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  • Effect of deboning time on the growth of Salmonella, E. coli, aerobic, and lactic acid bacteria during beef sausage processing and storage
    Meat Sci. (IF 3.126) Pub Date : 2018-01-17
    Anuraj T. Sukumaran, Alexander J. Holtcamp, April K. Englishbey, Yan L. Campbell, Taejo Kim, Mark W. Schilling, Thu T.N. Dinh

    The objective of the current study was to determine the effects of deboning time, three steps of sausage processing (grinding, salting, and batter formulation), and storage time (of raw materials and cooked sausage) on the growth (log CFU/g) of aerobic bacteria, lactic acid bacteria, and inoculated Salmonella and E. coli. Beef deboning time did not influence bacterial counts (P ≥ 0.138). However, salting of raw ground beef resulted in a 0.4-log reduction in both aerobic plate count (APC) and Salmonella (P ≤ 0.001). Lactic acid bacteria were increased from non-detectable concentration (0.54 log) on d 0 to 3.8 log on d 120 of vacuum storage (P ≤ 0.019). Salmonella counts were increased (P < 0.001) over storage time (3.2 to 3.3 log CFU/g from d 0 to 10). Results indicated that salting and batter formulation had a greater impact on bacterial counts than rigor state of raw beef.

    更新日期:2018-01-17
  • Interactions between dietary fiber and ferulic acid changed the aggregation of gluten in a whole wheat model system
    LWT - Food Sci. Technol. (IF 2.329) Pub Date : 2018-01-16
    Lianyan Huang, Xiaoshuang Zhang, Huijuan Zhang, Jing Wang

    As the main phenolic acid in wheat bran, ferulic acid (FA) may impact the reactions between disulfide bonds (SS) and sulfhydryl groups (SH) to change gluten proteins aggregation. This study investigated effects of dietary fiber (DF) and FA on secondary structures, thermal properties, and microstructures of gluten proteins. Results showed that the major portion of gluten secondary structures in all test groups was β-sheets (3583–4498 cm2/m2) while α-helices (869–1628 cm2/m2) made up only a small part. The group with 20 g/kg FA and 60 g/kg DF had a similar β-sheet content (4462 cm2/m2) as the control group (4408 cm2/m2), which decreased the damage of gluten structures induced by FA or DF. The total equivalent thiol (SHeq) content, SS content, denaturation temperature (Tp), and enthalpy (△H) of gluten proteins were significantly decreased (P < .05) compared with the control group when DF or FA increased. DF and FA addition also led to a more disordered gluten network shown by scanning electron microscopy (SEM). When DF and FA were added together, the interaction between DF and FA prevented the SS decrease. However, thermal properties analysis of groups including different contents of FA and DF indicated that stability was not improved as expected.

    更新日期:2018-01-17
  • Probiotic properties and stress response of thermotolerant lactic acid bacteria isolated from cooked meat products
    LWT - Food Sci. Technol. (IF 2.329) Pub Date : 2018-01-16
    Annel M. Hernández-Alcántara, Carmen Wacher, M. Goretti Llamas, Paloma López, M. Lourdes Pérez-Chabela

    The aim of this study was to evaluate the probiotic properties of six thermotolerant lactic acid bacteria isolated from cooked meat products. The bacteria were typed, by determination of the DNA sequence of their 16S rRNA coding genes, as one Enterococcus faecium (UAM1 strain) and five Pediococcus pentosaceus (UAM2-UAM6 strains). Under gastric stress conditions the viability of the Pediococci decreased more than five-fold, whereas E. faecium showed a high resistance (61% survival). Exposure to small intestine stress did not drastically affect the survival of any of the strains (less than one-fold decrease), which were able to grow in the presence of 0.3% bile. A hydrophilic surface profile was observed, with higher affinity for chloroform than for xylene. Strains showed high levels of auto-aggregation as well as co-aggregation with Gram-positive and Gram-negative bacterial pathogens. The adherence of E faecium UAM1 to human Caco-2 cells (around 20%) was significantly higher than that obtained with the P. pentosaceus strains (2%–5%) and Lactobacillus acidophilus LA-5 (6%). The overall results indicate that E. faecium UAM1, has probiotic properties that predict its capability to colonize in competition with pathogens in the intestinal tract. This bacterium deserves further investigation for its potential as a component of functional food.

    更新日期:2018-01-17
  • Improving the freeze-thaw stability of soy protein emulsions via combing limited hydrolysis and maillard-induced glycation
    LWT - Food Sci. Technol. (IF 2.329) Pub Date : 2018-01-16
    Jie Yu, Guorong Wang, Xibo Wang, Yeye Xu, Shuang Chen, Xiaodan Wang, Lianzhou Jiang

    The investigation of limited hydrolysis combined with Maillard-induced glycation on improving the freeze-thaw stability of soy protein isolate (SPI) was carried out. Soy protein isolate hydrolysate (SPH) was first prepared by trypsin, with a hydrolysis degree of 2% and 5%. Afterwards, SPI and SPH were conjugated with dextran to form a covalent complex macromolecule, which were named SPI-D, SPH2-D and SPH5-D, respectively. Covalent bond was formed between SPI/SPH and dextran molecules via the glycation reaction has been confirmed by fourier transform infrared (FTIR) spectroscopy analysis. Subsequently, the freeze-thaw stability of SPI-D and SPH-D was evaluated. After three freeze-thaw cycles, the characters of SPH-D emulsions exhibited smaller values than those of SPI-D emulsions in terms of oiling off, particle size, flocculation degree (FD) and coalescence degree (CD). In addition, SPH2-D emulsions were more stable after freeze-thaw treatment compared with SPH5-D emulsions. Optical microscopy analysis also supported the results above.

    更新日期:2018-01-17
  • Boosting Pt oxygen reduction reaction activity and durability by carbon semi-coated titania nanorods for proton exchange membrane fuel cells
    Electrochim. Acta (IF 4.798) Pub Date : 2018-01-17
    P. Dhanasekaran, S. Vinod Selvaganesh, Avanish Shukla, N. Nagaraju, S.D. Bhat

    We report a simple, scalable approach to improve interfacial characteristics of carbon semi-coated titania nanorods-supported-Pt with superior peak power density as compared to Pt/C with thin metal loading of 150 μg cm−2. Thin layer of carbon coated titania nanorod is synthesized by hydrothermal method. Carbon coated titania nanorods boosts the Pt oxygen reduction reaction activity than carbon. The crystal structure, dispersion of platinum nanoparticles, surface morphology and oxidation state are studied by X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy, respectively. Studies using conventional three electrode setup shows that Pt/CCT-30 retains 48% of initial electrochemical surface area even after 40,000 potential cycles between 0.6 and 1.2 V. The solid fuel cell mode accelerated stress durability studies show that thin layer of carbon coated titania nanorods-Pt (Pt/CCT 30) significantly enhances stability and preserves 75% of initial fuel cell performance even after 10,000 potential cycles between 1 and 1.5 V. In comparison, only 20% of performance is retained for Pt supported on carbon after 3000 cycles.

    更新日期:2018-01-17
  • Mesoporous perforated Co3O4 nanoparticles with a thin carbon layer for high performance Li-ion battery anodes
    Electrochim. Acta (IF 4.798) Pub Date : 2018-01-17
    Ji Sun Park, Dong Ok Shin, Churl Seung Lee, Young-Gi Lee, Ju Young Kim, Kwang Man Kim, Kwon Woo Shin

    A facile method for preparing mesoporous perforated Co3O4 nanoparticles with hollow channels and a thin carbon layer was newly designed and achieved with sacrificial carbon nanotube (CNT) templates. The threaded Co3O4 nanoparticles on the CNTs were fabricated through a hybridization process, and a subsequent calcination process produced mesoporous perforated Co3O4 nanoparticles. With a finely tuned calcination, CNTs were sacrificed to generate hollow channels in the Co3O4 nanoparticles and to provide a carbon source for the formation of a few nm-thick carbon layer on the surface of the Co3O4 nanoparticles simultaneously. The prepared mesoporous perforated Co3O4 nanoparticles with a robust electronic conductive layer enabled not only an enhanced electrochemical reactivity from the greatly increased contact area between the electrolyte and electrode but also a high electronic conductivity of the overall electrode so that excellent electrochemical performances (1115.1 mA h g−1 after 100 cycles; 595.9 mA h g−1 @ 5 C rate) were successfully achieved in the anode application of a lithium-ion battery.

