Publications at SJTU
23. Zhiheng Deng#, Shixian Tao#, Yunxiang Du#, Yulei Li#, Liying Zhang, Qiang Shi, Xiaoru Du, Maoshen, Sun, Zebin Tong, Man Pan*, Lei Liu*, Huasong Ai*(最后通讯). Allosteric Activation of RNF20/RNF40–RAD6A-Mediated H2BK120 Monoubiquitylation by H2BS112 GlcNAcylation. Nature Chemical Biology, 2025, accepted in princilple.
22. Tianyi Zhang#, Jiqing Zheng#, Zebing Tong#, Zhiheng Deng#, Zaozhen He, Xiangwei Wu, Miao Wang, Yunxiang Du, Ziyu Xu, Shixian Tao, Xiaolin Tian, Haiteng Deng, Man Pan*, Huasong Ai*(共通讯), Lei Liu*. Unique Gluing Effect of ASXL1 K351 Monoubiquitination Stimulates the PR-DUB Activity. Nature Chemical Biology, 2025, accepted in princilple. bioRxiv, 2025, https://doi.org/10.1101/2025.04.25.650627.
21. Zebin Tong#, Xiangwei Wu#, Hongyi Cai#, Shidian Wu, Tianyi Zhang, Zhiheng Deng, Ziyu Xu, Rujing Yuan, Huasong Ai* (共通讯), Lei Liu*, Man Pan*. Structural basis for the ubiquitin E4 enzyme Ufd2-catalyzed K48/K29 branched ubiquitin chains. Nature Chemical Biology, 2025, 10.1038/s41589-025-01985-2.
20. Chuntong Li, Fangyu Zhao, Chong Zuo, Liying Zhang, Yangwode Jing, Xu Li, Guo-Chao Chu, Luyu Shi, Yingyue Zhang, Han Wang, Shuzhe Sun, Maoshen Sun, Huasong Ai*(共通讯),* Lu-Jun Liang,* Jinghong Li*. Design and Semi-synthesis of Ubiquitin Extension Probes for Structural Analysis of Cullin1 Mediated Substrate Polyubiquitination. Journal of the American Chemical Society, 2025, 147, 23878–23890.
19. Zhiheng Deng#, Huasong Ai#,* (共一/共通讯), Qiang Shi#, Jiawei Liang, Zaozhen He, Jiqing Zheng, Haoxiang Li, Liying Zhang, Wei He, Shixian Tao, Qingyun Zheng, Wei He, Man Pan*, Lei Liu*. A Cryptic Interfacial Pocket Uncovered in Full CRL4CRBN–IKZF3 Ubiquitylation Complex Enhances IMiD Efficacy. bioRxiv, 2025, https://doi.org/10.1101/2025.06.08.658527.
18. Yunxiang Du#, Maoshen Sun#, ZhengQing Li#, Xiangwei Wu, Qian Qu*, Huasong Ai* (共通讯), Lei Liu*. Mechanistic Insights into the Stimulation of the Histone H3K9 Methyltransferase Clr4 by Proximal H3K14 Ubiquitination. Science Advances, 2025, 11, eadu1864.
17. Xiangwei Wu#, Huasong Ai# (共一), Junxiong Mao#, Hongyi Cai, Lujun Liang, Zebin Tong, Zhiheng Deng, Qingyun Zheng*, Lei Liu*, Man Pan*. Structural Visualization of HECT-E3 Ufd4 accepting and transferring Ubiquitin to Form K29/K48-branched Polyubiquitination. Nature Communications, 2025, 16, 4313.
16. Shixian Tao#, Wei He#, Chuntong Li, Jiawei Liang, Zebin Tong, Zhiheng Deng, Li-ying Zhang, Huasong Ai*(共通讯), Lu-Jun Liang* & Lei Liu*. Synthesis of an E2-Ub-Nucleosome Conjugate to Capture the E3 Ligase PHF7-Catalyzed H3K14 Ubiquitination Intermediate. Science China Chemistry, 2025, 68, 5871.
15. Qi Shu, Yun Liu, Huasong Ai* (通讯). The Emerging Role of the Histone H2AK13/15 Ubiquitination: Mechanisms of Writing, Reading, and Erasing in DNA Damage Repair and Disease. Cells, 2025, 14(4), 307.
14. Qiang Shi#, Zhiheng Deng#, Liying Zhang#, Zebin Tong, Jia-Bin Li, Guo-Chao Chu*, Huasong Ai* (共通讯), and Lei Liu*. Promotion of RNF168-Mediated Nucleosomal H2A Ubiquitylation by Structurally-defined K63-Polyubiquitylated Linker Histone H1. Angewandte Chemie International Edition, 2025, 64(1):e202413651.
13. Huasong Ai#, Zebin Tong#, Zhiheng Deng#, Qiang Shi, Shixian Tao, Gaoge Sun, Jiawei Liang, Maoshen Sun, Xiangwei Wu, Qingyun Zheng, Lujun Liang, Hang Yin, Jia-Bin Li, Shuai Gao, Changlin Tian*, Lei Liu*, Man Pan*. Mechanism of nucleosomal H2A K13/15 monoubiquitination and adjacent dual monoubiquitination by RNF168. Nature Chemical Biology, 2025, 21, 668-680.
