Taheri, Arda Kiani
Lung cancer (LC) is the primary reason for cancer-associated fatalities globally. Due to both tumor-suppressing and tumor-promoting activities, the TGF-β family of growth factors is extremely essential to tumorigenesis. A non-coding single-stranded short RNA called microRNA (miRNA), which is made up of about 22 nt and is encoded by endogenous genes, can control normal and pathological pathways in various
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Introduced the ITGB1-DT as a novel biomarker associated with five potential drugs using bioinformatics analysis of breast cancer proteomics data and RT-PCR. Mol. Cell. Probes (IF 3.3) Pub Date : 2023-09-11 Zahra Yousefian Naeini,Negin Esfandiari,Mehrdad Hashemi,Kiavash Hushmandi,Sedighe Arbabian,Maliheh Entezari
BACKGROUND Breast cancer (BC) has been identified as a significant contributor to the rising number of female cancer deaths. As, it has become clear that breast cancer development depends on the interplay of several biological factors against a single molecule. This research aimed to use proteomics to gain a regulatory and metabolic understanding of BC pathophysiology. METHOD For the study, a breast
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16S full-length gene sequencing analysis of intestinal flora in breast cancer patients in Hainan Province Mol. Cell. Probes (IF 3.3) Pub Date : 2023-08-29 Yonglan Jiang, Wei Gong, Zhenyong Xian, Weihua Xu, Junjie Hu, Zhichao Ma, Huaying Dong, Chong Lin, Shengmiao Fu, Xinping Chen
Breast cancer has become the number one cancer in the world, and intestinal flora may be closely linked to it. Geographic location also has an important impact on human intestinal flora. We conducted the first study on the intestinal flora of breast cancer patients and non-breast cancer patients in a tropical region - Hainan Province in China. At the same time, Pacbio platform based on third-generation
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A network pharmacology-based approach to explore the effect of dihydromyricetin on non-alcoholic fatty liver rats via regulating PPARG and CASP3 Mol. Cell. Probes (IF 3.3) Pub Date : 2023-08-22 Lu Liu, Sen Sun, Xiaohua Li
Background Non-alcohol fatty liver disease (NAFLD) is the most prevalent hepatopathy in China, with few effective cures currently. This work aimed to confirm the effect of DHM in vivo/vitro and explore the potential mechanism based on a network pharmacology-based approach. Methods The rats were fed using a high-fat diet (HFD) to accumulate lipid. DHM at different concentrations was used to treat the
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Blood exosome marker miRNA-30d-5p: Role and regulation mechanism in cell stemness and gemcitabine resistance of hepatocellular carcinoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-08-10 Biao Tang, Longhui Xie, Xin Tang, Junjie Tian, Shaofei Xiao
Background Cancer stem cells (CSCs) are different from regular cancer cells because of their self-renewal feature and differentiation potential, which establishes the backbone of the vital role of CSCs in the progress and drug resistance of hepatocellular carcinoma (HCC). The objective of this study was to evaluate the effects of blood exosome-derived miRNA-30d-5p on the stemness and gemcitabine resistance
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CDK12 loss inhibits cell proliferation by regulating TBK1 in non-small cell lung cancer cells Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-29 Xiaoli Liu, Yangdong Liu, Wenjun Chai, Mingxia Yan, Hui Li, Jing Li, Lei Sun, Yue Cao, Qian Liu, Yuexi Sun, Hongyu Pan
Lung cancer is one of the most common malignant tumors and has a poor prognosis and a low survival rate. Traditional treatments, such as radiotherapy and chemotherapy, still face some challenges because of high drug resistance and toxicity. Therefore, it is necessary to discover a new kind of targeted drug with low toxicity and high efficiency. CDK12 is a cell cycle-dependent kinase whose main function
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CAPG interference induces apoptosis and ferroptosis in colorectal cancer cells through the P53 pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-30 Yingying Zhao, Rui Ma, Chuyue Wang, Rong Hu, Weili Wu, Xiang Sun, Baotao Chen, Wen Zhang, You Chen, Jiajian Zhou, Ping Yuan
