-
Engineering Streptomyces sp. CPCC 204095 for the targeted high-level production of isatropolone A by elucidating its pathway-specific regulatory mechanism Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-16 Cong Zhang, Qianqian Xu, Jie Fu, Linzhuan Wu, Yihong Li, Yuan Lu, Yuanyuan Shi, Hongmin Sun, Xingxing Li, Lifei Wang, Bin Hong
Isatropolone A and C, produced by Streptomyces sp. CPCC 204095, belong to an unusual class of non-benzenoid aromatic compounds and contain a rare seven-membered ring structure. Isatropolone A exhibits potent activity against Leishmania donovani, comparable to the only oral drug miltefosine. However, its variably low productivity represents a limitation for this lead compound in the future development
-
Optimizing microbioreactor cultivation strategies for Trichoderma reesei: from batch to fed-batch operations Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-15 Katja Rohr, Lisa Gremm, Bertram Geinitz, Etienne Jourdier, Wolfgang Wiechert, Fadhel Ben Chaabane, Marco Oldiges
Filamentous fungi have long been recognized for their exceptional enzyme production capabilities. Among these, Trichoderma reesei has emerged as a key producer of various industrially relevant enzymes and is particularly known for the production of cellulases. Despite the availability of advanced gene editing techniques for T. reesei, the cultivation and characterization of resulting strain libraries
-
Unlocking the genome of the non-sourdough Kazachstania humilis MAW1: insights into inhibitory factors and phenotypic properties Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-15 Damian Mielecki, Anna Detman, Tamara Aleksandrzak-Piekarczyk, Małgorzata Widomska, Aleksandra Chojnacka, Anna Stachurska-Skrodzka, Paulina Walczak, Elżbieta Grzesiuk, Anna Sikora
Ascomycetous budding yeasts are ubiquitous environmental microorganisms important in food production and medicine. Due to recent intensive genomic research, the taxonomy of yeast is becoming more organized based on the identification of monophyletic taxa. This includes genera important to humans, such as Kazachstania. Until now, Kazachstania humilis (previously Candida humilis) was regarded as a sourdough-specific
-
Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-12 Balder Werin, Wilhelm Hansson Wennersten, Robin Olsson, Oliwia Kołodziejczyk, Martin N. Andersson, Magnus Carlquist, Urban Johanson
The wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of repellents for insects, like the pine weevil (Hylobius abietis) feeding on conifer seedlings and causing damage in forestry. Heterologous expression of TRPA1 from pine weevil in the yeast Pichia pastoris can potentially provide protein for structural and functional
-
Green ecofriendly enhancement of cellulase productivity using agricultural wastes by Aspergillus terreus MN901491: statistical designs and detergent ability on cotton fabrics Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-12 Mohamed A.A. Abdella, Nehad E. Ahmed, Mohamed S. Hasanin
Cellulase is considered a group member of the hydrolytic enzymes, responsible for catalyzing the hydrolysis of cellulose and has various industrial applications. Agricultural wastes are used as an inexpensive source for several utilizable products throughout the world. So, searching for cellulase enzymes from fungal strains capable of utilizing agricultural wastes to increase productivity, reduce costs
-
Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-12 Li Zhang, Ling Tan, Meizi Liu, Yunhong Chen, Yu Yang, Yanfei Zhang, Guoping Zhao
Microbial cell surface display technology allows immobilizing proteins on the cell surface by fusing them to anchoring motifs, thereby endowing the cells with diverse functionalities. However, the assessment of successful protein display and the quantification of displayed proteins remain challenging. The green fluorescent protein (GFP) can be split into two non-fluorescent fragments, while they spontaneously
-
Deciphering antifungal and antibiofilm mechanisms of isobavachalcone against Cryptococcus neoformans through RNA-seq and functional analyses Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-12 Weidong Qian, Jiaxing Lu, Chang Gao, Qiming Liu, Yongdong Li, Qiao Zeng, Jian Zhang, Ting Wang, Si Chen
Cryptococcus neoformans has been designated as critical fungal pathogens by the World Health Organization, mainly due to limited treatment options and the prevalence of antifungal resistance. Consequently, the utilization of novel antifungal agents is crucial for the effective treatment of C. neoformans infections. This study exposed that the minimum inhibitory concentration (MIC) of isobavachalcone
-