    更新日期:2018-01-17
  • Cerium ion intercalated MnO2 nanospheres with high catalytic activity toward oxygen reduction reaction for aluminum-air batteries
    Electrochim. Acta (IF 4.798) Pub Date : 2018-01-17
    Shanshan Sun, Yejian Xue, Qin Wang, Heran Huang, He Miao, Zhaoping Liu

    Manganese oxide is one of the most extensively studied electrocatalysts for oxygen reduction reaction (ORR) due to its high abundance and environmental friendliness. In this work, cerium ion intercalated birnessite-type manganese oxide (δ-MnO2) dispersed on carbon (Ce single bond MnO2/C) with high-efficient oxygen reduction reaction(ORR) electrocatalytic ability in alkaline media is prepared via facile aqueous reactions for the first time. The as-prepared catalyst shows morphology like quasi special nanospheres intertwined by plenty of nanorods just like knitting wool balls. Compared with MnO2/C, the onset and half-wave potential of 4.8% Ce single bond MnO2/C shift positively 27 and 57 mV, respectively, and are close to those of Pt/C. The oxygen reduction reaction occurring on 4.8% Ce single bond MnO2/C undergoes a 4-electron transfer pathway with the HO2− yield lee than 2%, which is much lower than that produced on Pt/C. The Ce single bond MnO2/C catalyst also exhibits excellent long-term stability with current retention of 96.4% after aging for 40000 s. The aluminum-air battery applying 4.8% Ce single bond MnO2/C as cathode catalyst gives out the peak power density of 348.8 mW cm−2 in 4 M KOH aqueous solution. After more than 300-h discharging, the voltage degradation of 4.8% Ce single bond MnO2/C is only 2% per 100 h. In all, the excellent performance of the Ce-intercalated MnO2/C demonstrates its enormous potential as ORR catalyst in metal-air batteries.

    更新日期:2018-01-17
  • Combination of copolymer film (PPy-PPyCOOH) and magnetic nanoparticles as an electroactive and biocompatible platform for electrochemical purposes
    Electrochim. Acta (IF 4.798) Pub Date : 2018-01-17
    Edyta Matysiak-Brynda, Izabela Siekiera, Agata Królikowska, Mikolaj Donten, Anna M. Nowicka

    The process of obtaining the composite layer consisting of conductive copolymer, composed of unmodified pyrrole and pyrrole-3-carboxylic acid units (in various molar ratio) in combination with magnetic nanoparticles was investigated using voltammetry, scanning electron microscopy, transmission electron microscopy, scanning transmission electron microscopy and surface enhanced Raman scattering (SERS) spectroscopy. The SERS and voltammetric data demonstrate that the use of molar concentration ratio of monomers 75 (Py) to 25 mM (Py-COOH) results in the conductive polymer layer with the best bioelectrochemical properties. The ferritin attached covalently to such composite demonstrated the highest electroactivity. Thus, mixed conductive copolymer film containing Py and Py-COOH units, combined with magnetic nanoparticles could be further useful in developing bioactive conductive platforms for specific biomedical purposes.

    更新日期:2018-01-17
  • Hollow spherical lithium-rich layered oxide cathode material with suppressed voltage fading
    Electrochim. Acta (IF 4.798) Pub Date : 2018-01-16
    Weixiang Ding, Xueyang Cui, Jie Lei, Xiaodong Lin, Shengliang Zhao, Qi-Hui Wu, Mingsen Zheng, Quanfeng Dong
    更新日期:2018-01-17
  • Predicting Displaceable Water Sites Using Mixed-Solvent Molecular Dynamics
    J. Chem. Inf. Model. (IF 3.76) Pub Date : 2018-01-16
    Sarah E. Graham, Richard D. Smith, Heather A. Carlson
    更新日期:2018-01-17
  • Improved Descriptors for the QSAR Modeling of Peptides and Proteins
    J. Chem. Inf. Model. (IF 3.76) Pub Date : 2018-01-16
    Mark Howard Barley, Nicholas J Turner, Royston Goodacre

    The ability to model the activity of a protein using Quantitative Structure Activity Relationships (QSAR) requires descriptors for the 20 naturally coded amino acids. In this work we show that by modifying some established descriptors we were able to model the activity data of 140 mutants of the enzyme epoxide hydrolase with improved accuracy. These new descriptors (referred to as Physical descriptors) also gave very good results when tested against a series of four dipeptide datasets. The Physical descriptors encode the amino acids using only two orthogonal scales: the first is strongly linked to hydrophillicity/hydrophobicity, and the second to the volume of the amino acid residue. The use of these new amino acid descriptors should result in simpler and more readily interpretable models for the enzyme activity (and potentially other functions of interest; e.g., secondary and tertiary structure) of peptides and proteins.

    更新日期:2018-01-17
  • Engineering Biocompatible Hydrogels from Bicomponent Natural Nanofibers for Anticancer Drug Delivery
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Junfei Xu, Shan Liu, Guangxue Chen, Ting Chen, Tao Song, Jing Wu, Congcan Shi, Minghui He, Junfei Tian
    更新日期:2018-01-17
  • Inhibition of P-Glycoprotein Mediated Efflux in Caco-2 Cells by Phytic Acid
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Lujia Li, Qingxue Fu, Mengxin Xia, Lei Xin, Hongyi Shen, Guowen Li, Guang Ji, Qianchao Meng, Yan Xie
    更新日期:2018-01-17
  • Roasted barley extract affects blood flow in the rat tail and increases cutaneous blood flow and skin temperature in humans
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-17
    Hiroshi Ashigai, Yoshimasa Taniguchi, Yasuko Matsukura, Emiko Ikeshima, Keiko Nakashima, Mai Mizutani, Hiroaki Yajima

    Roasted barley extract (RBE, “Mugicha”) is a traditional Japanese beverage reported to improve blood viscosity and affect food functionality. RBE is suggested to contain 2,5-diketopiperazines, which are the functional component with neuroprotective and immunostimulatory effects that are produced in food through roasting. In this study, we investigated the effects of RBE on blood circulation, both clinically and in rats. At first, we confirmed five 2,5-diketopiperazine derivatives in RBE by LC-MS analysis. Secondary, we revealed that RBE affects blood flow in the rat tail, and compared the efficacy on rat tail blood flow among five 2,5-diketopiperazine in RBE. Especially, cyclo (D-Phe-L-Pro) was the most effective in increasing blood flow in the rat tail. We also researched the mechanism of cyclo (D-Phe-L-Pro) with rat aorta study. As results, we confirmed that cyclo (D-Phe-L-Pro) has effect on vasodilatation through the release of nitric oxide in the vascular endothelium. Finally, we also confirmed that RBE affects cutaneous blood flow and increases skin temperature in humans.

    更新日期:2018-01-17
  • Unprecedented synergistic effects of nanoscale nutrients on growth, productivity of sweet sorghum (Sorghum bicolor (L.) Moench) and nutrient biofortification
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Naseeruddin Ramapuram, Sumathi V, NVKV Prasad Tollamadugu, p Sudhakar, Chandrika V, Balam Ravindra Reddy

    Evidence based synergistic effects of nanoscale materials (size <100 nm in at least one dimension) were scantly documented in agriculture at field scale. Herein, we report for the first time on effects of nanoscale zinc oxide (n-ZnO), calcium oxide (n-CaO) and magnesium oxide (n-MgO) on growth and productivity of sweet sorghum (Sorghum bicolor (L.) Moench). Modified sol-gel method was used to prepare nanoscale materials under study. Characterization was done using Electron microscopy (TEM & SEM), X-ray diffraction (XRD) and dynamic light scattering (DLS). Average size (25 nm, 53.7 nm, 53.5 nm) and zeta potentials (-10.9mV, -28.2mV, -16.2 mV) of n-ZnO, n-CaO and n-MgO were measured respectively. Significant grain yield (17.8%, 14.2%), cane yield (7.2%, 8.0%), juice yield (10, 12%) and higher sucrose yield (21.8, 20.9%) were recorded with the application of nanoscale materials in the years 2014 and 2015 respectively. Nutrient uptake was significant with foliar application of nanoscale nutrients.

    更新日期:2018-01-17
  • Detection of the Cytotoxic Penitrems A-F in Cheese from the European Single Market (EU) by HPLC-MS/MS
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Svetlana Kalinina, Annika Jagels, Sebastian Hickert, Lucas Maciel Mauriz Marques, Benedikt Cramer, Hans-Ulrich Humpf

    Penitrems are fungal indole diterpene derived tremorgenic secondary metabolites, which are mainly produced by Penicillium spp. Several cases of intoxications with penitrems and subsequent occurrences of penitrem A in foodstuff underline the need for reliable quantitation methods for the detection of these mycotoxins in food. In this study, a simple and fast HPLC-MS/MS method for the quantitative analysis of penitrems A-F in cheese was developed. Therefore, penitrems A-F were isolated from Penicillium crustosum as analytical reference standards. The analysis of 60 cheese samples from the European single market (EU) revealed the occurrence of penitrem A in 10% of the analyzed samples with an average concentration of 28.4 μg/kg and a maximum concentration of 429 μg/kg. In addition to penitrem A, other members of the group of penitrems namely penitrems B, C, D, E, and F, were for the first time quantitatively detected in food samples, although in lower concentrations and with lower incidence in comparison to penitrem A. Moreover, we report cytotoxic effects of all penitrems on two cell lines (HepG2, CCF-STTG1). This clearly underlines their relevance and the importance to analyze food samples in order to get insights into the human exposure towards these mycotoxins.