Purpose Given the high incidence and mortality rates of colorectal cancer (CRC) and the inadequacy of existing treatments for many patients, this study aimed to explore the potential of Capping Actin Protein (CAPG), a protein involved in actin-related movements, as a novel therapeutic target for CRC. Methods Bioinformatic analysis of gene expression was conducted using the UALCAN website. Cell proliferation
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DNMT1-mediated DNA methylation in toll-like receptor 4 (TLR4) inactivates NF-κB signal pathway-triggered pyroptotic cell death and cellular inflammation to ameliorate lipopolysaccharides (LPS)-induced osteomyelitis Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-31 Muguo Song, Junyi Li, Jian Sun, Xiaoyong Yang, Xijiao Zhang, Kehan Lv, Yongqing Xu, Jian Shi
Toll-like receptor 4 (TLR4) plays a critical role in various human diseases, and was associated with pyroptotic cell death and inflammatory responses. DNA methylation, which has stable and reversible properties, has been reported to alter the expression of target genes, including TLR4. However, the role of methylated TLR4 in osteomyelitis (OM) and the underlying molecular mechanisms remain unclear
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Formin-related protein 1 facilitates proliferation and aggressive phenotype of clear cell renal cell carcinoma through MAPK/MMP2 pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-19
Background Formin-related protein-1(FRL1) has reportedly been overexpressed in a variety of malignancies, such as clear cell renal cell carcinoma (ccRCC). However, the clinical value and molecular mechanisms underlying ccRCC tumorigenesis and progression in association with FRL1 remain poorly understood. Methods Immunohistochemical analysis was performed on 119 paraffin-embedded RCC tissue samples
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COTE-1 promotes the proliferation and invasion of small cell lung cancer by regulating autophagy activity via the AMPK/mTOR signaling pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-18
Background COTE-1 has been found to promote the proliferation and invasion of non-small cell lung cancer. However, the mechanism of COTE-1 in SCLC is still unclear. Exploring the role of COTE-1 in SCLC is expected to provide a potential target for the prognosis and treatment of SCLC. Methods The expression of COTE-1 and ki-67 was detected by immunohistochemical staining. PCR detected COTE-1 expression
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CircCPSF6 promotes hepatocellular carcinoma cancer progression by regulating MAP4K4 through sponging miR-145-5p Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-13
Background Aberrant expression of circRNAs is involved in the progression of hepatocellular carcinoma (HCC). This study aimed at screening the pro-tumorigenic circular RNAs (circRNAs) in HCC and the mechanisms of circCPSF6 expression influencing HCC characteristics. Method circCPSF6 was identified in HCC tissues using high-throughput sequencing data, and its expression was verified in both HCC tissues
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Evaluation of the expression level of some coding and non-coding genes in oral squamous cell carcinoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-07-01 Mohadeseh Ajorlou, Parisa Bina-Jourshari, Sepideh Mirzaei, Mazaher Maghsoudloo, Mehrdad Hashemi, Neda Mousavi-Niri, Maliheh Entezari
Introduction Oral squamous cell carcinoma (OSCC) is the most common cancers arising from the head and neck region. There is growing evidence that lncRNAs play an important role in OSCC progression. The study aims to investigate correlations between the expression levels of LncRNAs of PARROT, MYCNUT, DANCR, and KTN1-AS1 with clinicopathological characteristics and finding suitable biomarkers for OSCC
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Venous thromboembolism-related genetic determinant F11 rs4253417 is a potential prognostic factor in ischaemic stroke Mol. Cell. Probes (IF 3.3) Pub Date : 2023-06-27 Valéria Tavares, Joana Assis, Ricardo Pinto, Margarida Freitas-Silva, Rui Medeiros
