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3D bioprinting approaches for spinal cord injury repair Biofabrication (IF 9.0) Pub Date : 2024-04-22 Jingwei Jiu, Haifeng Liu, Dijun Li, Jiarong Li, Lu Liu, Wenjie Yang, Lei Yan, Songyan Li, Jing Zhang, Xiaoke Li, Jiao Jiao Li, Bin Wang
Regenerative healing of spinal cord injury (SCI) poses an ongoing medical challenge by causing persistent neurological impairment and a significant socioeconomic burden. The complexity of spinal cord tissue presents hurdles to successful regeneration following injury, due to the difficulty of forming a biomimetic structure that faithfully replicates native tissue using conventional tissue engineering
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The use of microphysiological systems to model metastatic cancer Biofabrication (IF 9.0) Pub Date : 2024-04-18 Caitlin E Jackson, Nicola H Green, William R English, Frederik Claeyssens
Cancer is one of the leading causes of death in the 21st century, with metastasis of cancer attributing to 90% of cancer-related deaths. Therefore, to improve patient outcomes there is a need for better preclinical models to increase the success of translating oncological therapies into the clinic. Current traditional static in vitro models lack a perfusable network which is critical to overcome the
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3D printing processes in precise drug delivery for personalized medicine Biofabrication (IF 9.0) Pub Date : 2024-04-17 Haisheng Peng, Bo Han, Tianjian Tong, Xin Jin, Yanbo Peng, Meitong Guo, Bian Li, Jiaxin Ding, Qingfei Kong, Qun Wang
With the advent of personalized medicine, the drug delivery system will be changed significantly. The development of personalized medicine needs the support of many technologies, among which three-dimensional printing (3DP) technology is a novel formulation-preparing process that creates 3D objects by depositing printing materials layer-by-layer based on the computer-aided design method. Compared with
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Steering cell orientation through light-based spatiotemporal modulation of the mechanical environment Biofabrication (IF 9.0) Pub Date : 2024-04-16 Ignasi Jorba, Sil Gussenhoven, Atze van der Pol, Bart GW Groenen, Maarten van Zon, Marie José Goumans, Nicholas A Kurniawan, Tommaso Ristori, Carlijn VC Bouten
The anisotropic organization of cells and the extracellular matrix (ECM) is essential for the physiological function of numerous biological tissues, including the myocardium. This organization changes gradually in space and time, during disease progression such as myocardial infarction. The role of mechanical stimuli has been demonstrated to be essential in obtaining, maintaining and de-railing this
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Using a micro-device with a deformable ceiling to probe stiffness heterogeneities within 3D cell aggregates Biofabrication (IF 9.0) Pub Date : 2024-04-15 Shreyansh Jain, Hiba Belkadi, Arthur Michaut, Sébastien Sart, Jérôme Gros, Martin Genet, Charles N Baroud
Recent advances in the field of mechanobiology have led to the development of methods to characterise single-cell or monolayer mechanical properties and link them to their functional behaviour. However, there remains a strong need to establish this link for three-dimensional (3D) multicellular aggregates, which better mimic tissue function. Here we present a platform to actuate and observe many such
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Bioprinting of human dermal microtissues precursors as building blocks for endogenous in vitro connective tissue manufacturing Biofabrication (IF 9.0) Pub Date : 2024-04-15 Annachiara Scalzone, Giorgia Imparato, Francesco Urciuolo, Paolo A Netti
The advent of 3D bioprinting technologies in tissue engineering has unlocked the potential to fabricate in vitro tissue models, overcoming the constraints associated with the shape limitations of preformed scaffolds. However, achieving an accurate mimicry of complex tissue microenvironments, encompassing cellular and biochemical components, and orchestrating their supramolecular assembly to form hierarchical
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Wet 3D printing of biodegradable porous scaffolds to enable room-temperature deposition modeling of polymeric solutions for regeneration of articular cartilage Biofabrication (IF 9.0) Pub Date : 2024-04-12 Xiaoye Yu, Peng Wang, Jingming Gao, Ye Fu, Qunsong Wang, Jun Chen, Shiyi Chen, Jiandong Ding
Tissue engineering has emerged as an advanced strategy to regenerate various tissues using different raw materials, and thus it is desired to develop more approaches to fabricate tissue engineering scaffolds to fit specific yet very useful raw materials such as biodegradable aliphatic polyester like poly (lactide-co-glycolide) (PLGA). Herein, a technique of ‘wet 3D printing’ was developed based on
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Culture insert device with perfusable microchannels enhances in vitro skin model development and barrier function assessment Biofabrication (IF 9.0) Pub Date : 2024-04-12 Dina Mikimoto, Masahito Mori, Akemi Toyoda, Kazuyuki Yo, Haruka Oda, Shoji Takeuchi
