近日，2022级博士生裴龙生在SCI一区期刊Chemical Engineering Journal (IF=13.2) 发表FCA-琼脂糖凝胶-GOx-Ag-COF@CNT贴片无创电化学传感器检测间质液中葡萄糖“Detection of glucose in interstitial fluid by FCA-agarose gel-GOx-Ag-COF@CNT patch non-invasive electrochemical sensor”。

原文链接：https://doi.org/10.1016/j.cej.2025.166131

Abstract

Continuous glucose monitoring is favored by diabetic patients owing to its convenience, real-time monitoring, and painlessness as compared with painful and inconvenient finger-stick monitoring. However, it still suffers from discomfort resulting from skin irritation caused by microneedle or current of reverse iontophoresis (RI) and low sensitivity caused by a low extraction rate of RI. Herein, an accurate electrochemical biosensor based on RI was developed to achieve continuous monitoring of blood glucose in interstitial fluid (ISF) in a non-invasive and painless manner. The biosensor is constructed by using ferrocenecarboxylic acid (FCA)-agarose gel-glucose oxidase (GOx)-Ag-covalent organic frameworks (COF)@carbon nanotube (CNT). The COF grew vertically on CNT effectively immobilized lots of GOx and maintained the long-term bioactivity of GOx as well as enriched glucose to improve the sensitivity of biosensor greatly. The CNT and Ag enhanced the electron transfer of the FCA electron mediator greatly. Ag with good antimicrobial abiltiy, combined with biocompatible and elastic agarose gel improved the comfort of biosensor during long-term wearing well. Based on RI technology, the FCA-agarose gel-GOx-Ag-COF@CNT patch biosensor can realize the monitoring of glucose in ISF with a detection limit of 0.020 mM and a sensitivity of 1.049 μA mM^-1. The accurate yet comfortable non-invasive glucose biosensor provides good wearability and is expected to be integrated with smart devices as wearable health monitoring platforms.

Scheme 1. Schematic diagram of the preparation of FCA-agarose gel-GOx-Ag-COF@CNT/SPE sensor.