单分子检测
(SINGLE MOLECULE DETECTION)
单分子检测术技能够在纳米空间内捕获单个分子,从而实现超灵敏检测。与大多数仅监测一个参数(通常是信号强度)的传统检测方法不同,纳米孔传感器可以从单次测量中同时获取多重信息(单个分子的信号强度、持续时间、信号频率等)。由于信息维度的增加,纳米孔技术的分辨率高,可以从混合物中检测出某一种目标分析物,甚至实现多种分析物的同时检测。此外,纳米孔传感器还可以在多种实验条件下工作(如高浓度盐溶液、高黏度溶液、高温、低pH溶液、高pH溶液等),这些特点使得纳米孔单分子检测技术具有广泛的应用前景。
技术原理
(TECHNICAL PRINCIPLE)
通过电场力驱动单个分子(通常为单链核酸、蛋白质、有机小分子、金属离子等)穿过纳米尺寸的蛋白孔道,由于不同分子通过纳米孔道时会产生不同阻断幅度和阻断持续时间的电流信号,可根据电流信号的指纹信息识别每个分子的特征信息,实现对单个分子的精准、快速检测。
纳米孔单分子检测原理图
西安思莫徳科技有限公司是一家生产单分子检测仪的高科技企业,落地秦创原。公司核心是生产和销售单分子检测仪等各类科学仪器,面向高校、研究所、食品检测、生命科学领域,提供单分子检测仪器和检测服务。
产品优势
(PRODUCT ADVANTAGES)
西安思莫徳科技有限公司生产的纳米孔单分子检测仪器(SIMOLDE NanoF1)是国内首台成熟的单分子专用检测设备,具有集成便携、高性能、可定制、噪音低等核心优势。自主研发了独有的专用集成电路,实现了全信号链的芯片设计与集成系统。
SIMOLDE SMD F1 Basic型号产品外观图
集成便携:检测设备体积小(SIMOLDE SMD F1 Basic型号产品尺寸约26cm*15cm*9cm)、抗震和电磁屏蔽性能好,实现现场便携检测。
信号响应速度快:最高带宽100 kHz以上,采样速度最高250 kHz。
可定制:根据客户需求,以下参数均可定制:量程范围:±2.5 nA至±2.5 μA;滤波截止频率:256 Hz至100 kHz;带宽范围:1 kHz至100 kHz;施加电压范围:±200 mV至±2 V。
噪音低:采用标准模块(图b)进行实测,在同样带宽和采样速率下,与elements公司的商业化eONE HS型号仪器对比(图a右),本公司产品(SIMOLDE SMD F1 Basic型号)的噪音更低(图a左)。
近三年基于纳米孔检测发表的部分高水平论文:
Highly shape-and size-tunable membrane nanopores made with DNA[J]. Nature Nanotechnology, 2022: 1-6.
Multiple rereads of single proteins at single–amino acid resolution using nanopores[J]. Science, 2021, 374, 1509-1513.
Handling a protein with a nanopore machine[J]. Nature Chemistry, 2021, 13(12): 1160-1162.
Directional conformer exchange in dihydrofolate reductase revealed by single-molecule nanopore recordings[J]. Nature Chemistry, 2020, 12(5): 481-488.
Bottom-up fabrication of a proteasome–nanopore that unravels and processes single proteins[J]. Nature chemistry, 2021, 13(12): 1192-1199.
De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide[J]. Nature nanotechnology, 2022, 17(1): 67-75.
Nanopore electro-osmotic trap for the label-free study of single proteins and their conformations[J]. Nature Nanotechnology, 2021, 16(11): 1244-1250.
Mapping the conformational energy landscape of Abl kinase using ClyA nanopore tweezers[J]. Biophysical Journal, 2022, 121(3): 339a.
On the origins of conductive pulse sensing inside a nanopore[J]. Nature Communications, 2022, 13(1): 1-11.
Disentangling the recognition complexity of a protein hub using a nanopore[J]. Nature communications, 2022, 13(1): 1-11.
Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection[J]. Nature communications, 2021, 12(1): 1-8.
Nanopore-based protein identification[J]. Journal of the American Chemical Society, 2022, 144(6): 2716-2725.
A nanopore sensing assay resolves cascade reactions in a multienzyme system[J]. Angewandte Chemie International Edition, 2022, 61(20): e202200866.
Machine learning assisted simultaneous structural profiling of differently charged proteins in a Mycobacterium smegmatis porin A (MspA) electroosmotic trap[J]. Journal of the American Chemical Society, 2022, 144(2): 757-768.
Non-binary encoded nucleic acid barcodes directly readable by a nanopore[J]. Angewandte Chemie International Edition, 2022, 61(20): e202116482.
Programmable nano-reactors for stochastic sensing[J]. Nature communications, 2021, 12(1): 1-13.
Aerolysin nanopores decode digital information stored in tailored macromolecular analytes[J]. Science Advances, 2020, 6(50): eabc2661.
Biological nanopore approach for single‐molecule protein sequencing[J]. Angewandte Chemie, 2021, 133(27): 14862-14873.
Giant single molecule chemistry events observed from a tetrachloroaurate (III) embedded Mycobacterium smegmatis porin A nanopore[J]. Nature communications, 2019, 10(1): 1-11.
Protein identification by nanopore peptide profiling[J]. Nature communications, 2021, 12(1): 1-9.
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