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Boronate affinity solid-phase extraction of cis-diol compounds by a one-step electrochemically synthesized selective polymer sorbent

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Abstract

A rational-designed conductive sorbent, poly(thiophene-3-boronic acid) electrochemically deposited on a carbon fiber bundle, was applied for boronate affinity extraction. The coated carbon fiber bundle packed into a poly(ether ether ketone) tube was then successfully used for online solid-phase microextraction–high-performance liquid chromatography analysis of cis-diol compounds. Three kinds of catecholamines (namely, adrenaline, noradrenaline, and dopamine) were used as the test analytes. Good extraction efficiency (more than 600-fold), low limits of detection (0.2 ng·mL-1 for adrenaline, 0.1 ng·mL-1 for noradrenaline and dopamine), and wide linear ranges were obtained. The method was demonstrated to be efficient for analysis of catecholamines in spiked plasma samples, with good recoveries in the range of 92.5–95.71%. This work exhibited several significant advantages, including ease of use, high specificity, and high extraction efficiency. It has been demonstrated that such an electrochemically synthesized sorbent has great potential for research involving cis-diol compounds.

Electrochemical modification, packing of fiber bundles, and extraction mechanism. PEEK poly(ether ether ketone)

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Acknowledgements

The authors gratefully thank the National Natural Science Foundation of China (grant nos. 21375101, 81573384, and 91417301) and the Natural Science Foundation of Hubei (no. 2014CFA077) for financial support of this work.

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Authors and Affiliations

  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Science, Wuhan, Hubei, 430071, China

    Xu Ling & Zilin Chen

  2. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 10080, China

    Xu Ling & Zilin Chen

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  1. Xu Ling
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  2. Zilin Chen
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Correspondence to Zilin Chen.

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The authors declare that they have no competing interests. The study received clearance from the Institutional Animal Ethical Committee of the Committee for the Purpose of Control and Supervision of Experiments on Animals, Wuhan University, Wuhan, China.

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Ling, X., Chen, Z. Boronate affinity solid-phase extraction of cis-diol compounds by a one-step electrochemically synthesized selective polymer sorbent. Anal Bioanal Chem 410, 501–508 (2018). https://doi.org/10.1007/s00216-017-0740-9

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  • DOI: https://doi.org/10.1007/s00216-017-0740-9

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