    更新日期:2018-01-17
  • Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study
    Chem. Biol. Interact. (IF 3.143) Pub Date : 2018-01-17
    Aïcha Sassi, Mouna Maatouk, Dorra El gueder, Imen Mokdad Bzéouich, Saïda Abdelkefi-Ben Hatira, Saloua Jemni-Yacoub, Kamel Ghedira, Leila Chekir-Ghedira
    更新日期:2018-01-17
  • Electrochemical behavior of Polypyrrole/Chitosan composite coating on Ti metal for biomedical applications
    Carbohydr. Polym. (IF 4.811) Pub Date : 2018-01-17
    Bhavana Rikhari, S. Pugal Mani, N. Rajendran

    In the present work, the corrosion resistance performance and biocompatibility of Polypyrrole/Chitosan (PPy/CHI) composite coated Ti was studied. The deposition of composite coating was carried out by electropolymerization method. The deposited PPy/CHI composite coatings were different in morphology, structural, surface roughness and wettability compared PPy coated Ti. The presence of composite coating was confirmed by solid 13C NMR. The PPy/CHI composite coating showed enhanced microhardness and adhesion strength compared to the PPy coating. The corrosion protection ability of PPy/CHI composite coatings at various applied potentials was analyzed by dynamic electrochemical impedance spectroscopy (DEIS), exhibited higher impedance in all the potentials compared to uncoated and PPy coated Ti. The lower corrosion current density obtained for PPy/CHI-2 composite coating from polarization studies revealed increased corrosion protection ability in SBF solution. The stability of composite coating was confirmed by immersion studies. PPy/CHI-2 composite coating immersed in SBF solution enhances hydroxyapatite (HAp) formation.

    更新日期:2018-01-17
  • Fucosylated chondroitin sulfate is covalently associated with collagen fibrils in sea cucumber Apostichopus japonicus body wall
    Carbohydr. Polym. (IF 4.811) Pub Date : 2018-01-16
    Jun Wang, Yaoguang Chang, Fanxiu Wu, Xiaoqi Xu, Changhu Xue

    Fucosylated chondroitin sulfate (fCS) is the major carbohydrate constituent of sea cucumber. However, the distribution of fCS in the sea cucumber body wall has not been fully described. We addressed this in the present study employing Apostichopus japonicus as the material, a sea cucumber species with significant commercial importance. It was found that fCS was covalently attached to collagen fibrils via O-glycosidic linkages. Transmission electron microscopy analysis revealed that fCS precipitate was present in gap regions of collagen fibrils as roughly globular or ellipsoidal dots. The fCS dots arranged circumferentially around the fibrils with an axial repeat period that matched the periodicity of the fibrils. Physicochemical analysis indicated that the presence of fCS significantly increased the negative charge of the fibrils. These findings provide novel insight into fCS distribution in the sea cucumber body wall and its supramolecular organization with other macromolecules.

    更新日期:2018-01-17
  • Effect of retention rate of fluorescent cellulose nanofibrils on paper properties and structure
    Carbohydr. Polym. (IF 4.811) Pub Date : 2018-01-16
    Qijun Ding, Jinsong Zeng, Bin Wang, Wenhua Gao, Kefu Chen, Zhe Yuan, Jun Xu, Darong Tang

    In this work, we report a new characterization method using fluorescent cellulose nanofibrils to analyze retention and loss rates in the papermaking process. Cellulose nanofibrils (CNF) were isolated from chemical pulp by enzymatic pretreatment and used as a strengthening additive in sheet forming. The aim of this paper was to investigate its effects on flocculation, retention and loss rate and the physical and mechanical properties. CNF was subjected to fluorescent labeling with RBITC (Rhodamine B isothiocyanate), and the retention of fluorescent cellulose nanofibrils (FCNF) was analyzed by elemental analysis and fluorescence intensity. The retention and loss rate of the FCNF decreased with increasing the addition of FCNF. Laser Confocal Scanning Microscopy (LCSM) and Scanning Electron Microscope (SEM) images confirmed that FCNF can be evenly distributed in the paper. A thorough investigation of the relation between the retention rate and papersheet performance was conducted.

    更新日期:2018-01-17
  • Dipeptidyl peptidase IV, which probably plays important roles in Alzheimer disease (AD) pathology, is upregulated in AD brain neurons and associates with amyloid plaques
    Neurochem. Int. (IF 3.262) Pub Date : 2018-01-17
    Hans-Gert Bernstein, Henrik Dobrowolny, Gerburg Keilhoff, Johann Steiner

    There is evidence from in vitro experiments that dipeptidyl peptidase IV (DPP IV) might play role(s) in amyloid formation. However, nothing is known about the localization of the enzyme in brains of individuals with Alzheimer's disease. We herein show that in comparison to non-demented controls DPP IV is upregulated in AD brain neurons and occurs in multiple amyloid plaques.

    更新日期:2018-01-17
  • Nicotine alleviates chronic stress-induced anxiety and depressive-like behavior and hippocampal neuropathology via regulating autophagy signaling
    Neurochem. Int. (IF 3.262) Pub Date : 2018-01-16
    Xi Xiao, Xueliang Shang, Baohui Zhai, Hui Zhang, Tao Zhang

    Recently, we reported that chronic nicotine significantly improved chronic stress-induced impairments of cognition and the hippocampal synaptic plasticity in mice, however, the underlying mechanism still needs to be explored. In the present study, 32 male C57BL/6 mice were divided into four groups: control (CON), stress (CUS), stress with chronic nicotine administration (CUS + Nic) and chronic nicotine administration (Nic). The anxiety-like behavior and neuropathological alteration of DG neurons were examined. Moreover, PC12 cells were examined with corticosterone in the presence or absence of nicotine. Both cell viability and apoptosis were determined. When treated simultaneously with an unpredictable chronic mild stress (CUS), nicotine (0.2 mg/kg/d) attenuated behavioral deficits and neuropathological alterations of DG neurons. Moreover, Western blotting showed that chronic nicotine also elevated the level of autophagy makers including Beclin-1 and LC3 II triggered by CUS. In addition, concomitant treatment with nicotine (10 μM) significantly attenuated the loss of PC12 cell viability (p < .01) and apoptosis compared to that of corticosterone treatment alone. Besides, chronic nicotine also enhanced the protein and RNA expression levels of autophagy makers triggered by corticosterone, such as Beclin-1, LC3 II and p62/SQSTM1. However, the above improvements were significantly blocked by autophagy inhibitor 3-MA. Importantly, the activation of the PI3K/Akt/mTOR signaling was carefully tested to illuminate the effects of chronic nicotine. Consequently, chronic nicotine played a role of neuroprotection in either CUS mice or corticosterone cells associating with the enhancement of the autophagy signaling, which was involved in activating the PI3K/Akt/mTOR signaling.

    更新日期:2018-01-17
  • Curcumin potentiates the function of human α7-nicotinic acetylcholine receptors expressed in SH-EP1 cells
    Neurochem. Int. (IF 3.262) Pub Date : 2018-01-16
    Eslam El Nebrisi, Lina T. Al Kury, Keun-Hang Susan Yang, Petrilla Jayaprakash, Frank C. Howarth, Nadine Kabbani, Murat Oz

    Effects of curcumin, a biologically active ingredient of turmeric, were tested on the Ca2+ transients induced by the activation of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor expressed in SH-EP1 cells. Curcumin caused a significant potentiation of choline (1 mM)-induced Ca2+ transients with an EC50 value of 133 nM. The potentiating effect of curcumin was not observed in Ca2+ transients induced by high K+ (60 mM) containing solutions or activation of α4β2 nACh receptors and the extent of curcumin potentiation was not altered in the presence of Ca2+ channel antagonists nifedipine (1 μM), verapamil (1 μM), ω-conotoxin (1 μM), and bepridil (10 μM). Noticeably the effect of curcumin was not observed when curcumin and choline were co-applied without curcumin pre-incubation. The effect of curcumin on choline-induced Ca2+ transients was not reversed by pre-incubation with inhibitors of protein C, A, and CaM kinases. Metabolites of curcumin such as tetrahydrocurcumin, demethylcurcumin, and didemethylcurcumin also caused potentiation of choline-induced Ca2+ transients. Notably, specific binding of [125I]-bungarotoxin was not altered in the presence of curcumin. Collectively, our results indicate that curcumin allosterically potentiate the function of the α7-nACh receptor expressed in SH-EP1 cells.