Valorisation of cotton post-industrial textile waste into lactic acid: chemo-mechanical pretreatment, separate hydrolysis and fermentation using engineered yeast Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-10 Marta Simonetti, Pietro Butti, Raffaella Desiré Di Lorenzo, Valeria Mapelli, Paola Branduardi
The textile industry has several negative impacts, mainly because it is based on a linear business model that depletes natural resources and produces excessive amounts of waste. Globally, about 75% of textile waste is disposed of in landfills and only 25% is reused or recycled, while less than 1% is recycled back into new garments. In this study, we explored the valorisation of cotton fabric waste
-
Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc2 155 expressing mammalian steroidogenesis system Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-09 Mikhail Karpov, Nicolai Strizhov, Ludmila Novikova, Tatyana Lobastova, Sergey Khomutov, Andrei Shutov, Alexey Kazantsev, Marina Donova
Pregnenolone and progesterone are the life-important steroid hormones regulating essential vital functions in mammals, and widely used in different fields of medicine. Microbiological production of these compounds from sterols is based on the use of recombinant strains expressing the enzyme system cholesterol hydroxylase/C20-C22 lyase (CH/L) of mammalian steroidogenesis. However, the efficiency of
-
Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-09 Ririka Asama, Cher J. S. Liu, Masahiro Tominaga, Yu-Ru Cheng, Yasuyuki Nakamura, Akihiko Kondo, Hsiang-Yu Wang, Jun Ishii
Single-cell droplet microfluidics is an important platform for high-throughput analyses and screening because it provides an independent and compartmentalized microenvironment for reaction or cultivation by coencapsulating individual cells with various molecules in monodisperse microdroplets. In combination with microbial biosensors, this technology becomes a potent tool for the screening of mutant
-
Characterization of a pleiotropic regulator MtrA in Streptomyces avermitilis controlling avermectin production and morphological differentiation Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-08 Jinpin Tian, Yue Li, Chuanbo Zhang, Jianyu Su, Wenyu Lu
The macrolide antibiotic avermectin, a natural product derived from Streptomyces avermitilis, finds extensive applications in agriculture, animal husbandry and medicine. The mtrA (sav_5063) gene functions as a transcriptional regulator belonging to the OmpR family. As a pleiotropic regulator, mtrA not only influences the growth, development, and morphological differentiation of strains but also modulates
-
Bioenergy production from chicken feather waste by anaerobic digestion and bioelectrochemical systems Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-04 Dina Hassan El Salamony, Mohamed Salah Eldin Hassouna, Taha Ibrahim Zaghloul, Zhen He, Hanan Moustafa Abdallah
Poultry feather waste has a potential for bioenergy production because of its high protein content. This research explored the use of chicken feather hydrolysate for methane and hydrogen production via anaerobic digestion and bioelectrochemical systems, respectively. Solid state fermentation of chicken waste was conducted using a recombinant strain of Bacillus subtilis DB100 (p5.2). In the anaerobic
-
Transcriptomic profiling of an evolved Yarrowia lipolytica strain: tackling hexanoic acid fermentation to increase lipid production from short-chain fatty acids Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-03 Sergio Morales-Palomo, Clara Navarrete, José Luis Martínez, Cristina González-Fernández, Elia Tomás-Pejó
Short-chain fatty acids (SCFAs) are cost-effective carbon sources for an affordable production of lipids. Hexanoic acid, the acid with the longest carbon chain in the SCFAs pool, is produced in anaerobic fermentation of organic residues and its use is very challenging, even inhibiting oleaginous yeasts growth. In this investigation, an adaptive laboratory evolution (ALE) was performed to improve Yarrowia
-
Research advances in the identification of regulatory mechanisms of surfactin production by Bacillus: a review Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-02 Junqing Qiao, Rainer Borriss, Kai Sun, Rongsheng Zhang, Xijun Chen, Youzhou Liu, Yongfeng Liu
Surfactin is a cyclic hexalipopeptide compound, nonribosomal synthesized by representatives of the Bacillus subtilis species complex which includes B. subtilis group and its closely related species, such as B. subtilis subsp subtilis, B. subtilis subsp spizizenii, B. subtilis subsp inaquosorum, B. atrophaeus, B. amyloliquefaciens, B. velezensis (Steinke mSystems 6: e00057, 2021) It functions as a biosurfactant
-
Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-02 Marcel Albacar, Antonio Casamayor, Joaquín Ariño