The ever-stricter regulations on animal experiments in the field of cosmetic testing have prompted a surge in skin-related research with a special focus on recapitulation of the in vivo skin structure in vitro. In vitro human skin models are seen as an important tool for skin research, which in recent years attracted a lot of attention and effort, with researchers moving from the simplest 2-layered
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A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements Biofabrication (IF 9.0) Pub Date : 2024-04-12 Daniel Vera, María García-Díaz, Núria Torras, Óscar Castillo, Xavi Illa, Rosa Villa, Mar Alvarez, Elena Martinez
Conventional gut-on-chip (GOC) models typically represent the epithelial layer of the gut tissue, neglecting other important components such as the stromal compartment and the extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity and function. These models often employ hard, flat porous membranes for cell culture, thus failing to recapitulate the soft environment
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Versatile xanthan gum-based support bath material compatible with multiple crosslinking mechanisms: rheological properties, printability, and cytocompatibility study Biofabrication (IF 9.0) Pub Date : 2024-04-11 Guanyu Lai, Laurence Meagher
Extrusion-based bioprinting is a promising technology for the fabrication of complex three-dimensional (3D) tissue-engineered constructs. To further improve the printing accuracy and provide mechanical support during the printing process, hydrogel-based support bath materials have been developed. However, the gel structure of some support bath materials can be compromised when exposed to certain bioink
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Fabrication of a tailor-made conductive polyaniline/ascorbic acid-coated nanofibrous mat as a conductive and antioxidant cell-free cardiac patch Biofabrication (IF 9.0) Pub Date : 2024-04-09 Farzad Moradikhah, Iman Shabani, Mohammad Tafazzoli Shadpour
Polyaniline (PANI) was in-situ polymerized on nanofibrous polycaprolactone mats as cell-free antioxidant cardiac patches (CPs), providing electrical conductivity and antioxidant properties. The fabricated CPs took advantage of intrinsic and additive antioxidant properties in the presence of PANI backbone and ascorbic acid as a biocompatible dopant of PANI. The antioxidant nature of CPs may reduce the
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Advancing preclinical drug evaluation through automated 3D imaging for high-throughput screening with kidney organoids Biofabrication (IF 9.0) Pub Date : 2024-04-08 Haruka Oishi, Nahid Tabibzadeh, Ryuji Morizane
High-throughput drug screening is crucial for advancing healthcare through drug discovery. However, a significant limitation arises from available in vitro models using conventional 2D cell culture, which lack the proper phenotypes and architectures observed in three-dimensional (3D) tissues. Recent advancements in stem cell biology have facilitated the generation of organoids—3D tissue constructs
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3D bioprinting of stromal cells-laden artificial cornea based on visible light-crosslinkable bioinks forming multilength networks Biofabrication (IF 9.0) Pub Date : 2024-04-05 Gyeong Won Lee, Ajeesh Chandrasekharan, Subhadeep Roy, Akash Thamarappalli, Binapani Mahaling, Hyeseon Lee, Keum-Yong Seong, Sourabh Ghosh, Seung Yun Yang
3D bioprinting has the potential for the rapid and precise engineering of hydrogel constructs that can mimic the structural and optical complexity of a healthy cornea. However, the use of existing light-activated bioinks for corneal printing is limited by their poor cytocompatibility, use of cytotoxic photoinitiators (PIs), low photo-crosslinking efficiency, and opaque/colored surface of the printed
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Nuclear deformation and cell division of single cell on elongated micropatterned substrates fabricated by DMD lithography Biofabrication (IF 9.0) Pub Date : 2024-04-02 Duo Zhang, Wenjie Wu, Wanying Zhang, Qiyu Feng, Qingchuan Zhang and Haiyi Liang
Cells sense mechanical signals from the surrounding environment and transmit them to the nucleus through mechanotransduction to regulate cellular behavior. Microcontact printing, which utilizes elastomer stamps, is an effective method for simulating the cellular microenvironment and manipulating cell morphology. However, the conventional fabrication process of silicon masters and elastomer stamps requires
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Application of additively manufactured bone scaffold: a systematic review Biofabrication (IF 9.0) Pub Date : 2024-03-28 Qianyu Shi, Jibing Chen, Junsheng Chen, Yanfeng Liu and Hongze Wang
The application of additive manufacturing (AM) technology plays a significant role in various fields, incorporating a wide range of cutting-edge technologies such as aerospace, medical treatment, electronic information, and materials. It is currently widely adopted for medical services, national defense, and industrial manufacturing. In recent years, AM has also been extensively employed to produce
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Printing GelMA bioinks: a strategy for building in vitro model to study nanoparticle-based minocycline release and cellular protection under oxidative stress Biofabrication (IF 9.0) Pub Date : 2024-03-28 Zhouquan Fu, Nan Hai, Yinghui Zhong and Wei Sun