Publications prior to SJTU
12. Huasong Ai#, Zaozhen He#, Zhiheng Deng#, Guo-Chao Chu, Qiang Shi, Zebin Tong, Jia-Bin Li, Man Pan*, Lei Liu*. Structural and mechanistic basis for nucleosomal H2AK119 deubiquitination by single-subunit deubiquitinase USP16. Nature Structural & Molecular Biology, 2024, 31, 1745-1755.
11. Huasong Ai#, Maoshen Sun#, Aijun Liu#, Zixian Sun#, Tingting Liu#, Lin Cao, Lujun Liang, Qian Qu, Zichen Li, Zhiheng Deng, Zebin Tong, Guochao Chu, Xiaolin Tian, Haiteng Deng, Suwen Zhao, Jiabin Li*, Zhiyong Lou*, Lei Liu*. H2B Lys34 ubiquitination induces nucleosome distortion to stimulate Dot1L activity. Nature Chemical Biology, 2022, 18, 972-980.
10. Huasong Ai#, Guochoa Chu#, Qingyue Gong#, Zhiheng Deng, Zebin Tong, Xin Liu, Fan Yan, Jiabin Li*, Changlin Titan*, Lei Liu*. Chemical Synthesis of Post-Translationally Modified H2AX Reveals Redundancy in Interplay between Histone Phosphorylation, Ubiquitination, and Methylation on the Binding of 53BP1 with Nucleosomes. Journal of the American Chemical Society, 2022, 144, 18329-18337.
9. Huasong Ai#, Zebin Tong#, Zhiheng Deng, Jiakun Tian, Liying Zhang, Maoshen Sun, Yunxiang Du, Ziyu Xu, Qiang Shi, Lujun Liang, Qingyun Zheng, Jiabin Li, Man Pan*, Lei Liu*. Synthetic E2-Ub-nucleosome conjugates for studying nucleosome ubiquitination. Chem, 2023, 9, 1221-1240.
8. Huasong Ai, Lei Liu*. Paralog-specific recognition. Nature Chemical Biology, 2023, 5, 542-543.
7. Huasong Ai, Man Pan, Lei Liu*. Chemical Synthesis of Human Proteoforms and Application in Biomedicine. ACS Central Science, 2024, 10, 1442-1459.
6. Huasong Ai, Yu Guo, Demeng Sun, Sanling Liu, Yunkun Qi, Jing Guo, Qian Qu, Qingyue Gong, Suwen Zhao*, Jiabin Li*, Lei Liu*. Examination of the Deubiquitylation Site Selectivity of USP51 by Using Chemically Synthesized Ubiquitylated Histones. ChemBioChem, 2019, 20, 221-229.
5. Huasong Ai, Shuai Peng, Jia-Bin Li*. Chemical methods for studying the crosstalk between histone H2B ubiquitylation and H3 methylation. Journal of Peptide Science, 2021, e3381.
4. Zebin Tong#, Huasong Ai#, *(共一/共通讯), Ziyu Xu#, Kezhang He#, Guo-Chao Chu, Qiang Shi, Zhiheng Deng, Qiaomei Xue, Maoshen Sun, Yunxiang Du, Lujun Liang, Jia-Bin Li, Man Pan*, Lei Liu*. Synovial Sarcoma X Breakpoint 1 Protein uses a cryptic groove to selectively recognize H2AK119Ub Nucleosomes. Nature Structural & Molecular Biology, 2024, 31, 300–310.
3. Zhiheng Deng#, Huasong Ai# (共一), Maoshen Sun#, Zebin Tong#, Yunxiang Du#, Qian Qu, Liying Zhang, Ziyu Xu, Shixian Tao, Qiang Shi, Jia-Bin Li, Man Pan, Lei Liu*. Mechanistic insights into nucleosomal H2B monoubiquitylation mediated by yeast Bre1-Rad6 and its human homolog RNF20/RNF40-hRAD6A. Molecular Cell, 2023, 83, 3080-3094.E14.
2. Zichen Li#, Zebin Tong#, Qingyue Gong#, Huasong Ai* (共通讯), Shuai Peng, Cong Chen, Guo-Chao Chu, Jia-Bin Li*. The expedient, CAET-assisted synthesis of dual-monoubiquitinated histone H3 enables evaluation of its interaction with DNMT1. Chemical Science, 2023,14, 5681-5688.
1. Yunkun Qi#, Huasong Ai# (共一), Yiming Li, Binghui Yan. Total Chemical Synthesis of Modified Histones. Frontiers in Chemistry, 2018, 6, 19.
1. Man Pan#＊, Qingyun Zheng#, Tian Wang#, Lujun Liang#, Chong Zuo, Ruichao Ding, Huasong Ai, Yuan Xie, Si Dong, Yuanyuan Yu＊, Lei Liu＊, Minglei Zhao＊. Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1. Nature, 2021, 600, 334–338.