The yeast Komagataella phaffii has become a very popular host for heterologous protein expression, very often based on the use of the AOX1 promoter, which becomes activated when cells are grown with methanol as a carbon source. However, the use of methanol in industrial settings is not devoid of problems, and therefore, the search for alternative expression methods has become a priority in the last
-
Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-01 Alexander Rill, Lei Zhao, Helge B. Bode
Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce a large number of specialized metabolites (natural products) encoded in biosynthetic gene clusters (BGC). Genetic tools for Photorhabdus and Xenorhabdus have been rare and applicable to only a few strains. In the
-
Transcriptomics aids in uncovering the metabolic shifts and molecular machinery of Schizochytrium limacinum during biotransformation of hydrophobic substrates to docosahexaenoic acid Microb. Cell Fact. (IF 6.4) Pub Date : 2024-04-01 Iqra Mariam, Eleni Krikigianni, Chloe Rantzos, Maurizio Bettiga, Paul Christakopoulos, Ulrika Rova, Leonidas Matsakas, Alok Patel
Biotransformation of waste oil into value-added nutraceuticals provides a sustainable strategy. Thraustochytrids are heterotrophic marine protists and promising producers of omega (ω) fatty acids. Although the metabolic routes for the assimilation of hydrophilic carbon substrates such as glucose are known for these microbes, the mechanisms employed for the conversion of hydrophobic substrates are not
-
“Metabolic burden” explained: stress symptoms and its related responses induced by (over)expression of (heterologous) proteins in Escherichia coli Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-30 Sofie Snoeck, Chiara Guidi, Marjan De Mey
Engineering bacterial strains to redirect the metabolism towards the production of a specific product has enabled the development of industrial biotechnology. However, rewiring the metabolism can have severe implications for a microorganism, rendering cells with stress symptoms such as a decreased growth rate, impaired protein synthesis, genetic instability and an aberrant cell size. On an industrial
-
Correction: Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-28 Ghada E. Hegazy, Nadia A. Soliman, Soha Farag, Ehab R. El-Helow, Hoda Y. Hassan, Yasser R. Abdel-Fattah
Correction: Microbial Cell Factories (2024) 23:20 https://doi.org/10.1186/s12934-023-02292-y In this article, the author name Hoda H. Yusef was incorrectly written as Hoda Y. Hassan. The last statement in the author contributions was incorrectly given as “YRA suggested the main point of his work” but should have been “YRA suggested the main point of this work”. The original article has been corrected
-
Regulation mechanism and bioactivity characteristic of surfactin homologues with C14 and C15 fatty acid chains Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-27 Yumeng Su, Ling Gao, Chenyu Li, Liang Wang, Huimin Zhou, Chenhao Zhang, Xiaole Xia
Surfactin, a green lipopeptide bio-surfactant, exhibits excellent surface, hemolytic, antibacterial, and emulsifying activities. However, a lack of clear understanding of the synthesis regulation mechanism of surfactin homologue components has hindered the customized production of surfactin products with different biological activities. In this study, exogenous valine and 2-methylbutyric acid supplementation
-
Non-ribosomal peptide synthetase (NRPS)-encoding products and their biosynthetic logics in Fusarium Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-27 Ziwei Huang, Wangjie Zhu, Yifan Bai, Xuelian Bai, Huawei Zhang
Fungal non-ribosomal peptide synthetase (NRPS)-encoding products play a paramount role in new drug discovery. Fusarium, one of the most common filamentous fungi, is well-known for its biosynthetic potential of NRPS-type compounds with diverse structural motifs and various biological properties. With the continuous improvement and extensive application of bioinformatic tools (e.g., anti-SMASH, NCBI
-
Green fabrication of ZnO nanoparticles via spirulina platensis and its efficiency against biofilm forming pathogens Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-27 Nashwa A. Ahmed, Amal S. Othman
Excessive consumption of antibiotics is considered one of the top public health threats, this necessitates the development of new compounds that can hamper the spread of infections. A facile green technology for the biosynthesis of Zinc oxide nanoparticles (ZnO NPs) using the methanol extract of Spirulina platensis as a reducing and stabilizing agent has been developed. A bunch of spectroscopic and
-