Owing to its thermoresponsive and photocrosslinking characteristics, gelatin methacryloyl (GelMA)-based biomaterials have gained widespread usage as a novel and promising bioink for three-dimensional bioprinting and diverse biomedical applications. However, the flow behaviors of GelMA during the sol-gel transition, which are dependent on time and temperature, present significant challenges in printing
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On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process Biofabrication (IF 9.0) Pub Date : 2024-03-28 Benjamin Reineke, Ilona Paulus, Sophia Löffelsend, Chien-Hsin Yu, Dmitrii Vinogradov, Anna Meyer, Jonas Hazur, Jonas Röder, Madita Vollmer, Gültekin Tamgüney, Stephan Hauschild, Aldo R Boccaccini, Jürgen Groll and Stephan Förster
Bioprinting has evolved into a thriving technology for the fabrication of cell-laden scaffolds. Bioinks are the most critical component for bioprinting. Recently, microgels have been introduced as a very promising bioink, enabling cell protection and the control of the cellular microenvironment. However, the fabrication of the bioinks involves the microfluidic production of the microgels, with a subsequent
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Development of a novel bioartificial cornea using 3D bioprinting based on electrospun micro-nanofibrous decellularized extracellular matrix Biofabrication (IF 9.0) Pub Date : 2024-03-28 Shi-yao Zhang, Mingshan Zhang, Xin-yu Li, Shuai Huang, Daobo Han, Le Chang, Liyun Ling, Yan Huo, Mohammad Alzogool, Ning Yang, Qing Ye and Yan Wang
Corneal damage contributes to blindness in millions of people. Simulating natural corneas with artificial corneas is challenging due to material and manufacturing limitations, including poor mechanical properties, complex manufacturing processes, and ocular histocompatibility. In this study, electrospun micro-nanofibrous decellularized extracellular matrix (dECM) is combined with digital light processing
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Dynamic, IPSC-derived hepatic tissue tri-culture system for the evaluation of liver physiology in vitro Biofabrication (IF 9.0) Pub Date : 2024-03-25 Benedikt Scheidecker, Stéphane Poulain, Masahiro Sugimoto, Taketomo Kido, Takumi Kawanishi, Atsushi Miyajima, Soo Hyeon Kim, Hiroshi Arakawa, Yukio Kato, Masaki Nishikawa, Mathieu Danoy, Yasuyuki Sakai and Eric Leclerc
Availability of hepatic tissue for the investigation of metabolic processes is severely limited. While primary hepatocytes or animal models are widely used in pharmacological applications, a change in methodology towards more sustainable and ethical assays is highly desirable. Stem cell derived hepatic cells are generally regarded as a viable alternative for the above model systems, if current limitations
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Polyhydroxybutyrate-based osteoinductive mineralized electrospun structures that mimic components and tissue interfaces of the osteon for bone tissue engineering Biofabrication (IF 9.0) Pub Date : 2024-03-25 M Sriram, Smriti Priya and Dhirendra S Katti
Scaffolds for bone tissue engineering should enable regeneration of bone tissues with its native hierarchically organized extracellular matrix (ECM) and multiple tissue interfaces. To achieve this, inspired by the structure and properties of bone osteon, we fabricated polyhydroxybutyrate (PHB)-based mineralized electrospun fibrous scaffolds. After studying multiple PHB-based fibers, we chose 7%PHB/1%Gelatin
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Generating human skeletal myoblast spheroids for vascular myogenic tissue engineering Biofabrication (IF 9.0) Pub Date : 2024-03-21 Mendy Minne, Lisanne Terrie, Rebecca Wüst, Steffie Hasevoets, Kato Vanden Kerchove, Kakra Nimako, Ivo Lambrichts, Lieven Thorrez and Heidi Declercq
Engineered myogenic microtissues derived from human skeletal myoblasts offer unique opportunities for varying skeletal muscle tissue engineering applications, such as in vitro drug-testing and disease modelling. However, more complex models require the incorporation of vascular structures, which remains to be challenging. In this study, myogenic spheroids were generated using a high-throughput, non-adhesive
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Microfluidic-based preparation of artificial antigen-presenting gel droplets for integrated and minimalistic adoptive cell therapy strategies Biofabrication (IF 9.0) Pub Date : 2024-03-15 Yishen Tian, Wei Chen, Guangshi Du, Jie Gao, Youbo Zhao, Zhuli Wang, Min Su, Rong Hu, Feng Han
Adoptive T-cell transfer for cancer therapy is limited by the inefficiency of in vitro T-cell expansion and the ability of in vivo T-cells to infiltrate tumors. The construction of multifunctional artificial antigen-presenting cells is a promising but challenging approach to achieve this goal. In this study, a multifunctional artificial antigen-presenting gel droplet (AAPGD) was designed. Its surface
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Decellularized tissue exhibits large differences of extracellular matrix properties dependent on decellularization method: novel insights from a standardized characterization on skeletal muscle Biofabrication (IF 9.0) Pub Date : 2024-03-15 Lisanne Terrie, Charlot Philips, Ewout Muylle, Antoine Weisrock, Pauline Lecomte-Grosbras, Lieven Thorrez