    更新日期:2018-01-17
  • Formulation and characterization of water-in-oil nanoemulsions loaded with açaí berry anthocyanins: Insights of degradation kinetics and stability evaluation of anthocyanins and nanoemulsions
    Food Res. Int. (IF 3.086) Pub Date : 2018-01-17
    Cezar A.S. Rabelo, Noamane Taarji, Nauman Khalid, Isao Kobayashi, Mitsutoshi Nakajima, Marcos A. Neves
    更新日期:2018-01-17
  • Resveratrol Influences Pancreatic Islets by Opposing Effects on Electrical Activity and Insulin Release
    Mol. Nutr. Food Res. (IF 4.323) Pub Date : 2018-01-17
    Simone Brouwer, Theresa Hoffmeister, Anne Gresch, Lisa Schönhoff, Martina Düfer

    Abstract Scope Resveratrol is suggested to improve glycemic control by activation of sirtuin 1 (SIRT1) and has already been tested clinically. Our investigation characterizes the targets of resveratrol in pancreatic beta cells and their contribution to short- and long-term effects on insulin secretion. Methods and results Islets or beta cells were isolated from C57Bl/6N mice. Electrophysiology was performed with microelectrode arrays and patch-clamp technique, insulin secretion and content were determined by radioimmunoassay, cAMP was measured by enzyme-linked immunosorbent assay and cytosolic Ca2+ concentration by fluorescence methods. Resveratrol (25 μmol/L) elevated [Ca2+]c and potentiated glucose-stimulated insulin secretion. These effects were associated with increased intracellular cAMP and were sensitive to the SIRT1 blocker Ex-527. Inhibition of EPAC1 by CE3F4 also abolished the stimulatory effect of resveratrol. The underlying mechanism did not involve membrane depolarization as resveratrol even reduced electrical activity despite blocking KATP channels. Importantly, after prolonged exposure to resveratrol (14 days), the beneficial influence of the polyphenol on insulin release was lost. Conclusion Resveratrol addresses multiple targets in pancreatic islets. Potentiation of insulin secretion is mediated by SIRT1-dependent activation of cAMP/EPAC1. Considering resveratrol as therapeutic supplement for patients with type 2 diabetes mellitus, the inhibitory influence on electrical excitability attenuates positive effects. This article is protected by copyright. All rights reserved

    更新日期:2018-01-17
  • Stachyose Improves Inflammation Through Modulating Gut Microbiota of High-Fat Diet / Streptozotocin Induced Type 2 Diabetes in Rats
    Mol. Nutr. Food Res. (IF 4.323) Pub Date : 2018-01-16
    Guimei Liu, Jia Bei, Li Liang, Guoyong Yu, Lu Li, Quanhong Li

    Abstract Scope The present study was undertaken to assess the effects of stachyose (STS) on type 2 diabetes in rats, and changes in the gut microbiota compared to metformin (MET). Methods and results The type 2 diabetic model was successfully established via a high-fat diet (HFD) / streptozotocin (STZ) in Wistar rats, and STS or MET was administered for 4 weeks. Blood was collected to analyze biochemical parameters, pancreas for mRNA expression of related gene and contents of colon for gut microbiota. STS or MET decreased serum lipopolysaccharide (LPS), mRNA expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). In addition, STS and MET showed a similar shifting of the structure of the gut microbiota, and a selective enrichment of key species. At the genus level, STS showed selective enrichment of Phascolarctobacterium, Bilophila, Oscillospira, Turicibacter and SMB5, but MET demonstrated a selective effect on Sutterella, Prevotella, 02d06 and rc4. The correlation analysis indicated that STS and MET decrease IL-6 and TNF-α and increase Akt/PI3K expression which were relative to key species of gut microbiota. Conclusion STS decreased pancreatic mRNA expression of IL-6 and TNF-α via key species of gut microbiota. The mechanism of this effect was similar to that of MET. This article is protected by copyright. All rights reserved

    更新日期:2018-01-17
  • Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides
    Food Res. Int. (IF 3.086) Pub Date : 2018-01-16
    Pádraigín A. Harnedy, Vadivel Parthsarathy, Chris M. McLaughlin, Martina B. O'Keeffe, Philip J. Allsopp, Emeir M. McSorley, Finbarr P.M. O'Harte, Richard J. FitzGerald

    Large quantities of low-value protein rich co-products, such as salmon skin and trimmings, are generated annually. These co-products can be upgraded to high-value functional ingredients. The aim of this study was to assess the antidiabetic potential of salmon skin gelatin and trimming-derived protein hydrolysates in vitro. The gelatin hydrolysate generated with Alcalase 2.4 L and Flavourzyme 500 L exhibited significantly higher (p < .001) insulin and GLP-1 secretory activity from pancreatic BRIN-BD11 and enteroendocrine GLUTag cells, respectively, when tested at 2.5 mg/mL compared to hydrolysates generated with Alcalase 2.4 L or Promod 144MG. The gelatin hydrolysate generated with Alcalase 2.4 L and Flavourzyme 500 L showed significantly more potent (p < .01) DPP-IV inhibitory activity than those generated with Alcalase 2.4 L or Promod 144MG. No significant difference was observed in the insulinotropic activity mediated by any of the trimming-derived hydrolysates when tested at 2.5 mg/mL. However, the trimmings hydrolysate generated with Alcalase 2.4 L and Flavourzyme 500 L exhibited significantly higher DPP-IV inhibitory (p < .05:Alcalase 2.4 L and p < .01:Promod 144MG) and GLP-1 (p < .001, 2.5 mg/mL) secretory activity than those generated with Alcalase 2.4 L or Promod 144MG. The salmon trimmings hydrolysate generated with Alcalase 2.4 L and Flavourzyme 500 L when subjected to simulated gastrointestinal digestion (SGID) was shown to retain its GLP-1 secretory and DPP-IV inhibitory activities, in addition to improving its insulin secretory activity. However, the gelatin hydrolysate generated with Alcalase 2.4 L and Flavourzyme 500 L was shown to lose GLP-1 secretory activity following SGID. A significant increase in membrane potential (p < .001) and intracellular calcium (p < .001) by both co-product hydrolysates generated with Alcalase 2.4 L and Flavourzyme 500 L suggest that both hydrolysates mediate their insulinotropic activity through the KATP channel-dependent pathway. Additionally, by stimulating a significant increase in intracellular cAMP release (p < .05) it is likely that the trimming-derived hydrolysate may also mediate insulin secretion through the protein kinase A pathway. The results presented herein demonstrate that salmon co-product hydrolysates exhibit promising in vitro antidiabetic activity.

    更新日期:2018-01-17
  • C24-hydroxylated stigmastane derivatives as Liver X Receptor agonists
    Chem. Phys. Lipids (IF 3.361) Pub Date : 2018-01-17
    Francisco Fermin Castro Navas, Gianluca Giorgi, Daniela Maggioni, Manuela Pacciarini, Vincenzo Russo, Maura Marinozzi

    Phytosterols are stucturally correlated to the endogenous ligands of Liver X Receptor (LXR), a ligand-activated nuclear receptor that has emerged as an attractive drug target due to its ability to integrate metabolic and inflammatory signaling. Natural and semi-synthetic phytosterol derivatives characterized by the presence of side-chain oxygenated functions have shown to be able to modulate LXR activity. Here, we describe the efficient synthesis of four stigmastane derivatives, endowed with a hydroxyl group at C24 position, namely (24R)- and (24S)-stigmasta-5,28-diene-3β,24-ols (also referred to as saringosterols, 10a and 10b) and (24R)- and (24S)-stigmasta-5-ene-3β,24-ols (11a and 11b), starting from the readily available stigmasterol. Thanks to X-ray crystallography the absolute configuration of the newly created chiral centers was definitively assigned for all the four compounds. The subsequent luciferase assays with GAL-4 chimeric receptors evidenced the ability of the two 24(S)-epimers, 10b and 11b, to interact with LXRs, showing the same degree of affinity as (22R)-hydroxycholesterol (1). With regard to the isoform selectivity both the derivatives 10b and 11b showed a preference for LXRβ, up to 4-fold in terms of efficacy for 11b. The gene expression profiling of (24S)-stigmasta-5,28-diene-3β,24-ol (10a) and (24S)-stigmasta-5-ene-3β,24-ol (11a) demonstrated the capability of both the compounds to induce the expression of four well-known LXR target genes, such as ABCA1, SREBP1c, FASN, and SCD1 in U937 monocytic cell line, thus supporting the hypothesis they were LXR positive modulators.

