Issue 16, 2024
From the journal:

Organic & Biomolecular Chemistry

Recent advances in Si-rhodamine-based fluorescent probes for live-cell imaging

Hisashi Ohno, ORCID logo a   Eita Sasaki,ab   Sota Yamada ORCID logo a  and  Kenjiro Hanaoka ORCID logo *ab  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, Japan
E-mail: khanaoka@keio.jp

b Faculty of Pharmacy, Keio University, Tokyo 105-8512, Japan

Abstract

Fluorescence imaging is a powerful technique for visualizing biological events in living samples with high temporal and spatial resolution. Fluorescent probes emitting far-red to near infrared (NIR) fluorescence are particularly advantageous for in vivo imaging due to their high tissue permeability and low autofluorescence, as well as their suitability for multicolor imaging. Among the far-red to NIR fluorophores, Si-rhodamine is one of the most practical fluorophores for the development of tailor-made NIR fluorescent probes because of the relative ease of synthesis of various derivatives, the unique intramolecular spirocyclization behavior, and the relatively high water solubility and high photostability of the probes. This review summarizes these features of Si-rhodamines and presents recent advances in the synthesis and applications of far-red to NIR fluorescent probes based on Si-rhodamines, focusing on live-cell imaging applications such as fluorogenic probes, super-resolution imaging and dye–protein hybrid-based indicators.

Graphical abstract: Recent advances in Si-rhodamine-based fluorescent probes for live-cell imaging

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Article information

DOI
https://doi.org/10.1039/D4OB00130C
Article type
Review Article
Submitted
24 Jan 2024
Accepted
26 Feb 2024
First published
06 Mar 2024
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2024,22, 3099-3108

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Recent advances in Si-rhodamine-based fluorescent probes for live-cell imaging

H. Ohno, E. Sasaki, S. Yamada and K. Hanaoka, Org. Biomol. Chem., 2024, 22, 3099 DOI: 10.1039/D4OB00130C

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