Decellularized matrices are an attractive choice of scaffold in regenerative medicine as they can provide the necessary extracellular matrix (ECM) components, signals and mechanical properties. Various detergent-based protocols have already been proposed for decellularization of skeletal muscle tissue. However, a proper comparison is difficult due to differences in species, muscle origin and sample
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Bioreactor-based stem cell therapy for liver fibrosis Biofabrication (IF 9.0) Pub Date : 2024-03-14 Mengchao Yan, Jia Yao, Ye Xie, Pan Jiang, Jun Yan, Xun Li
Stem cell therapy, achieved using mesenchymal stem cells (MSCs), has been highlighted for the treatment of liver fibrosis. Infusion into the circulatory system is a traditional application of MSCs; however, this approach is limited by phenotypic drift, stem cell senescence, and vascular embolism. Maintaining the therapeutic phenotype of MSCs while avoiding adverse infusion-related reactions is the
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Sheet-based extrusion bioprinting: a new multi-material paradigm providing mid-extrusion micropatterning control for microvascular applications Biofabrication (IF 9.0) Pub Date : 2024-03-14 Ryan Hooper, Caleb Cummings, Anna Beck, Javier Vazquez-Armendariz, Ciro Rodriguez, David Dean
As bioprinting advances into clinical relevance with patient-specific tissue and organ constructs, it must be capable of multi-material fabrication at high resolutions to accurately mimick the complex tissue structures found in the body. One of the most fundamental structures to regenerative medicine is microvasculature. Its continuous hierarchical branching vessel networks bridge surgically manipulatable
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Engineering pre-vascularized 3D tissue and rapid vascular integration with host blood vessels via co-cultured spheroids-laden hydrogel Biofabrication (IF 9.0) Pub Date : 2024-03-14 Hyunseok Kwon, Sangmin Lee, Hayeon Byun, Seung Jae Huh, Eunjin Lee, Eunhyung Kim, Jinkyu Lee, Heungsoo Shin
Recent advances in regenerative medicine and tissue engineering have enabled the biofabrication of three-dimensional (3D) tissue analogues with the potential for use in transplants and disease modeling. However, the practical use of these biomimetic tissues has been hindered by the challenge posed by reconstructing anatomical-scale micro-vasculature tissues. In this study, we suggest that co-cultured
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Fixation method of a single muscle fiber by magnetic force for stretching, transportation, and evaluation of mechanical properties Biofabrication (IF 9.0) Pub Date : 2024-03-14 Zhaoyu Wang, Taisuke Masuda, Fumihito Arai
Engineered muscle fibers are attracting interest in bio-actuator research as they can contribute to the fabrication of actuators with a high power/size ratio for micro-robots. These fibers require to be stretched during culture for functional regulation as actuators and require a fixation on a rigid substrate for stretching in culture and evaluation of mechanical properties, such as Young’s modulus
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Surface slicing and toolpath planning for in-situ bioprinting of skin implants Biofabrication (IF 9.0) Pub Date : 2024-03-14 Muhammad Salman Chaudhry, Aleksander Czekanski
Bioprinting has emerged as a successful method for fabricating engineered tissue implants, offering great potential for wound healing applications. This study focuses on an advanced surface-based slicing approach aimed at designing a skin implant specifically for in-situ bioprinting. The slicing step plays a crucial role in determining the layering arrangement of the tissue during printing. By utilizing
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3D bioprinting of mouse pre-osteoblasts and human MSCs using bioinks consisting of gelatin and decellularized bone particles Biofabrication (IF 9.0) Pub Date : 2024-03-13 Aylin Kara Özenler, Thomas Distler, Ashwini Rahul Akkineni, Funda Tihminlioglu, Michael Gelinsky, Aldo R Boccaccini
One of the key challenges in biofabrication applications is to obtain bioinks that provide a balance between printability, shape fidelity, cell viability, and tissue maturation. Decellularization methods allow the extraction of natural extracellular matrix, preserving tissue-specific matrix proteins. However, the critical challenge in bone decellularization is to preserve both organic (collagen, proteoglycans)
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Bioassembly of hemoglobin-loaded photopolymerizable spheroids alleviates hypoxia-induced cell death Biofabrication (IF 9.0) Pub Date : 2024-03-12 Axel E Norberg, Ezgi Bakirci, Khoon S Lim, Paul D Dalton, Tim B F Woodfield, Gabriella C J Lindberg
The delivery of oxygen within tissue engineered constructs is essential for cell survivability; however, achieving this within larger biofabricated constructs poses a significant challenge. Efforts to overcome this limitation often involve the delivery of synthetic oxygen generating compounds. The application of some of these compounds is problematic for the biofabrication of living tissues due to