Ischaemic stroke (IS) and venous thromboembolism (VTE) are two forms of thromboembolism that, although distinct, seem to share numerous risk factors. Concerning genetic risk factors, while many VTE genetic markers have been reported, inclusively by genome-wide association studies (GWAS), the identification and validation of genetic determinants underlying IS pathogenesis have been challenging. Considering
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Exploring the role of FBXO5 in gastric cancer Mol. Cell. Probes (IF 3.3) Pub Date : 2023-05-05 Junchang Zhang, Gengyuan Zhang, Keshen Wang, Feng Cui, Hanteng Yang, Zuoyi Jiao
Gastric cancer is one of the most common lethal malignancies in the world, especially in China. Due to the ineffective screening of early gastric cancer and drug resistance of the advanced, the prognosis of gastric cancer remains dismal. Based on bioinformatics and tissue microarray analyses, FBXO5 was selected for analysis in this study. Here, we report the function of FBXO5 in gastric cancer, showing
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Small peptide LINC00511-133aa encoded by LINC00511 regulates breast cancer cell invasion and stemness through the Wnt/β-catenin pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-04-27 Zhongqiu Tan, Lifeng Zhao, Shiqing Huang, Qiulan Jiang, Yantao Wei, Junyun Long Wu, Zhiwen Zhang, Yepeng Li
LINC00511 is an long non-coding RNA (lncRNA) of ncRNAs,This study aimed to investigate whether the lncRNA LINC00511 could encode a small peptide, LINC00511-133aa, and whether this peptide could promote breast cancer cell metastasis and stemness by activating the wnt/β-catenin pathway. The LINC00511-133aa coding sequence vector and control vector were transfected into MCF-7 and MDA-MB-231 breast cancer
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Evaluation of the immune checkpoint factors in idiopathic membranous nephropathy Mol. Cell. Probes (IF 3.3) Pub Date : 2023-04-21 Roza Motavalli, Maryam Hosseini, Mohammad Sadegh Soltani-Zangbar, Abbas Karimi, Mohammadreza Sadeghi, Sanam Dolati, Mehdi Yousefi, Jalal Etemadi
Idiopathic membranous nephropathy (IMN), a single-organ autoimmune disease, is recognized by autoantibodies to podocyte proteins and identified as the most frequent cause of nephrotic syndrome in adults. T cells are important contributors in autoimmunity since they promote B–cell development, antibody production, direct inflammation, and organ tissue cytotoxicity. This study investigated the inhibitory
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Variable fragment length allele-specific polymerase chain reaction (VFLASP), a method for simple and reliable genotyping Mol. Cell. Probes (IF 3.3) Pub Date : 2023-04-10 Tamás Tóth, Ákos Csaba, Attila Bokor, Nándor Ács
Single-nucleotide polymorphism (SNP) is a substitution of a single nucleotide at a specific position in the genome. Until now, 585 million SNPs have been identified in the human genome, and therefore, a widely applicable method is desirable to detect a specific SNP. Herein we report a simple and reliable genotyping assay, which seems to be suitable for medium and small size laboratories, as well, to
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Significance of microRNA-targeted ErbB signaling pathway genes in cardiomyocyte differentiation Mol. Cell. Probes (IF 3.3) Pub Date : 2023-04-05 Akram Gholipour, Ali Zahedmehr, Farshad Shakerian, Shiva Irani, Maziar Oveisee, Seyed Javad Mowla, Mahshid Malakootian
Objective(s) Cardiomyocyte differentiation is a complex process that follows the progression of gene expression alterations. The ErbB signaling pathway is necessary for various stages of cardiac development. We aimed to identify potential microRNAs targeting the ErbB signaling pathway genes by in silico approaches. Methods Small RNA-sequencing data were obtained from GSE108021 for cardiomyocyte differentiation
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TRAF7 inhibits glycolysis to potentiate growth inhibition and apoptosis of myeloid leukemia cells via regulating the KLF2-PFKFB3 axis Mol. Cell. Probes (IF 3.3) Pub Date : 2023-04-05 Lin Zou, Ye Fang, Wei He
Tumor necrosis factor receptor-related factor 7 (TRAF7) can regulate cell differentiation and apoptosis, but its specific functional mechanism in the pathological process of acute myeloid leukemia (AML) closely related to differentiation and apoptosis disorders is largely unclear. In this study, TRAF7 was found to be lowly expressed in AML patients and a variety of myeloid leukemia cells. TRAF7 was