High cell density sequential batch fermentation for enhanced propionic acid production from glucose and glycerol/glucose mixture using Acidipropionibacterium acidipropionici Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-26 Tarek Dishisha, Mridul Jain, Rajni Hatti-Kaul
Propionic acid fermentation from renewable feedstock suffers from low volumetric productivity and final product concentration, which limits the industrial feasibility of the microbial route. High cell density fermentation techniques overcome these limitations. Here, propionic acid (PA) production from glucose and a crude glycerol/glucose mixture was evaluated using Acidipropionibacterium acidipropionici
-
Potential use of proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibition and prevention method in viral infection Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-25 Khursheed Muzammil, Mohammad Hosseini Hooshiar, Shirin Varmazyar, Thabit Moath Omar, Manal Morad Karim, Sadeq Aadi, Shaylan Kalavi, Saman Yasamineh
Cellular lipid membranes serve as the primary barrier preventing viral infection of the host cell and provide viruses with a critical initial point of contact. Occasionally, viruses can utilize lipids as viral receptors. Viruses depend significantly on lipid rafts for infection at virtually every stage of their life cycle. The pivotal role that proprotein convertase subtilisin/kexin Type 9 (PCSK9)
-
LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-25 He Liu, Xuemei Wei, Zhefen Wang, Xiaonan Huang, Mengyang Li, Zhen Hu, Kexin Zhang, Qiwen Hu, Huagang Peng, Weilong Shang, Yi Yang, Yuting Wang, Shuguang Lu, Xiancai Rao
Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active phage particles or phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient phage therapeutic regimens have yet to be established. In this study, we used an enrichment procedure to isolate phages
-
Temporal dynamics of stress response in Halomonas elongata to NaCl shock: physiological, metabolomic, and transcriptomic insights Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-23 Junxiong Yu, Yue Zhang, Hao Liu, Yuxuan Liu, Ali Mohsin, Zebo Liu, Yanning Zheng, Jianmin Xing, Jing Han, Yingping Zhuang, Meijin Guo, Zejian Wang
The halophilic bacterium Halomonas elongata is an industrially important strain for ectoine production, with high value and intense research focus. While existing studies primarily delve into the adaptive mechanisms of this bacterium under fixed salt concentrations, there is a notable dearth of attention regarding its response to fluctuating saline environments. Consequently, the stress response of
-
Mutagenetic analysis of the biosynthetic pathway of tetramate bripiodionen bearing 3-(2H-pyran-2-ylidene)pyrrolidine-2,4-dione skeleton Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-21 Haixia Zang, Yijia Cheng, Mengjia Li, Lin Zhou, Li-Li Hong, Hai Deng, Hou-Wen Lin, Yongjun Zhou
Natural tetramates are a family of hybrid polyketides bearing tetramic acid (pyrrolidine-2,4-dione) moiety exhibiting a broad range of bioactivities. Biosynthesis of tetramates in microorganisms is normally directed by hybrid polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) machineries, which form the tetramic acid ring by recruiting trans- or cis-acting thioesterase-like Dieckmann
-
Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-20 Christoph Köppl, Wolfgang Buchinger, Gerald Striedner, Monika Cserjan-Puschmann
Escherichia coli is one of the most commonly used host organisms for the production of biopharmaceuticals, as it allows for cost-efficient and fast recombinant protein expression. However, challenging proteins are often produced with low titres or as inclusion bodies, and the manufacturing process needs to be developed individually for each protein. Recently, we developed the CASPONTM technology, a
-
Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-16 Jonas L. Ravn, João H.C. Manfrão-Netto, Jana B. Schaubeder, Luca Torello Pianale, Stefan Spirk, Iván F. Ciklic, Cecilia Geijer
The abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial conversion into valuable compounds. By engineering strains of the widely employed cell factory Saccharomyces cerevisiae with the ability to directly hydrolyze and ferment GX polymers, we can avoid the need for harsh chemical pretreatments and costly enzymatic hydrolysis
-
Production, characterization and biomedical potential of biosurfactants produced by haloalkaliphilic archaea from Wadi El-Natrun, Egypt Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-14 Basma T. Alghamrawy, Ghada E. Hegazy, Soraya A. Sabry, Hanan Ghozlan