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Current advances in engineering meniscal tissues: insights into 3D printing, injectable hydrogels and physical stimulation based strategies Biofabrication (IF 9.0) Pub Date : 2024-03-08 Ashutosh Bandyopadhyay, Baishali Ghibhela, Biman B Mandal
The knee meniscus is the cushioning fibro-cartilage tissue present in between the femoral condyles and tibial plateau of the knee joint. It is largely avascular in nature and suffers from a wide range of tears and injuries caused by accidents, trauma, active lifestyle of the populace and old age of individuals. Healing of the meniscus is especially difficult due to its avascularity and hence requires
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Antibacterial, ROS scavenging and angiogenesis promoting ϵ-Polylysine/gelatin based hydrogel containing CTLP to regulate macrophages for pressure ulcer healing Biofabrication (IF 9.0) Pub Date : 2024-03-07 PinLi Lin, Xiaona Tang, Jintao Zhong, Fang Tang, Hanjiao Liu, Lu Peng, Biyu Wan, Mengya Wang, Yuling Ye, Rui Guo, Xusheng Liu, Lili Deng
Pressure ulcers (PUs) have emerged as a substantial burden on individuals and society. The introduction of innovative dressings that facilitate the healing of pressure ulcer wounds represents a cost-effective alternative for treatment. In this study, the emphasis is on the preparation of Carthamus tinctorius L. polysaccharide (CTLP) as hydrogel microspheres (MPs), which are then encapsulated within
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Three dimensional (bio)printing of blood vessels: from vascularized tissues to functional arteries Biofabrication (IF 9.0) Pub Date : 2024-03-07 Shubham Makode, Satyajit Maurya, Seyed A Niknam, Evelyn Mollocana-Lara, Kiana Jaberi, Negar Faramarzi, Ali Tamayol, Mehdi Mortazavi
Tissue engineering has emerged as a strategy for producing functional tissues and organs to treat diseases and injuries. Many chronic conditions directly or indirectly affect normal blood vessel functioning, necessary for material exchange and transport through the body and within tissue-engineered constructs. The interest in vascular tissue engineering is due to two reasons: (1) functional grafts
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Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation Biofabrication (IF 9.0) Pub Date : 2024-03-07 Ana Paula Pereira Guimaraes, Italo Rodrigo Calori, Roberta Sessa Stilhano, Antonio Claudio Tedesco
‘On-a-chip’ technology advances the development of physiologically relevant organ-mimicking architecture by integrating human cells into three-dimensional microfluidic devices. This method also establishes discrete functional units, faciliting focused research on specific organ components. In this study, we detail the development and assessment of a convoluted renal proximal tubule-on-a-chip (PT-on-a-chip)
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Artificial tumor matrices and bioengineered tools for tumoroid generation Biofabrication (IF 9.0) Pub Date : 2024-03-06 Yung-Chiang Liu, Ping Chen, Ray Chang, Xingjian Liu, Jhe-Wei Jhang, Myagmartsend Enkhbat, Shan Chen, Hongxia Wang, Chuxia Deng, Peng-Yuan Wang
The tumor microenvironment (TME) is critical for tumor growth and metastasis. The TME contains cancer-associated cells, tumor matrix, and tumor secretory factors. The fabrication of artificial tumors, so-called tumoroids, is of great significance for the understanding of tumorigenesis and clinical cancer therapy. The assembly of multiple tumor cells and matrix components through interdisciplinary techniques
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Fabrication of gradient hydrogels using a thermophoretic approach in microfluidics Biofabrication (IF 9.0) Pub Date : 2024-03-04 Alexandros Kosmidis Papadimitriou, Shin Wei Chong, Yi Shen, Oisin Stefan Lee, Tuomas P J Knowles, Liam M Grover, Daniele Vigolo
The extracellular matrix presents spatially varying physical cues that can influence cell behavior in many processes. Physical gradients within hydrogels that mimic the heterogenous mechanical microenvironment are useful to study the impact of these cues on cellular responses. Therefore, simple and reliable techniques to create such gradient hydrogels are highly desirable. This work demonstrates the
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A well plate-based GelMA photo-crosslinking system with tunable hydrogel mechanical properties to regulate the PTH-mediated osteogenic fate Biofabrication (IF 9.0) Pub Date : 2024-02-29 Kyurim Paek, Sangwook Woo, Seung Jae Song, Min Kyeong Kim, Keewook Yi, Seok Chung, Jeong Ah Kim
Versatile and efficient regulation of the mechanical properties of the extracellular matrix is crucial not only for understanding the dynamic changes in biological systems, but also for obtaining precise and effective cellular responses in drug testing. In this study, we developed a well plate-based hydrogel photo-crosslinking system to effectively control the mechanical properties of hydrogels and
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3D modeling of normal skin and cutaneous squamous cell carcinoma. A comparative study in 2D cultures, spheroids, and 3D bioprinted systems Biofabrication (IF 9.0) Pub Date : 2024-02-29 Agata Kurzyk, Anna Szumera-Ciećkiewicz, Joanna Miłoszewska, Magdalena Chechlińska