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Potential of metagenomic next-generation sequencing in detecting infections of ICU patients Mol. Cell. Probes (IF 3.3) Pub Date : 2023-03-16 Yanxu Liang, Qingguo Feng, Kai Wei, Xiaoming Hou, Xiaotao Song, Yuantao Li
Background Due to the limitations of traditional microbiological detection techniques in evaluating complicated infections in ICU patients, it is necessary to explore novel and effective methods to improve the clinical detection of ICU patients’ infections. Objective This study aimed to evaluate the efficiency and specificity of mNGS in screening pathogens in the blood, deep phlegm, urine, and other
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LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression Mol. Cell. Probes (IF 3.3) Pub Date : 2023-03-16 Kexin Sun, Tingting Lu, Cheng Hu, Zhengyi Li, Jie Zhu, Li Zhang, Xiaotong Shao, Wei Chen
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DNA in fresh urine supernatant is not affected by additional centrifugation and is protected against deoxyribonuclease Mol. Cell. Probes (IF 3.3) Pub Date : 2023-02-18 Ľubica Janovičová, Katarína Kmeťová, Ľubomíra Tóthová, Barbora Vlková, Peter Celec
Urinary DNA is widely studied as a non-invasive marker for monitoring of kidneys after transplantation or the progression of urinary tract tumors. The quantity of urinary DNA especially of mitochondrial origin has been reported to mirror kidney damage in various renal diseases and their models. Processing of samples might affect urinary DNA concentrations but the details are not clear. Samples of urine
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Identification of miR-1-3p, miR-143–3p and miR-145–5p association with bone metastasis of Gleason 3+4 prostate cancer and involvement of LASP1 regulation Mol. Cell. Probes (IF 3.3) Pub Date : 2023-02-18 Hongwei Guo, Jinlong Zhao, Xinjun Li, Feifei Sun, Yiming Qin, Xiaorong Yang, Xueting Xiong, Qianshuo Yin, Xueli Wang, Lin Gao, Meng Jiao, Jing Hu, Bo Han
Gleason Score (GS) 3 + 4 prostate cancer (PCa) is heterogeneous in clinical course and molecular features. Risk stratification of indolent and aggressive PCa with GS 3 + 4 is critical, especially those with bone metastasis (BM) potential. Microarray-based microRNA(miRNA) profiling with eight PCa cases with or without BM was used to screen the candidate miRNAs associated with BM. Transwell and MTS assays
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Dexmedetomidine attenuates myocardial ischemia-reperfusion injury via inhibiting ferroptosis by the cAMP/PKA/CREB pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-02-14 Xiaojing Ma, Jia Xu, Nan Gao, Jun Tian, Tieying Song
This study is to investigate the effects of dexmedetomidine on myocardial ischemia-reperfusion (I/R) injury and its molecular mechanisms. H9c2 cell injury model was constructed by the hypoxia/normoxia (H/R) conditions. Besides, cAMP response element-binding protein (CREB) overexpression and knockdown cell lines were constructed. Cell viability was determined by cell-counting kit 8. Biochemical assays
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Identification of methyltransferase modification genes associated with prognosis and immune features of pancreatic adenocarcinoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-02-10 Wentao Wang, Dongyuan Zhang, Donglei Chang, Yupeng Li, Lei Ren
Background Pancreatic adenocarcinoma (PAAD) is a malignant tumor with a high mortality rate. Methylation modifications acted a crucial role to affect cancer progression. The current study aimed to explore the potential role of methylase regulators in PAAD prognosis and immune microenvironment. Methods PubMed and TCGA databases were used to systematically analyze methylase regulators in PAAD. We identified
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Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway Mol. Cell. Probes (IF 3.3) Pub Date : 2023-02-02
Prostaglandins participate in maternal recognition of pregnancy, implantation and maintenance of gestation. Prostaglandin transporter (PGT), as a candidate molecule of prostaglandin carriers, might be involved in the pathogenesis of preeclampsia. In preeclampsia (PE) patients’ placental tissue, we identified PGT by RNA sequencing, measured its expression pattern by quantitative real-time PCR and Western
