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Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry

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Abstract

Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS2) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS2 analyses of Des and Isodes are performed using stable-isotope-labeled desmosine d4 (labeled-Des) as an internal standard in different biological fluids, such as urine and serum. The method demonstrated linearity over two orders of magnitude with a detection limit of 0.02 ng/μL in both urine and serum without enrichment prior to mass spectrometry, and relative standard deviation of < 5%. The method is used to evaluate the time-dependent degradation of Des upon UV irradiation (254 nm) and found to be consistent with quantification by 1H NMR. This is the first characterized MALDI-MS2 method for quantification of Des and Isodes and illustrates the potential of MALDI-ion trap MS2 for effective quantification of biomolecules. The reported method represents improvement over current liquid chromatography-based methods with respect to analysis time and solvent consumption, while maintaining similar analytical characteristics.

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Acknowledgements

The authors acknowledge Dr. Sanjai Kumar and Ashif Bhuiyan from Queens College/City University of New York for their assistance in conducting the 1H NMR study. G.B. acknowledges support from the NIH award 2SC1GM086268.

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Authors and Affiliations

  1. Department of Chemistry, York College of The City University of New York, 94-20 Guy R. Brewer Blvd, Jamaica, NY, 11451-0001, USA

    Pratikkumar Rathod, Manjeet Kaur, Hsin-Pin Ho, Marissa E. Louis, Jong I. Lee & Emmanuel J. Chang

  2. Chemistry Doctoral Program, The Graduate Center of The City University of New York, 365 5th Ave, New York, NY, 10016, USA

    Pratikkumar Rathod & Emmanuel J. Chang

  3. Department of Physics, The Graduate Center of The City University of New York, 365 5th Ave, New York, NY, 10016, USA

    Basant Dhital & Gregory S. Boutis

  4. Department of Physics, Brooklyn College of The City University of New York, Brooklyn, NY, 11210, USA

    Philip Durlik & Gregory S. Boutis

  5. Department of Natural Sciences, LaGuardia Community College, The City University of New York, 31-10 Thomson Ave, Long Island City, NY, 11101, USA

    Kevin J. Mark

  6. Biochemistry Doctoral Program, The Graduate Center of The City University of New York, 365 5th Ave, New York, NY, 10016, USA

    Emmanuel J. Chang

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  1. Pratikkumar Rathod
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  9. Jong I. Lee
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  10. Emmanuel J. Chang
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Corresponding author

Correspondence to Emmanuel J. Chang.

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All human specimens were provided with informed consent of the donor. These studies were conducted under the guidance of the Human Research Protection Program of the City University of New York, and have been performed in accordance with ethical standards.

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The authors declare that they have no conflict of interest.

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Rathod, P., Kaur, M., Ho, HP. et al. Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry. Anal Bioanal Chem 410, 6881–6889 (2018). https://doi.org/10.1007/s00216-018-1288-z

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  • DOI: https://doi.org/10.1007/s00216-018-1288-z

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