The current cancer research and drug testing are primarily based on 2D cell cultures and animal models. However, these methods have limitations and yield distinct drug response patterns. This study addressed this gap by developing an innovative in vitro human three-dimensional (3D) normal skin model and a multicellular model of human cutaneous squamous cell carcinoma (cSCC) using 3D bioprinting technology
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A long-term storable gel-laden chip composite built in a multi-well plate enabling in situ cell encapsulation for high-throughput liver model Biofabrication (IF 9.0) Pub Date : 2024-02-23 Min Kyeong Kim, Jubin Park, Sungho Tak, Kyurim Paek, Geul Bang, Sang-Mi Woo, Naresh Kumar Ravichandran, Won Gi Hong, Hyun-Wook Kang, Hyang Kim, Ji Yong Bae, Jeong Ah Kim
Hydrogels are widely used as scaffold materials for constructing in vitro three-dimensional microphysiological systems. However, their high sensitivity to various external cues hinders the development of hydrogel-laden, microscale, and high-throughput chips. Here, we have developed a long-term storable gel-laden chip composite built in a multi-well plate, which enables in situ cell encapsulation and
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Biofabrication of biomimetic undulating microtopography at the dermal-epidermal junction and its effects on the growth and differentiation of epidermal cells Biofabrication (IF 9.0) Pub Date : 2024-02-22 Chuang Gao, Chunxiang Lu, Huazhen Liu, Yi Zhang, Hao Qiao, Aoxiang Jin, Qiqi Dai, Yuanyuan Liu
The undulating microtopography located at the junction of the dermis and epidermis of the native skin is called rete ridges (RRs), which plays an important role in enhancing keratinocyte function, improving skin structure and stability, and providing three-dimensional (3D) microenvironment for skin cells. Despite some progress in recent years, most currently designed and manufactured tissue-engineered
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Analysis of bioprinting strategies for skin diseases and injuries through structural and temporal dynamics: historical perspectives, research hotspots, and emerging trends Biofabrication (IF 9.0) Pub Date : 2024-02-22 Fei Teng, Wei Wang, Zhi-Qiang Wang, Gui-Xue Wang
This study endeavors to investigate the progression, research focal points, and budding trends in the realm of skin bioprinting over the past decade from a structural and temporal dynamics standpoint. Scholarly articles on skin bioprinting were obtained from WoSCC. A series of bibliometric tools comprising R software, CiteSpace, HistCite, and an alluvial generator were employed to discern historical
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Co-axial hydrogel spinning for facile biofabrication of prostate cancer-like 3D models Biofabrication (IF 9.0) Pub Date : 2024-02-20 Carlos F Guimarães, Shiqin Liu, Jie Wang, Emma Purcell, Tugba Ozedirne, Tanchen Ren, Merve Aslan, Qingqing Yin, Rui L Reis, Tanya Stoyanova, Utkan Demirci
Glandular cancers are amongst the most prevalent types of cancer, which can develop in many different organs, presenting challenges in their detection as well as high treatment variability and failure rates. For that purpose, anticancer drugs are commonly tested in cancer cell lines grown in 2D tissue culture on plastic dishes in vitro, or in animal models in vivo. However, 2D culture models diverge
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Cell viability prediction and optimization in extrusion-based bioprinting via neural network-based Bayesian optimization models Biofabrication (IF 9.0) Pub Date : 2024-02-15 Dorsa Mohammadrezaei, Lena Podina, Johanna De Silva, Mohammad Kohandel
The fields of regenerative medicine and cancer modeling have witnessed tremendous growth in the application of 3D bioprinting. Maintaining high cell viability throughout the bioprinting process is crucial for the success of this technology, as it directly affects the accuracy of the 3D bioprinted models, the validity of experimental results, and the discovery of new therapeutic approaches. Therefore
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Hydrodynamic shear stress’ impact on mammalian cell properties and its applications in 3D bioprinting Biofabrication (IF 9.0) Pub Date : 2024-02-15 Yani Zhang, Aidan O’Mahony, Yong He, Tracie Barber
As an effective cell assembly method, three-dimensional bioprinting has been widely used in building organ models and tissue repair over the past decade. However, different shear stresses induced throughout the entire printing process can cause complex impacts on cell integrity, including reducing cell viability, provoking morphological changes and altering cellular functionalities. The potential effects
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Directing ligament-mimetic bi-directional cell organization in scaffolds through zone-specific microarchitecture for ligament tissue engineering Biofabrication (IF 9.0) Pub Date : 2024-02-14 M Sriram, Smriti Priya, Aman Mahajan, Dhirendra S Katti
Ligament tissues exhibit zone-specific anisotropic cell organization. The cells in ligament-proper are longitudinally oriented, whereas, the cells in epiligament are circumferentially oriented. Therefore, scaffolds developed to regenerate ligament tissues should possess adequate architectural features to govern ligament-mimetic bi-directional cell organization. The scaffold architectural features along