2. Man Pan#＊, Yuanyuan Yu#, Huasong Ai; Qingyun Zheng, Yuan Xie, Lei Liu＊, Minglei Zhao＊. Mechanistic insight into substrate processing and allosteric inhibition of human p97. Nat. Struct. Mol. Biol., 2021, 28, 614-625.
3. Man Pan#, Qingyun Zheng#, Yuanyuan Yu#, Huasong Ai, Yuan Xie, Xin Zeng, Chu Wang, Lei Liu＊, Minglei Zhao＊. Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative, Nat. Commun., 2021, 12(1): 121.
4. Qiang Shi, Zebin Tong, Zhiheng Deng, Ziyu Xu, Huasong Ai, Yang Liu, Lei Liu*. Chemical mechanisms of nucleosomal histone ubiquitination by RING-type E3 enzymes. Sci. Sin. Chim., 2023, 53.
5. Ziyu Xu, Huasong Ai, Maoshen Sun, Jiakun Tian, Lei Liu*. Advances on structural mechanisms of ubiquitinated nucleosome complexes. Sci. Sin. Chim., 2020, 50, 415-436.
6. Guo-Chao Chu, Man Pan, Jiabin Li, Sanling Liu, Chong Zuo, Ze-Bin Tong, Jing-Si Bai, Qingyue Gong, Huasong Ai, Jian Fan, Xianbin Meng, Yi-Chao Huang, Jing Shi, Haiteng Deng, Changlin Tian*, Yi-Ming Li*, Lei Liu*. Cysteine-aminoethylation-assisted chemical ubiquitination of recombinant histones. J. Am. Chem. Soc., 2019, 141, 3654-3663.
7. Chong Zuo, Ruichao Ding, Xiangwei Wu, Yuanxia Wang, Guo-Chao Chu, Lu-Jun Liang, Huasong Ai, Ze-Bin Tong, Junxiong Mao, Qingyun Zheng, Tian Wang, Zichen Li, Lei Liu*, Demeng Sun*. Thioester-assisted Sortase-A – mediated ligation. Angew. Chem. Int. Ed., 2022, 61, e202201887.
8. Zhiheng Deng, Huasong Ai, Chengpiao Lu, Jia-Bin Li*. The Bre1/Rad6 machinery: writing the central histone ubiquitin mark on H2B and beyond. Chromosome Res. 2020, 28(3-4):247-258.
9. Zebin Tong, Huasong Ai, Jia-Bin Li*. The Mechanism of Chromatin Remodeler SMARCAD1/Fun30 in Response to DNA Damage. Front. Cell Dev. Biol. 2020 8:560098.
10. Jia-Bin Li#, Yun-Kun Qi#, Qiao-Qiao He, Huasong Ai, San-Ling Liu, Jia-Xing Wang, Ji-Sheng Zheng, Lei Liu, Changlin Tian*. Chemically synthesized histone H2A Lys13 di-ubiquitination promotes binding of 53BP1 to nucleosomes. Cell Res. 2018, 28, 257-260.
11. Yunkun Qi, Qiaoqiao He, Huasong Ai, Jing Guo, Jia-Bin Li*. The convergent chemical synthesis of histone H3 protein for site-specific acetylation at Lys56 and ubiquitination at Lys122. Chem. Commun. 2017, 53(29):4148-4151.
12. Chen-Chen Chen#, Shuai Gao#, Huasong Ai, Qian Qu, Chang-Lin Tian& Yi-Ming Li*. Racemic X-ray structure of L-type calcium channel antagonist Calciseptine prepared by total chemical synthesis. Sci. China Chem. 2018, 61, 702-707.
13. Yun-Kun Qi , Qiao-Qiao He , Huasong Ai , Jia-Bin Li* , Ji-Shen Zheng*. Convergent Total Synthesis of Histone H2B Protein with Site-Specific Ubiquitination at Lys120. Synlett, 2017; 28, 1907-1912.
14. Guo-Chao Chu#, Lu-Jun Liang#, Rui Zhao#, Yan-Yan Guo, Chun-Tong Li, Chong Zuo, Huasong Ai, Xiao Hua, Zi-Chen Li, Yi-Ming Li, Lei Liu. Ferricyanide-promoted Oxidative Activation and Ligation of Protein Thioacids in Neutral Aqueous Media. CCS Chem. 2024, 6, 2031–2043.
15. Li-Li Feng#, Shu-Ying Bie#, Zhi-Heng Deng#, Shao-Mei Bai#, Jie Shi, Cao-Litao Qin, Huan-Lei Liu, Jia-Xu Li, Wan-Ying Chen, Jin-Ying Zhou, Chun-Mei Jiao, Yi Ma, Meng-Bo Qiu, Hua-Song Ai, Jian Zheng, Yun-Long Wang*, Mien-Chie Hung*, Xiang-Bo Wan*, Xin-Juan Fan*. Ubiquitin-induced RNF168 condensation promotes DNA double-strand break repair. Proc. Nat. Acad. Sci. 2024, 121, e2322972121.