    更新日期:2018-01-17
  • Coproducts performances in biorefineries: Development of Claiming-Based Allocation models for Environmental policy
    Bioresource Technol. (IF 5.651) Pub Date : 2018-01-16
    Edgard Gnansounou

    This study revisited the fundamentals of allocation to joint products and proposed new models for allocating common greenhouse gases emissions among coproducts of biorefineries. These emissions may account for more than 80% of the total emissions of greenhouse gases of the biorefineries. The proposed models optimize the reward of coproducts for their compliance to environmental requirements. They were illustrated by a case study of wheat straw biorefinery built on the literature. Several scenarios were considered with regard to the grain yield, field emissions of greenhouse gases, allocation between grain and straw and policy requirements. The results conform to the expectations and are sensitive to the policy targets and to the environmental performance of the counterpart system. Further research works are necessary to achieve a full application to complex processes. However, the proposed models are promising towards assessing the simultaneous compliance of coproducts of a biorefinery to environment policy requirements.

    更新日期:2018-01-17
  • Screening of a microbial consortium for selective degradation of lignin from tree trimmings
    Bioresource Technol. (IF 5.651) Pub Date : 2018-01-16
    Xiuxiu Fang, Qiumin Li, Yunqin Lin, Xinlei Lin, Yiqi Dai, Zexiang Guo, Dezhao Pan
    更新日期:2018-01-17
  • TEMPORAL ACCLIMATION OF MICROCHLOROPSIS GADITANA CCMP526 IN RESPONSE TO HYPERSALINITY
    Bioresource Technol. (IF 5.651) Pub Date : 2018-01-16
    Anbarasu Karthikaichamy, Pranali Deore, Sanjeeva Srivastava, Ross Coppel, Dieter Bulach, John Beardall, Santosh Noronha

    Evaporation from culture ponds and raceways can subject algae to hypersalinity stress, and this is exacerbated by global warming. We investigated the effect of salinity on a marine microalga, Microchloropsis gaditana, which is of industrial significance because of its high lipid-accumulating capability. Both short-term (hours) and medium-term (days) effects of salinity were studied across various salinities (37.5, 55, 70 and 100 PSU). Salinity above 55 PSU supressed cell growth and specific growth rate was significantly reduced at 100 PSU. Photosynthesis (Fv/Fm, rETRmax and Ik) was severely affected at high salinity conditions. Total carbohydrate per cell increased ∼1.7 fold after 24 hr, which is consistent with previous findings that salinity induces osmolyte production to counter osmotic shock. In addition, accumulation of lipid increased by ∼4.6 fold in response to salinity. Our findings indicate a possible mechanism of acclimation to salinity, opening up new frontiers for osmolytes in pharmacological and cosmetics applications.

    更新日期:2018-01-17
  • Dam construction impacts on multiscale characterization of sediment discharge in two typical karst watersheds of southwest China
    J. Hydrol. (IF 3.483) Pub Date : 2018-01-16
    Zhenwei Li, Xianli Xu, Chaohao Xu, Meixian Liu, Kelin Wang
    更新日期:2018-01-17
  • Optimal Spatio-temporal Design of Water Quality Monitoring Networks for Reservoirs: Application of the Concept of Value of Information
    J. Hydrol. (IF 3.483) Pub Date : 2018-01-16
    Nahal Maymandi, Reza Kerachian, Mohammad Reza Nikoo

    This paper presents a new methodology for optimizing WQM networks of reservoirs and lakes using the concept of the value of information (VOI) and utilizing results of a calibrated numerical water quality simulation model. With reference to the value of information theory, water quality of every checkpoint with a specific prior probability differs in time. After analyzing water quality samples taken from potential monitoring points, the posterior probabilities are updated using the Baye's theorem, and VOI of the samples is calculated. In the next step, the stations with maximum VOI is selected as optimal stations. This Process is repeated for each sampling interval to obtain optimal monitoring network locations for each interval. The results of the proposed VOI-based methodology is compared with those obtained using an entropy theoretic approach. As the results of the two methodologies would be partially different, in the next step, the results are combined using a weighting method. Finally, the optimal sampling interval and location of WQM stations are chosen using the Evidential Reasoning (ER) decision making method. The efficiency and applicability of the methodology are evaluated using available water quantity and quality data of the Karkheh Reservoir in the southwestern part of Iran.

    更新日期:2018-01-17
  • Updating techniques on controlling mycotoxins - A review
    Food Control (IF 3.496) Pub Date : 2018-01-16
    Ying Luo, Xiaojiao Liu, Jianke Li

    Mycotoxins are secondary metabolites produced by fungal species and have been reported to be carcinogenic, genotoxic, teratogenic, nephro- and hepatotoxic. Economic losses and health concerns due to mycotoxins occur at all levels of food and feed production, and this has attracted research interest towards exploring novel approaches to decontaminate and/or detoxify mycotoxin-contaminated food and feed. Therefore, we reviewed various strategies for the detoxification of mycotoxins, including pre-harvest prevention strategies and post-harvest detoxification procedures. Strategies for pre-harvest prevention include appropriate environmental factors, good agricultural and manufacturing practices, and favourable storage practices. For post-harvest detoxification strategies, physical, chemical, biological and other developing innovative strategies are discussed. This review is meant to be beneficial to the food industry and to contribute to assuring pre- and post-harvest management and processing practices that maximize consumer safety.

    更新日期:2018-01-17
  • Occurrence and co-occurrence of mycotoxins in nuts and dried fruits from China
    Food Control (IF 3.496) Pub Date : 2018-01-16
    Yujiao Wang, Jiyun Nie, Zhen Yan, Zhixia Li, Yang Cheng, Weixia Chang

    In this study, 16 mycotoxins were analyzed in ten kinds of dried fruits and nuts sampled from four climate zones in China. The results showed that all 16 mycotoxins were detected at a contamination frequency of 124/253. The most frequent mycotoxin category detected was TCs with contamination levels ranging from

    更新日期:2018-01-17
  • Listeria monocytogenes at chicken slaughterhouse: occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility
    Food Control (IF 3.496) Pub Date : 2018-01-16
    Tainara Santos Oliveira, Luana Milen Varjão, Lis Nery Nunes da Silva, Rodrigo de Castro Lisboa Pereira, Ernesto Hofer, Deyse Cristina Vallim, Rogeria Comastri de Castro Almeida

    The aims of this study were to verify the occurrence of Listeria monocytogenes in chicken at industrial slaughterhouse, the presence of virulence genes and genetic relationship among isolates, and evaluate the antimicrobial susceptibility. A total of 195 samples distributed in 36 carcasses after evisceration, 36 after chilling and 36 after packing, 43 carcasses cutting and 44 cuts after packaging, were collected. L. monocytogenes was found in 35 (17.9%) samples, especially in meat cuts, and samples collected after packing were the ones with higher rate of contamination (8.3%). Serotyping revealed the presence of three serovars: 1/2a (87%), 1/2c (8%) and 1/2b (5%). Macrorestriction profile with Apal at Pulsed-field gel electrophoresis (PFGE) showed 12 pulsotypes, and isolates from carcass and drumettes after packing and breast filet from cuts section showed high pulsotypes diversity. Regarding the profile of antimicrobial susceptibility, 100% of the isolates were sensitive to most antibiotics tested, except for clindamycin; 42% had intermediate resistance and 5% resistance. Isolation of L. monocytogenes in high levels in the chicken slaughtering line, as found in this study points to a potential risk associated, because the pathogen remains viable in the food throughout the cold chain that is used to commercialization. Still, the antimicrobial susceptibility monitoring is of interest of public health and our result shows that the isolates were susceptible to almost all antibiotics tested, indicating that treatment commonly used to control listeriosis infection is adequate.