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BMP-2-immobilized PCL 3D printing scaffold with a leaf-stacked structure as a physically and biologically activated bone graft Biofabrication (IF 9.0) Pub Date : 2024-02-09 Min Ji Kim, Jin-Ho Park, Ji Min Seok, Jiwoon Jung, Tae Sung Hwang, Hee-Chun Lee, Jin Ho Lee, Su A Park, June-Ho Byun, Se Heang Oh
Although three-dimensional (3D) printing techniques are used to mimic macro- and micro-structures as well as multi-structural human tissues in tissue engineering, efficient target tissue regeneration requires bioactive 3D printing scaffolds. In this study, we developed a bone morphogenetic protein-2 (BMP-2)-immobilized polycaprolactone (PCL) 3D printing scaffold with leaf-stacked structure (LSS) (3D-PLSS-BMP)
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4D printed shape-shifting biomaterials for tissue engineering and regenerative medicine applications Biofabrication (IF 9.0) Pub Date : 2024-02-09 Maria Kalogeropoulou, Pedro J Díaz-Payno, Mohammad J Mirzaali, Gerjo J V M van Osch, Lidy E Fratila-Apachitei, Amir A Zadpoor
The existing 3D printing methods exhibit certain fabrication-dependent limitations for printing curved constructs that are relevant for many tissues. Four-dimensional (4D) printing is an emerging technology that is expected to revolutionize the field of tissue engineering and regenerative medicine (TERM). 4D printing is based on 3D printing, featuring the introduction of time as the fourth dimension
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Cancer-mediated axonal guidance of sensory neurons in a microelectrode-based innervation MPS Biofabrication (IF 9.0) Pub Date : 2024-02-09 Matthijs van der Moolen, Andrea Lovera, Fulya Ersoy, Sacha Mommo, Peter Loskill, Paolo Cesare
Despite recent advances in the field of microphysiological systems (MPSs), availability of models capable of mimicking the interactions between the nervous system and innervated tissues is still limited. This represents a significant challenge in identifying the underlying processes of various pathological conditions, including neuropathic, cardiovascular and metabolic disorders. In this novel study
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UV laser-processed microstructure for building biohybrid actuators with anisotropic movement Biofabrication (IF 9.0) Pub Date : 2024-02-09 Hiroaki Mita, Yosuke Mizuno, Hiroto Tanaka, Toshinori Fujie
Fabrication of a biohybrid actuator requires muscle cells anisotropically aligned in a line, curve, or combination of lines and curves (similar to the microstructure of living muscle tissue) to replicate lifelike movements, in addition to considering the arrangement of skeletal structure or muscular structure with anisotropic straight patterns. Here, we report a UV laser-processed microstructure for
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Multilayer nanodrug delivery system with spatiotemporal drug release improves tumor microenvironment for synergistic anticancer therapy Biofabrication (IF 9.0) Pub Date : 2024-02-07 Jiahui Cai, Yibo Yang, Jia Zhang, Zhimin Bai, Xin Zhang, Kun Li, Ming Shi, Zhiwei Liu, Liming Gao, Jidong Wang, Jian Li
The inflammatory response is one of the general symptoms that accompany tumorigenesis, the pro-inflammatory factors cyclooxygenase-2 (COX-2) and COX-2-derived prostaglandin-2 (PGE-2) in the inflammatory environment surrounding tumors possess promoting tumor development, metastasis and angiogenesis effects. In addition, the hypoxic environment of tumors severely limits the effectiveness of photodynamic
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A microscale 3D organ on a chip for recapitulating reciprocal neuroendocrine crosstalk between the hypothalamus and the pituitary gland Biofabrication (IF 9.0) Pub Date : 2024-02-06 Se-Ra Park, Myung Geun Kook, Soo-Rim Kim, Jin Woo Lee, Young Soo Yu, Chan Hum Park, Soyi Lim, Byung-Chul Oh, YunJae Jung, In-Sun Hong
Conventional 2D or even recently developed 3D in vitro culture models for hypothalamus and pituitary gland cannot successfully recapitulate reciprocal neuroendocrine communications between these two pivotal neuroendocrine tissues known to play an essential role in controlling the body’s endocrine system, survival, and reproduction. In addition, most current vitro culture models for neuroendocrine tissues
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Enhancing quality control in bioprinting through machine learning Biofabrication (IF 9.0) Pub Date : 2024-02-02 Amedeo Franco Bonatti, Giovanni Vozzi, Carmelo De Maria
Bioprinting technologies have been extensively studied in literature to fabricate three-dimensional constructs for tissue engineering applications. However, very few examples are currently available on clinical trials using bioprinted products, due to a combination of technological challenges (i.e. difficulties in replicating the native tissue complexity, long printing times, limited choice of printable
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Cation-crosslinked κ-carrageenan sub-microgel medium for high-quality embedded bioprinting Biofabrication (IF 9.0) Pub Date : 2024-02-01 Hua Zhang, Yang Luo, Zeming Hu, Mengxi Chen, Shang Chen, Yudong Yao, Jie Yao, Xiaoqi Shao, Kerong Wu, Yabin Zhu, Jun Fu