Extreme halophilic archaea that can live in high saline environments can offer potential applications in different biotechnological fields. This study delves into the fascinating field of halophilic archaea and their ability to produce biosurfactants. Some strains of haloarchaea were isolated from Wadi El-Natrun and were screened for biosurfactants production in a standard basal medium using emulsification
-
RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoring Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-14 Muhammad Faisal, Aditya P. Sarnaik, Nandini Kannoju, Nima Hajinajaf, Muhammad Javaid Asad, Ryan W. Davis, Arul M. Varman
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been conducted for improving its activity in photosynthesis through approaches like protein engineering. Concurrently, multiple biochemical and radiolabeling assays have been developed for determining its activity. Although these existing assays yield reliable results, they
-
The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-14 Rafał Jastrząb, Rafał Tomecki, Aneta Jurkiewicz, Damian Graczyk, Agnieszka K. Szczepankowska, Jennifer Mytych, Damian Wolman, Pawel Siedlecki
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among the various therapeutic approaches, interventions involving lactic acid bacteria (LAB) as probiotics and postbiotics have emerged as promising candidates for treating and preventing CRC. While
-
Development of hybrid biomicroparticles: cellulose exposing functionalized fusion proteins Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-14 Joanna Żebrowska, Piotr Mucha, Maciej Prusinowski, Daria Krefft, Agnieszka Żylicz-Stachula, Milena Deptuła, Aneta Skoniecka, Agata Tymińska, Małgorzata Zawrzykraj, Jacek Zieliński, Michał Pikuła, Piotr M. Skowron
One of the leading current trends in technology is the miniaturization of devices to the microscale and nanoscale. The highly advanced approaches are based on biological systems, subjected to bioengineering using chemical, enzymatic and recombinant methods. Here we have utilised the biological affinity towards cellulose of the cellulose binding domain (CBD) fused with recombinant proteins. Here we
-
Improving furfural tolerance in a xylose-fermenting yeast Spathaspora passalidarum CMUWF1–2 via adaptive laboratory evolution Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-13 Thanyalak Saengphing, Pachara Sattayawat, Thitisuda Kalawil, Nakarin Suwannarach, Jaturong Kumla, Mamoru Yamada, Watanalai Panbangred, Nadchanok Rodrussamee
Spathaspora passalidarum is a yeast with the highly effective capability of fermenting several monosaccharides in lignocellulosic hydrolysates, especially xylose. However, this yeast was shown to be sensitive to furfural released during pretreatment and hydrolysis processes of lignocellulose biomass. We aimed to improve furfural tolerance in a previously isolated S. passalidarum CMUWF1−2, which presented
-
Unveiling the silver lining: examining the effects of biogenic silver nanoparticles on the growth dynamics of in vitro olive shoots Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-13 Mohamed S. Hasanin, Sayed A.M. Hassan, A. M. AbdAllatif, Osama M. Darwesh
The current study aimed to evaluate the effects of biogenic silver nanoparticles (AgNPs) on growth behavior and leaf anatomy of in vitro growing shoots of ‘Picual’ and ‘Dolce’ olive cultivars. Biosynthesis of AgNPs was carried out using the cell-free filtrate of Fusarium oxysporum. The dimension and shape of the synthesized AgNPs have been analyzed using spectroscopy and topography analysis tools,
-
Production and bioprocessing of Taxol from Aspergillus niger, an endophyte of Encephalartos whitelockii, with a plausible biosynthetic stability: antiproliferative activity and cell cycle analysis Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-12 Asmaa Gamal, Eman Fikry, Nora Tawfeek, Azza M. El-Shafae, Ashraf S. A. El-Sayed, Maher M. El-Domiaty
The biosynthetic potency of Taxol by fungi raises their prospective to be a platform for commercial production of Taxol, nevertheless, the attenuation of its productivity with the fungal storage, is the challenge. Thus, screening for a novel fungal isolate inhabiting ethnopharmacological plants, with a plausible metabolic stability for Taxol production could be one of the most affordable approaches
-
Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-12 DA. Rzechonek, M. Szczepańczyk, I. Borodina, C. Neuvéglise, AM. Mirończuk
Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether
-
Effective synthesis of high-content fructooligosaccharides in engineered Aspergillus niger Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-09 Xiufen Wan, Lu Wang, Jingjing Chang, Jing Zhang, Zhiyun Zhang, Kewen Li, Guilian Sun, Caixia Liu, Yaohua Zhong