    更新日期:2018-01-17
  • 7-Hydroxylation of warfarin is strongly inhibited by sesamin, but not by episesamin, caffeic and ferulic acids in human hepatic microsomes
    Food Chem. Toxicol. (IF 3.778) Pub Date : 2018-01-17
    Nadezhda Pilipenko, Martin Krøyer Rasmussen, Olena Doran, Galia Zamaratskaia

    Warfarin is a commonly used anticoagulant drug and is a derivate of coumarin. Cytochrome P450 2C9 (CYP2C9) plays the key role in transformation of coumarin and thus, influences determination of warfarin dosage. A number of factors including dietary compounds such as sesamin, caffeic acid and ferulic acids can regulate the activity of CYP2C9. The present study tested the hypothesis that sesamin, episesamin, caffeic acid and ferulic acid decreases the rate of warfarin 7-hydroxylation via inhibition of hepatic CYP2C9. The experiments were conducted on hepatic microsomes from human donors. It was demonstrated that the rate of 7-hydroxylation of warfarin was significantly decreased in the presence of sesamin in the range of concentrations from 5 to 500 nM, and was not affected by episesamin, caffeic acid and ferulic acid in the same range of concentrations. The kinetic analysis indicated non-competitive type of inhibition by sesamin with Ki = 202 ± 18 nM. In conclusion, the results of our in vitro study revealed that sesamin was able to inhibit formation of a major metabolite of warfarin, 7-hydroxywarfarin. The potentially negative consequences of the consumption of high amounts of sesamincontaining food or dietary supplements in warfarin-treated patients need to be further studied.

    更新日期:2018-01-17
  • Lanthanum chloride impairs memory in rats by disturbing the glutamate-glutamine cycle and over-activating NMDA receptors
    Food Chem. Toxicol. (IF 3.778) Pub Date : 2018-01-17
    Xiaoyu Hu, Jinghua Yang, Yaling Sun, Xiang Gao, Lijin Zhang, Yingqi Li, Miao Yu, Shiyu Liu, Xiaobo Lu, Cuihong Jin, Shengwen Wu, Yuan Cai

    Studies have reported that lanthanum chloride (LaCl3) can across the blood-brain barrier, accumulate in the brain and affect the spatial learning and memory abilities. However, the potential mechanism that LaCl3-induced neurotoxic effects has not yet been defined. Glutamate (Glu) is a vital excitatory neurotransmitter, and the excessive Glu accumulation in extracellular space can induce excitatory neurotoxicity. This study was designed to research the influence of LaCl3 on the spatial learning and memory abilities of rats and to discuss the possible mechanism underlying this effect regarding the extracellular Glu concentration, the Glu-glutamine (Gln) cycle and the N-methyl-D-aspartate (NMDA) receptors. Four groups of Wistar rats were exposed to 0%, 0.125%, 0.25% or 0.5% LaCl3 via the drinking water from the day of conception to 1 month after weaning. These results showed that LaCl3 exposure damaged spatial learning and memory, long-term potentiation, and neuronal ultrastructure, generated an excessive accumulation of glutamate, significantly decreased the expression of glutamate/aspartate transporter (GLAST), glutamate transporter-1 (GLT-1), glutamine synthetase (GS) and phosphate-activated glutaminase (PAG), and increased the expression of GluN1, GluN2A and GluN2B. This study showed that LaCl3 impaired the rats' spatial learning and memory abilities by disturbing the Glu-Gln cycle and over-activating NMDA receptors thereby inducing excitotoxicity.

    更新日期:2018-01-17
  • Effects of warfarin on biological processes other than haemostasis: A review
    Food Chem. Toxicol. (IF 3.778) Pub Date : 2018-01-17
    Aleksandra Popov Aleksandrov, Ivana Mirkov, Marina Ninkov, Dina Mileusnic, Jelena Demenesku, Vesna Subota, Dragan Kataranovski, Milena Kataranovski

    Warfarin is the world's most widely used anticoagulant drug. Its anticoagulant activity is based on the inhibition of the vitamin K-dependent (VKD) step in the complete synthesis of a number of blood coagulation factors that are required for normal blood coagulation. Warfarin also affects synthesis of VKD proteins not related to haemostasis including those involved in bone growth and vascular calcification. Antithrombotic activity of warfarin is considered responsible for some aspects of its anti-tumour activity of warfarin. Some aspects of activities against tumours seem not to be related to haemostasis and included effects of warfarin on non-haemostatic VKD proteins as well as those not related to VKD proteins. Inflammatory/immunomodulatory effects of warfarin indicate much broader potential of action of this drug both in physiological and pathological processes. This review provides an overview of the published data dealing with the effects of warfarin on biological processes other than haemostasis.

    更新日期:2018-01-17
  • Concentrations of arsenic and lead in rice (Oryza sativa L.) in Iran: A systematic review and carcinogenic risk assessment
    Food Chem. Toxicol. (IF 3.778) Pub Date : 2018-01-16
    Yadolah Fakhri, Geir Bjørklund, Anoushiravan Mohseni Bandpei, Salvatore Chirumbolo, Hassan Keramati, Rokhsane Hosseini Pouya, Anvar Asadi, Nazak Amanidaz, Mansour Sarafraz, Amir Sheikhmohammadi, Zahra Baninameh, Seyed Mohsen Mohseni, Maryam Sarkhosh, Seyed Mehdi Ghasemi
    更新日期:2018-01-17
  • Ternary Composite Nanosheets with MoS2/WS2/Graphene Heterostructures as High Performance Cathode Materials for Supercapacitors
    ChemElectroChem (IF 4.136) Pub Date : 2018-01-15
    Tsung-Wu Lin, Thangarasu Sadhasivam, Ai-Yin Wang, Ting-Yu Chen, Jeng-Yu Lin, Li-Dong Shao

    A new class of ternary heterostructure consisting of a reduced graphene oxide, molybdenum disulfide and tungsten disulfide (rGO-MoS2-WS2) is prepared through a simple chemical method. According to Raman and X-ray photoelectron spectroscopies, MoS2/WS2 heterostructure is uniformly formed on the conductive rGO support. Furthermore, rGO-MoS2-WS2 possesses the large surface area of 109 m2 g-1 and the hierarchical pore architecture. When served as the electrode for supercapacitors, rGO-MoS2-WS2 exhibits the pseudocapacitive behavior in a KOH solution. It is found that rGO-MoS2-WS2 displays the specific capacitance (Cs) of 365 F g-1 at 1 A g-1 being much higher than those of single TMD (MoS2 or WS2) based composites. The enhanced electrochemical performance of rGO-MoS2-WS2 is achieved due to the lower internal resistance through heterostructure formation, the wide range of TMD oxidation states and uniform distribution of the few-layered TMD nanosheets on the rGO surface. Furthermore, the optimal loading amount of MoS2/WS2 heterostructure in the composite is investigated. To demonstrate its practical application, rGO-MoS2-WS2 is used as a positive electrode for an asymmetric supercapacitor (ASC). The maximum energy density of the ASC device is 15 Wh kg-1 at the power density of 373 W kg-1. Furthermore, the device remains ca. 70 % of the initial Cs value after 3000 cycles, which shows the excellent cycling stability.

    更新日期:2018-01-16
  • Improvement of Current Efficiency in Anodic Chlorination of Poly(3-hexylthiophene) by using a Boron Trifluoride-Diethyl Ether Complex
    ChemElectroChem (IF 4.136) Pub Date : 2018-01-16
    Tomoyuki Kurioka, Hiroki Nishiyama, Ikuyoshi Tomita, Shinsuke Inagi
    更新日期:2018-01-16
  • Stability and Antioxidant Activity of Annatto (Bixa orellana L.) Tocotrienols During Frying and in Fried Tortilla Chips
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Jill K. Winkler-Moser, Erica L. Bakota, Hong-Sik Hwang

    Abstract Annatto tocotrienols (AnT3), which contain approximately 90% δ-tocotrienol (δ-T3), were added to mid-oleic sunflower oil used for frying tortilla chips over 3 d. The objectives were to evaluate their stability during frying, absorption by the fried food, and activity as antioxidants in frying oil and in tortilla chips during storage. AnT3 did not significantly affect the stability of the oil during frying or the sensory profiles of freshly fried chips. The naturally present α-tocopherol (α-T) in the oil degraded at a lower rate in the presence of AnT3, resulting in significantly higher α-T by the end of the frying study. Levels of tocopherols and tocotrienols in the chips mirrored oil levels. AnT3 did not affect the sensory profile of the chips after 1 wk of storage at 50 °C, but after 3 wk of storage, the control chips had higher levels of painty and rancid flavors compared to chips with AnT3. Headspace hexanal was also significantly higher in the control chips compared to the chips with AnT3 after 3 wk of storage. Practical Application Annatto tocotrienols, containing primarily delta- and gamma-tocotrienols, were added to mid-oleic sunflower oil used for frying tortilla chips. The tocotrienols were absorbed by the chips along with the oil. They slowed the degradation of α tocopherol during frying, and reduced levels of painty and rancid flavor scores as well as headspace hexanal in chips that were stored for 3 wk at elevated temperatures. The results indicated that fried snack foods such as tortilla chips may be a suitable and convenient vehicle for enriching tocotrienols in the diet, and that tocotrienols may also enhance the shelf-life of fried foods.

