“Multiplex immunochromatographic platform based on crystal violet tag for simultaneous detection of streptomycin and chloramphenicol” published in Food Chemistry. The first author is Shaochi Wang, and the communication author is Professor Jianlong Wang.

Antibiotic abuse has caused serious health risks for human beings for long. Here, an effective multiplex immunochromatographic platform (MICP) with synthesis-free and cost-effective merits is established for simultaneous detection of streptomycin (STR) and chloramphenicol (CAP) on a single immunochromatographic assay (ICA) strip. Adopting crystal violet (CV) as a signal tag for multiplex ICA allows for direct coupling with multiple antibodies in several minutes. By combining CV and ICA perfectly, this convenient strategy offers improvements in convenience, speed, flexibility, and portability. The proposed method has been validated in milk and honey samples with good applicability and accuracy. It provides rich opportunities for fast and high-throughput on-site detection in the future.

Links:https://doi.org/10.1016/j.foodchem.2022.133351