Aspergillus niger ATCC 20611 is an industrially important fructooligosaccharides (FOS) producer since it produces the β-fructofuranosidase with superior transglycosylation activity, which is responsible for the conversion of sucrose to FOS accompanied by the by-product (glucose) generation. This study aims to consume glucose to enhance the content of FOS by heterologously expressing glucose oxidase
-
Correction: Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9 Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-06 Nathaniel Ninyio, Katharina Schmitt, Gladys Sergon, Charlotta Nilsson, Sören Andersson, Nikolai Scherbak
Microbial Cell Factories (2024) 23:39 https://doi.org/10.1186/s12934-023-02290-0 In this article the author name Katharina Schmitt was incorrectly written as Katherina Schmitt. The original article has been corrected. Authors and Affiliations School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden Nathaniel Ninyio & Sören Andersson School of Science and Technology
-
Automated workflow for characterization of bacteriocin production in natural producers Lactococcus lactis and Latilactobacillus sakei Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-03 Valentin Steier, Lisa Prigolovkin, Alexander Reiter, Tobias Neddermann, Wolfgang Wiechert, Sebastian J. Reich, Christian U. Riedel, Marco Oldiges
Lactic acid bacteria are commonly used as protective starter cultures in food products. Among their beneficial effects is the production of ribosomally synthesized peptides termed bacteriocins that kill or inhibit food-spoiling bacteria and pathogens, e.g., members of the Listeria species. As new bacteriocins and producer strains are being discovered rapidly, modern automated methods for strain evaluation
-
Enhancement of cellulolytic enzyme production from intrageneric protoplast fusion of Aspergillus species and evaluating the hydrolysate scavenging activity Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-02 Doaa A. Goda, Huda M. Shakam, Mai E. Metwally, Hager A. Abdelrasoul, Mohamed M. Yacout
Lignocellulosic biomass provides a great starting point for the production of energy, chemicals, and fuels. The major component of lignocellulosic biomass is cellulose, the employment of highly effective enzymatic cocktails, which can be produced by a variety of microorganisms including species of the genus Aspergillus, is necessary for its utilization in a more productive manner. In this regard, molecular
-
Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology Microb. Cell Fact. (IF 6.4) Pub Date : 2024-03-01 Emily J. Kay, Manoj K. Dooda, Joseph C. Bryant, Amanda J. Reid, Brendan W. Wren, Jerry M. Troutman, Matthew A. Jorgenson
Bacterial surface glycans are assembled by glycosyltransferases (GTs) that transfer sugar monomers to long-chained lipid carriers. Most bacteria employ the 55-carbon chain undecaprenyl phosphate (Und-P) to scaffold glycan assembly. The amount of Und-P available for glycan synthesis is thought to be limited by the rate of Und-P synthesis and by competition for Und-P between phosphoglycosyl transferases
-
Shared and more specific genetic determinants and pathways underlying yeast tolerance to acetic, butyric, and octanoic acids Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-29 Marta N. Mota, Madalena Matos, Nada Bahri, Isabel Sá-Correia
The improvement of yeast tolerance to acetic, butyric, and octanoic acids is an important step for the implementation of economically and technologically sustainable bioprocesses for the bioconversion of renewable biomass resources and wastes. To guide genome engineering of promising yeast cell factories toward highly robust superior strains, it is instrumental to identify molecular targets and understand
-
Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-28 Théo Chades, Raphaël Le Fèvre, Imène Chebbi, Karine Blondeau, François Guyot, Edouard Alphandéry
We report the successful fabrication of a pharmaceutical cellular bank (PCB) containing magnetotactic bacteria (MTB), which belong to the Magnetospirillum gryphiswaldense MSR1 species. To produce such PCB, we amplified MTB in a minimal growth medium essentially devoid of other heavy metals than iron and of CMR (Carcinogenic, mutagenic and reprotoxic) products. The PCB enabled to acclimate MTB to such
-
Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-28 Ana García-Franco, Patricia Godoy, Estrella Duque, Juan L. Ramos
We are interested in converting second generation feedstocks into styrene, a valuable chemical compound, using the solvent-tolerant Pseudomonas putida DOT-T1E as a chassis. Styrene biosynthesis takes place from L-phenylalanine in two steps: firstly, L-phenylalanine is converted into trans-cinnamic acid (tCA) by PAL enzymes and secondly, a decarboxylase yields styrene. This study focuses on designing