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Extracellular vesicles derived from dental mesenchymal stem/stromal cells with gemcitabine as a cargo have an inhibitory effect on the growth of pancreatic carcinoma cell lines in vitro Mol. Cell. Probes (IF 3.3) Pub Date : 2023-01-25 Daniela Klimova, Jana Jakubechova, Ursula Altanerova, Andreas Nicodemou, Jakub Styk, Tomas Szemes, Vanda Repiska, Cestmir Altaner
Extracellular vesicles (EVs) are nowadays a target of interest in cancer therapy as a successful drug delivering tool. Based on their many beneficial biocompatible properties are designed to transport nucleic acids, proteins, various nanomaterials or chemotherapeutics. Extracellular vesicles derived from mesenchymal stem/stromal cells (MSCs) possess their tumor-homing abilities. This inspired us to
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Diagnostic and prognostic value of plasma cell-free DNA combined with VEGF-C in laryngeal squamous cell carcinoma Mol. Cell. Probes (IF 3.3) Pub Date : 2023-01-19 Qiang Huang, Mengyou Ji, Feiran Li, Yufeng Li, Xuehua Zhou, Chi-yao Hsueh, Liang Zhou
Background Circulating cell-free DNA (cfDNA) and vascular endothelial growth factor-C (VEGF-C) can be utilized to detect cancer and predict its prognosis. However, their potential application in laryngeal squamous cell carcinoma (LSCC) is unclear. Purpose This study aimed to identify the diagnostic and prognostic value of cfDNA and VEGF-C in LSCC patients. Methods The plasma cfDNA of 148 LSCC patients
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MiR-128-3p as blood based liquid biopsy biomarker in childhood acute lymphoblastic leukemia Mol. Cell. Probes (IF 3.3) Pub Date : 2023-01-11 Andrea Rzepiel, Anna Horváth, Nóra Kutszegi, András Gézsi, Judit C. Sági, Laura Almási, Bálint Egyed, Péter Lőrincz, Tamás Visnovitz, Gábor T. Kovács, Csaba Szalai, Ágnes F. Semsei, Dániel J. Erdélyi
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Liquid biopsy in the clinical management of cancer patients Mol. Cell. Probes (IF 3.3) Pub Date : 2023-01-04 Orsolya Biró
Abstract not available
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Clonal diversity in KRAS mutant colorectal adenocarcinoma under treatment: Monitoring of cfDNA using reverse hybridization and DNA sequencing platforms Mol. Cell. Probes (IF 3.3) Pub Date : 2022-12-29 Emese Sarolta Bádon, Attila Mokánszki, Anikó Mónus, Csilla András, Gábor Méhes
Biological heterogeneity is a key feature of malignancies that significantly contributes to disease progression and therapy resistance. Residual/relapsed tumor foci may represent genetically divergent subclones, which remain uncovered as repeated and multiple tumor sampling is usually limited. The analysis of circulating free DNA (cfDNA) from the peripheral blood plasma (also called a liquid biopsy
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Development of a specific and sensitive diagnostic PCR for rapid molecular authentication of the medicinal plant Portulaca oleracea Mol. Cell. Probes (IF 3.3) Pub Date : 2022-12-26 Mo-Rong Xu, Meng-Shiou Lee, Bo-Cheng Yang, Hsiu-Chi Chang, Chao-Lin Kuo, Chia-Hsin Lin, Hsi-Jien Chen, Jai-Hong Cheng, Fang-Chun Sun
Adulteration by Bacopa monnieri (BM) in Portulaca oleracea (PO) plants frequently occurs; it decreases the efficacy of traditional Chinese medicine (TCM) and leads to fraud in the herbal marketplace. In this study, a diagnostic PCR assay was established for the rapid authentication of PO and BM in the herbal market. The sequence divergences in internal transcribed spacer 2 (ITS2) between PO and its
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Identification of liquid biopsy-based mutations in colorectal cancer by targeted sequencing assays Mol. Cell. Probes (IF 3.3) Pub Date : 2022-12-10 István Szász, Tímea Kiss, Attila Mokánszki, Viktória Koroknai, János Deák, Vikas Patel, Krisztina Jámbor, Róza Ádány, Margit Balázs
Recently, liquid biopsy, as a promising approach was introduced for the analysis of different tumor-derived circulating markers including tumor DNA and cell free DNA (ct/cfDNA). Identification of mutations in cfDNA may allow the early detection of tumors, as well as predicting and monitoring treatment responses in a minimally invasive way. In the present study, we used commercially available gene panels