Three-dimensional (3D) bioprinting embedded within a microgel bath has emerged as a promising strategy for creating intricate biomimetic scaffolds. However, it remains a great challenge to construct tissue-scale structures with high resolution by using embedded 3D bioprinting due to the large particle size and polydispersity of the microgel medium, as well as its limited cytocompatibility. To address
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Enhancement of lacrimal gland cell function by decellularized lacrimal gland derived hydrogel Biofabrication (IF 9.0) Pub Date : 2024-02-01 Katharina E Wiebe-Ben Zakour, Sema Kaya, Julia C Matros, Michael C Hacker, Amina Cheikh-Rouhou, Kristina Spaniol, Gerd Geerling, Joana Witt
Sustainable treatment of aqueous deficient dry eye (ADDE) represents an unmet medical need and therefore requires new curative and regenerative approaches based on appropriate in vitro models. Tissue specific hydrogels retain the individual biochemical composition of the extracellular matrix and thus promote the inherent cell´s physiological function. Hence, we created a decellularized lacrimal gland
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3D-printed bioink loading with stem cells and cellular vesicles for periodontitis-derived bone defect repair Biofabrication (IF 9.0) Pub Date : 2024-01-31 Guang-Tao Yu, Wen-Xiang Zhu, Yu-Yue Zhao, Hao Cui, Hao Chen, Yan Chen, Ting-Ting Ning, Ming-Deng Rong, Lang Rao, Dan-Dan Ma
The suitable microenvironment of bone regeneration is critically important for periodontitis-derived bone defect repair. Three major challenges in achieving a robust osteogenic reaction are the exist of oral inflammation, pathogenic bacteria invasion and unaffluent seed cells. Herein, a customizable and multifunctional 3D-printing module was designed with glycidyl methacrylate (GMA) modified epsilon-poly-L-lysine
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Bioassembly of multicellular spheroids to mimic complex tissue structure using surface-modified magnetized nanofibers Biofabrication (IF 9.0) Pub Date : 2024-01-22 Hayeon Byun, Sangmin Lee, Heungsoo Shin
Advancements in biofabrication have led to major strides toward creating authentic organ models; however, replicating intricate organ structures without scaffolds remains challenging. In this study, we introduce a method utilizing surface-modifiable magnetic nanofibers to achieve precise control over spheroid functions and geometrical features, allowing the creation of multiple functional domains within
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Development of hybrid 3D printing approach for fabrication of high-strength hydroxyapatite bioscaffold using FDM and DLP techniques Biofabrication (IF 9.0) Pub Date : 2024-01-16 Yu-Jui Cheng, Tsung-Han Wu, Yu-Sheng Tseng, Wen-Fan Chen
This study develops a hybrid 3D printing approach that combines fused deposition modeling (FDM) and digital light processing (DLP) techniques for fabricating bioscaffolds, enabling rapid mass production. The FDM technique fabricates outer molds, while DLP prints struts for creating penetrating channels. By combining these components, hydroxyapatite (HA) bioscaffolds with different channel sizes (600
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Ionically annealed zwitterionic microgels for bioprinting of cartilaginous constructs Biofabrication (IF 9.0) Pub Date : 2024-01-16 František Surman, Maryam Asadikorayem, Patrick Weber, Daniel Weber, Marcy Zenobi-Wong
Foreign body response (FBR) is a pervasive problem for biomaterials used in tissue engineering. Zwitterionic hydrogels have emerged as an effective solution to this problem, due to their ultra-low fouling properties, which enable them to effectively inhibit FBR in vivo. However, no versatile zwitterionic bioink that allows for high resolution extrusion bioprinting of tissue implants has thus far been
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Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate Biofabrication (IF 9.0) Pub Date : 2024-01-16 Prabha Acharya, Pranav Joshi, Sunil Shrestha, Na Young Choi, Sehoon Jeong, Moo-Yeal Lee
Human induced pluripotent stem cell (iPSC)-derived brain organoids have potential to recapitulate the earliest stages of brain development, serving as an effective in vitro model for studying both normal brain development and disorders. However, current brain organoid culture methods face several challenges, including low throughput, high variability in organoid generation, and time-consuming, multiple
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Proteolytic remodeling of 3D bioprinted tumor microenvironments Biofabrication (IF 9.0) Pub Date : 2024-01-12 Fatemeh Rasti Boroojeni, Sajjad Naeimipour, Philip Lifwergren, Annelie Abrahamsson, Charlotta Dabrosin, Robert Selegård, Daniel Aili
In native tissue, remodeling of the pericellular space is essential for cellular activities and is mediated by tightly regulated proteases. Protease activity is dysregulated in many diseases, including many forms of cancer. Increased proteolytic activity is directly linked to tumor invasion into stroma, metastasis, and angiogenesis as well as all other hallmarks of cancer. Here we show a strategy for