-
Production and characterization of melanin pigment from black fungus Curvularia soli AS21 ON076460 assisted gamma rays for promising medical uses Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-26 Amira S. Abd-EL-Aziz, Nermine N. Abed, Amira Y. Mahfouz, Rasha Mohammad Fathy
Owing to the growing need for natural materials in different fields, studying melanin production from biological sources is imperative. In the current study, the extracellular melanin pigment was produced by the fungus Curvularia soli AS21 ON076460. The factors that affect the production of melanin were optimized by the Plackett-Burman design (P-BD). The effect of gamma irradiation on melanin productivity
-
“High-throughput screening of catalytically active inclusion bodies using laboratory automation and Bayesian optimization” Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Laura Marie Helleckes, Kira Küsters, Christian Wagner, Rebecca Hamel, Ronja Saborowski, Jan Marienhagen, Wolfgang Wiechert, Marco Oldiges
In recent years, the production of inclusion bodies that retain substantial catalytic activity was demonstrated. These catalytically active inclusion bodies (CatIBs) are formed by genetic fusion of an aggregation-inducing tag to a gene of interest via short linker polypeptides. The resulting CatIBs are known for their easy and cost-efficient production, recyclability as well as their improved stability
-
Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Neetu Rajak, Trishna Dey, Yash Sharma, Vedanth Bellad, Pundi N. Rangarajan
Komagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase (PEPCK) is induced by glutamate. Glutamate-inducible synthesis of these enzymes is regulated by Rtg1p, a cytosolic, basic helix-loop-helix protein. Here, we report food-grade monosodium glutamate (MSG)-inducible recombinant protein
-
Multienzymatic biotransformation of flavokawain B by entomopathogenic filamentous fungi: structural modifications and pharmacological predictions Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Paweł Chlipała, Tomasz Tronina, Monika Dymarska, Monika Urbaniak, Ewa Kozłowska, Łukasz Stępień, Edyta Kostrzewa-Susłow, Tomasz Janeczko
Flavokawain B is one of the naturally occurring chalcones in the kava plant (Piper methysticum). It exhibits anticancer, anti-inflammatory and antimalarial properties. Due to its therapeutic potential, flavokawain B holds promise for the treatment of many diseases. However, due to its poor bioavailability and low aqueous solubility, its application remains limited. The attachment of a sugar unit impacts
-
Lead remediation by geological fluorapatite combined with Penicillium Oxalicum and Red yeast Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Qiang Guan, Xiaohui Cheng, Yue He, Yifan Yan, Lei Zhang, Zhan Wang, Liangliang Zhang, Da Tian
Phosphate solubilizing fungi Penicillium oxalicum (POX) and Red yeast Rhodotorula mucilaginosa (Rho) have been applied in Pb remediation with the combination of fluorapatite (FAp), respectively. The secretion of oxalic acid by POX and the production of extracellular polymers (EPS) by Rho dominate the Pb remediation. In this study, the potential of Pb remediation by the fungal combined system (POX and
-
Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Valériane Malika Keita, Yi Qing Lee, Meiyappan Lakshmanan, Dave Siak-Wei Ow, Paul Staniland, Jessica Staniland, Ian Savill, Kang Lan Tee, Tuck Seng Wong, Dong-Yup Lee
Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared
-
A genome-reduced Corynebacterium glutamicum derivative discloses a hidden pathway relevant for 1,2-propanediol production Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Daniel Siebert, Erich Glawischnig, Marie-Theres Wirth, Mieke Vannahme, Álvaro Salazar-Quirós, Annette Weiske, Ezgi Saydam, Dominik Möggenried, Volker F. Wendisch, Bastian Blombach
1,2-propanediol (1,2-PDO) is widely used in the cosmetic, food, and drug industries with a worldwide consumption of over 1.5 million metric tons per year. Although efforts have been made to engineer microbial hosts such as Corynebacterium glutamicum to produce 1,2-PDO from renewable resources, the performance of such strains is still improvable to be competitive with existing petrochemical production
-
Recombinant L. lactis vaccine LL-plSAM-WAE targeting four virulence factors provides mucosal immunity against H. pylori infection Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-24 Furui Zhang, Linhan Ni, Zhen Zhang, Xuegang Luo, Xuequan Wang, Wenmiao Zhou, Jiale Chen, Jing Liu, Yuliang Qu, Kunmei Liu, Le Guo