    更新日期:2018-01-16
  • Selection of Yeast Strains for Tequila Fermentation Based on Growth Dynamics in Combined Fructose and Ethanol Media
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    J. A. Aldrete-Tapia, D. E. Miranda-Castilleja, S. M. Arvizu-Medrano, M. Hernández-Iturriaga

    Abstract The high concentration of fructose in agave juice has been associated with reduced ethanol tolerance of commercial yeasts used for tequila production and low fermentation yields. The selection of autochthonous strains, which are better adapted to agave juice, could improve the process. In this study, a 2-step selection process of yeasts isolated from spontaneous fermentations for tequila production was carried out based on analysis of the growth dynamics in combined conditions of high fructose and ethanol. First, yeast isolates (605) were screened to identify strains tolerant to high fructose (20%) and to ethanol (10%), yielding 89 isolates able to grow in both conditions. From the 89 isolates, the growth curves under 8 treatments of combined fructose (from 20% to 5%) and ethanol (from 0% to 10%) were obtained, and the kinetic parameters were analyzed with principal component analysis and k-means clustering. The resulting yeast strain groups corresponded to the fast, medium and slow growers. A second clustering of only the fast growers led to the selection of 3 Saccharomyces strains (199, 230, 231) that were able to grow rapidly in 4 out of the 8 conditions evaluated. This methodology differentiated strains phenotypically and could be further used for strain selection in other processes. Practical Application A method to select yeast strains for fermentation taking into account the natural differences of yeast isolates. This methodology is based on the cell exposition to combinations of sugar and ethanol, which are the most important stress factors in fermentation. This strategy will help to identify the most tolerant strain that could improve ethanol yield and reduce fermentation time.

    更新日期:2018-01-16
  • Phytochemicals, Anti-Inflammatory, Antiproliferative, and Methylglyoxal Trapping Properties of Zijuan Tea
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Xiong Gao, Chi-Tang Ho, Xiaofei Li, Xiaorong Lin, Yuanyuan Zhang, Zhongzheng Chen, Bin Li

    Abstract Zijuan tea (Camellia sinensis var. assamica) is a unique anthocyanin-rich tea cultivar in China. Although chemical component analysis of Zijuan tea and extraction technology of anthocyanins was widely documented, its functional properties have not been extensively explored. In this study, the anti-inflammatory, antiproliferative, and methylglyoxal (MGO) trapping activities of water extract (ZWE) and ethyl acetate extract (ZEE) of Zijuan tea were investigated for the 1st time. Results showed that ZWE and ZEE exhibited inhibitory effects on nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 production as well as inducible nitric oxide synthase protein (iNOS) expression in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Moreover, Zijuan tea extracts exerted stronger antiproliferative activity against HCT-116 cells compared with HepG2 and MDA-MB-231 cells, and thus could induce apoptosis in HCT-116 cells in a dose-dependent manner. Furthermore, Zijuan tea extracts were effective in trapping MGO under simulated physiological conditions, and the T1/2 (the time for 50% MGO remaining) values of ZWE and ZEE were 3.69 and 6.20 min, respectively. Additionally, the contents of total phenolics and catechins in ZEE were 685.43 ± 16.00 and 454.96 ± 4.21 mg/g extract, respectively, and in ZWE were 422.59 ± 12.09 and 307.29 ± 0.85 mg/g extract, respectively. Therefore, ZEE exhibited better anti-inflammatory, antiproliferative, and MGO trapping properties than ZWE may be mainly attributed to its higher (P < 0.05) content of total phenolics, expecially catechins. These results suggest that Zijuan tea could be a potential natural resource for the development of functional tea beverage. Practical Application This study revealed that Zijuan tea extracts possessed anti-inflammatory, antiproliferative, and methylglyoxal trapping potentials in vitro. With high anthocyanins and polyphenols, Zijuan tea can be developed into a healthy tea beverage or used as a natural component to reduce the level of methylglyoxal in Maillard reaction.

    更新日期:2018-01-16
  • Antimicrobial-Resistance Genetic Markers in Potentially Pathogenic Gram Positive Cocci Isolated from Brazilian Soft Cheese
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Juliana Alves Resende, Cláudia Oliveira Fontes, Alessandra Barbosa Ferreira-Machado, Thiago César Nascimento, Vânia Lúcia Silva, Cláudio Galuppo Diniz

    Abstract Although most Brazilian dairy products meet high technological standards, there are quality issues regarding milk production, which may reduce the final product quality. Several microbial species may contaminate milk during manufacture and handling. If antimicrobial usage remains uncontrolled in dairy cattle, the horizontal transfer of antimicrobial resistance genes in foodstuffs may be of particular concern for both food producers and dairy industry. This study focused on the evaluation of putative Gram positive cocci in Minas cheese and of antimicrobial and biocide resistance genes among the isolated bacteria. Representative samples of 7 different industrially trademarked Minas cheeses (n = 35) were processed for selective culture and isolation of Gram positive cocci. All isolated bacteria were identified by DNA sequencing of the 16S rRNA gene. Antimicrobial resistance genes were screened by PCR. Overall, 208 strains were isolated and identified as follows: Enterococcus faecalis (47.6%), Macrococcus caseolyticus (18.3%), Enterococcus faecium (11.5%), Enterococcus caseliflavus (7.7%), Staphylococcus haemolyticus (7.2%), Staphylococcus aureus (4.3%), Staphylococcus epidermidis (2.9%), and Enterococcus hirae (0.5%). The genetic markers mecA (78.0%) and smr (71.4%) were the most prevalent, but others were also detected, such as blaZ (65.2%), msrA (60.9%), msrB (46.6%), linA (54.7%), and aacA-aphD (47.6%). The occurrence of opportunist pathogenic bacteria harboring antimicrobial resistance markers in the cheese samples are of special concern, since these bacteria are not considered harmful contaminating agents according to the Brazilian sanitary regulations. However, they are potentially pathogenic bacteria and the cheese may be considered a reservoir for antimicrobial resistance genes available for horizontal transfer through the food chain, manufacturing personnel and consumers.

    更新日期:2018-01-16
  • Hepatoprotective Effects of the Honey of Apis cerana Fabricius on Bromobenzene-Induced Liver Damage in Mice
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Haoan Zhao, Ni Cheng, Liangliang He, Guoxia Peng, Qingqing Liu, Tianchen Ma, Wei Cao

    Abstract Apis cerana honey (honey of Apis cerana Fabricius), widely distributed in the mountain areas of East Asia, has not been studied fully. The hepatoprotective activity of A. cerana honey was evaluated against bromobenzene-induced liver damage in mice. In high dose, A. cerana honey can significantly alleviate liver injury, as is indicated by the depressed levels of serum alanine aminotransferase (ALT) (59.13%) and aspartate aminotransferase (AST) (79.71%), the inhibited malondialdehyde (MDA) content (63.30%), the elevated activities of superoxide dismutase (SOD) (73.12%) and glutathione-Px (57.24%), and the decreased expression of Transforming growth factor β1 (51.83%) induced by bromobenzene (P < 0.05). The quantitative analysis of twelve major constituents (1 to 12) of A. cerana honey was executed by high performance liquid chromatography-diode array detector. The results indicate that treatment with A. cerana honey can prevent bromobenzene-induced hepatic damage in mice. Polyphenols might be the bioactive substances attributed to its antioxidant properties and intervention of oxidative stress.

    更新日期:2018-01-16
  • Polysaccharides Reduce Absorption and Mutagenicity of 3-Amino-1,4-Dimethyl-5H-Pyrido[4,3-b]Indole In Vitro and In Vivo
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Lingying Luo, Yun Bai, Guanghong Zhou

    Abstract 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) is a group 2B carcinogen characterized by the International Agency for Research on Cancer (IARC) and great efforts have been taken to reduce Trp-P-1 mutagenicity to humans. In this study, the effect of a reduction of Trp-P-1 on intestinal absorption as a promising strategy was investigated. The data showed that when 20 mM Trp-P-1 cotransported with 10 mM of sodium carboxymethyl cellulose (CMC), xanthan gum, or carrageenan, the absorption rate of Trp-P-1 was reduced by 31.5%, 49.5%, or 72.9% in MDCK-MDR1 cell monolayer, respectively; and 64.6%, 83.4%, or 64.1% in rat intestinal tissues, correspondingly. These 3 polysaccharides also reduced pharmacokinetic parameters, that is, Cmax, AUC0-t, and AUC0-∞, after Trp-P-1 was given to rats intragastrically. However, gum arabic did not exhibit similar effects on Trp-P-1 absorption in vitro or in vivo. The Ames test showed that these 3 polysaccharides reduced Trp-P-1 mutagenicity to Salmonella typhimurium TA98, but gum arabic did not. Isothermal titration calorimetry assay indicated that Trp-P-1 interacted with these 3 polysaccharides. Thermodynamic study showed that the actual value of △H <0, but its absolute value greater than the corresponding value of T∆S, suggest a specific interaction between Trp-P-1 and these 3 polysaccharides, probably through the hydrogen bond and/or ion interaction. Reduction of Trp-P-1 intestinal absorption using food additives could be one of the strategies to suppress Trp-P-1-induced carcinogenesis in human. Practical Application 1. This study provides insightful information for the food industry how gum arabic, xanthan gum, kappa carrageenan, and sodium carboxymethyl cellulose affect the absorption of Trp-P-1. 2. This study also provides novel information regarding a better formulation for meat products to reduce Trp-P-1 absorption.