Helicobacter pylori (H. pylori) causes chronic gastric disease. An efficient oral vaccine would be mucosa-targeted and offer defense against colonization of invasive infection in the digestive system. Proteolytic enzymes and acidic environment in the gastrointestinal tract (GT) can, however, reduce the effectiveness of oral vaccinations. For the creation of an edible vaccine, L. lactis has been proposed
-
Production and characterization of novel marine black yeast’s exopolysaccharide with potential antiradical and anticancer prospects Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-22 Eman H. Zaghloul, Hala H. Abdel-Latif, Asmaa Elsayis, Sahar W.M. Hassan
The marine black yeasts are characterized by the production of many novel protective substances. These compounds increase their physiological adaptation to multi-extreme environmental stress. Hence, the exopolysaccharide (EPS) producing marine black yeast SAHE was isolated in this study. It was molecularly identified as Hortaea werneckii (identity 98.5%) through ITS1 and ITS4 gene sequencing analysis
-
Physicochemical characterization of polyhydroxybutyrate (PHB) produced by the rare halophile Brachybacterium paraconglomeratum MTCC 13074 Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-22 Teja Mandragutti, Tura Safawo Jarso, Sudhakar Godi, S Sharmila Begum, Beulah K
Polyhydroxybutyrate is a biopolymer produced by bacteria and archaea under nitrogen-limiting conditions. PHB is an essential polymer in the bioplastic sector because of its biodegradability, eco-friendliness, and adaptability. The characterization of PHB is a multifaceted process for studying the structure and its properties. This entire aspect can assure the long-term viability and performance attributes
-
Analysis of heterologous expression of phaCBA promotes the acetoin stress response mechanism in Bacillus subtilis using transcriptomics and metabolomics approaches Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-21 Tao Li, Haixiang Li, Lei Zhong, Yufei Qin, Gege Guo, Zhaoxing Liu, Ning Hao, Pingkai Ouyang
Acetoin, a versatile platform chemical and popular food additive, poses a challenge to the biosafety strain Bacillus subtilis when produced in high concentrations due to its intrinsic toxicity. Incorporating the PHB synthesis pathway into Bacillus subtilis 168 has been shown to significantly enhance the strain’s acetoin tolerance. This study aims to elucidate the molecular mechanisms underlying the
-
Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-18 Kateryna Kukil, Pia Lindberg
Phenylpropanoids are a large group of plant secondary metabolites with various biological functions, derived from aromatic amino acids. Cyanobacteria are promising host organisms for sustainable production of plant phenylpropanoids. We have previously engineered Synechocystis sp. PCC 6803 to produce trans-cinnamic acid (tCA) and p-coumaric acid (pCou), the first intermediates of phenylpropanoid pathway
-
Genetic characterization of a novel Salinicola salarius isolate applied for the bioconversion of agro-industrial wastes into polyhydroxybutyrate Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-17 Shymaa A. Abdelrahman, Olfat S. Barakat, Marwa N. Ahmed
Polyhydroxybutyrate (PHB) has emerged as a promising eco-friendly alternative to traditional petrochemical-based plastics. In the present study, we isolated and characterized a new strain of Salinicola salarius, a halophilic bacterium, from the New Suez Canal in Egypt and characterized exclusively as a potential PHB producer. Further genome analysis of the isolated strain, ES021, was conducted to identify
-
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-17 Bingjie Cheng, Keyang Yu, Xing Weng, Zhaojun Liu, Xuewu Huang, Yuhong Jiang, Shuai Zhang, Shuyan Wu, Xiaoyuan Wang, Xiaoqing Hu
Pichia pastoris is a widely utilized host for heterologous protein expression and biotransformation. Despite the numerous strategies developed to optimize the chassis host GS115, the potential impact of changes in cell wall polysaccharides on the fitness and performance of P. pastoris remains largely unexplored. This study aims to investigate how alterations in cell wall polysaccharides affect the
-
Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida Microb. Cell Fact. (IF 6.4) Pub Date : 2024-02-16 Yannic S. Ackermann, Jan de Witt, Mariela P. Mezzina, Christoph Schroth, Tino Polen, Pablo I. Nikel, Benedikt Wynands, Nick Wierckx
Bio-upcycling of plastics is an emerging alternative process that focuses on extracting value from a wide range of plastic waste streams. Such streams are typically too contaminated to be effectively processed using traditional recycling technologies. Medium-chain-length (mcl) diols and dicarboxylates (DCA) are major products of chemically or enzymatically depolymerized plastics, such as polyesters