    更新日期:2018-01-16
  • Moisture Adsorption Isotherm and Storability of Hazelnut Inshells and Kernels Produced in Oregon, USA
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Jooyeoun Jung, Wenjie Wang, Robert J. McGorrin, Yanyun Zhao

    Abstract Moisture adsorption isotherms and storability of dried hazelnut inshells and kernels produced in Oregon were evaluated and compared among cultivars, including Barcelona, Yamhill, and Jefferson. Experimental moisture adsorption data fitted to Guggenheim–Anderson–de Boer (GAB) model, showing less hygroscopic properties in Yamhill than other cultivars of inshells and kernels due to lower content of carbohydrate and protein, but higher content of fat. The safe levels of moisture content (MC, dry basis) of dried inshells and kernels for reaching kernel water activity (aw) ≤0.65 were estimated using the GAB model as 11.3% and 5.0% for Barcelona, 9.4% and 4.2% for Yamhill, and 10.7% and 4.9% for Jefferson, respectively. Storage conditions (2 °C at 85% to 95% relative humidity [RH], 10 °C at 65% to 75% RH, and 27 °C at 35% to 45% RH), times (0, 4, 8, or 12 mo), and packaging methods (atmosphere vs. vacuum) affected MC, aw, bioactive compounds, lipid oxidation, and enzyme activity of dried hazelnut inshells or kernels. For inshells packaged at woven polypropylene bag, MC and aw of inshells and kernels (inside shells) increased at 2 and 10 °C, but decreased at 27 °C during storage. For kernels, lipid oxidation and polyphenol oxidase activity also increased with extended storage time (P < 0.05), and MC and aw of vacuum packaged samples were more stable during storage than those atmospherically packaged ones. Principal component analysis showed correlation of kernel qualities with storage condition, time, and packaging method. This study demonstrated that the ideal storage condition or packaging method varied among cultivars due to their different moisture adsorption and physicochemical and enzymatic stability during storage. Practical Application Moisture adsorption isotherm of hazelnut inshells and kernels is useful for predicting the storability of nuts. This study found that water adsorption and storability varied among the different cultivars of nuts, in which Yamhill was less hygroscopic than Barcelona and Jefferson, thus more stable during storage. For ensuring food safety and quality of nuts during storage, each cultivar of kernels should be dried to a certain level of MC. Lipid oxidation and enzyme activity of kernel could be increased with extended storage time. Vacuum packaging was recommended to kernels for reducing moisture adsorption during storage.

    更新日期:2018-01-16
  • Replenishment of Docosahexaenoic Acid (DHA) in Dietary n-3-Deficient Mice Fed DHA in Triglycerides or Phosphatidylcholines after Weaning
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Dan-dan Wang, Fang Wu, Min Wen, Lin Ding, Lei Du, Chang-hu Xue, Jie Xu, Yu-ming Wang

    Abstract Previous studies have shown that DHA in triglyceride (TG) and phosphatidylcholine (PC) forms are different in their bioavailability. The aim of this study was to investigate the comparative effects of DHA-TG and DHA-PC on tissue DHA accretion in dietary n-3 polyunsaturated fatty acid deficient (n-3 Def) mice. The mice were fed with n-3 Def diet containing DHA-TG or DHA-PC (5 g/kg diet) for 2, 4, 7, or 14 d after weaning, respectively. The DHA levels in the cortex, liver, testis, and erythrocytes were analyzed by gas chromatography. For liver, DHA mainly existed in hepatic phospholipids relative to triglycerides. Both DHA-TG and DHA-PC could recover the hepatic DHA to a normal level. Interestingly, DHA-TG was more effective in increasing the DHA level in hepatic triglycerides, and DHA-PC was more effective in increasing the DHA level in hepatic phospholipids. For erythrocytes, during the first 7 d, no difference was observed after dietary DHA-TG and DHA-PC but a significantly higher DHA percentage was detected in the DHA-PC group after 14 d. For cortex, the DHA-TG group showed a higher cortical DHA level at the 4th day, but the DHA-PC group showed a higher cortical DHA level with a greater slope from Day 7 to Day 14, and the same trend was observed in testis. But unexpectedly, the DHA level in testis showed a downtrend from Day 7 to Day 14. This study suggests that, under dietary n-3-deficient condition, both DHA-TG and DHA-PC could recover the DHA level in tissues after weaning, and DHA-PC showed a better supplemental effect. Practical Application Dietary DHA is essential for neurodevelopment which is usually accompanied by large amounts of DHA accretion in the brain. Our present study showed that DHA-PC had a better efficiency for DHA accretion in the brain and other tissues compared with DHA-TG. The findings are supposed to pave the way for the DHA in phospholipids as a novel nutrient added into the infant formula and assisted food for neurodevelopment.

    更新日期:2018-01-16
  • Microbiological Quality and Safety of Fresh Fruits and Vegetables at Retail Levels in Korea
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Charles Nkufi Tango, Shuai Wei, Imran Khan, Mohammad Shakhawat Hussain, Paul-François Ngnitcho Kounkeu, Joong-hyun Park, Se-hun Kim, Deog Hwan Oh

    Abstract The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed. Presence of pathogens was examined using real-time polymerase chain reaction (q-PCR) after enrichment of samples. For all, the microbial counts ranged from 1.7 to 10.6 log cfu/g for AMB, 2.2 to 7.9 log cfu/g for coliforms, and 5.5 to 7.9 log cfu/g for YM. Three lettuce samples were contaminated by E. coli with a bacterial load ranging from 2 to 4 log cfu/g. Salmonella spp. were not detected in any fresh produce. Listeria monocytogenes, E. coli O157:H7, and Staphylococcus aureus were found in 1 (0.6%), 3 (0.8%), and 5 (1.4%) fresh produce samples, respectively. Bacillus cereus (50.3%) and Clostridium perfringens (13.3%) had the highest prevalence. These results indicate the need for employing strict control measures and developing preventive strategies to improve the quality and safety of fresh produce in Korea.

    更新日期:2018-01-16
  • Consumer Acceptance Comparison Between Seasoned and Unseasoned Vegetables
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Yiming Feng, Marta Albiol Tapia, Kyle Okada, Nuria Blanca Castaneda Lazo, Karen Chapman-Novakofski, Carter Phillips, Soo-Yeun Lee

    Abstract Recent findings show that approximately 87% of the U.S. population fail to meet the vegetable intake recommendations, with unpleasant taste of vegetables being listed as the primary reason for this shortfall. In this study, spice and herb seasoning was used to enhance palatability of vegetables, in order to increase consumer acceptance. In total, 749 panelists were screened and recruited as specific vegetable likers of the vegetable being tested or general vegetable likers. Four sessions were designed to evaluate the effect of seasoning within each type of vegetable, including broccoli, cauliflower, carrot, and green bean. Each panelist was only allowed to participate in one test session to evaluate only one vegetable type, so as to mitigate potential learning effect. Overall, the results showed that seasoned vegetables were significantly preferred over unseasoned vegetables (P < 0.001), indicating the sensory properties were significantly improved with seasoning. When general vegetable likers and specific vegetable likers were compared in terms of their preference between seasoned and unseasoned vegetables, the pattern varied across different vegetables; however, general trend of seasoned vegetable being preferred remained. The findings from this study demonstrate the effect of seasoning in enhancing consumer liking of vegetables, which may lead to increased consumption to be assessed in future studies. Practical Application To improve the sensory properties of vegetables, masking the bitter taste of vegetables using spice and herb seasoning are gaining increasing attention. Our findings suggest that the overall liking of vegetables could be improved by incorporating spice and herb seasonings that are specifically formulated for each vegetable. Ultimately, developing and commercializing spice and herb seasonings may aid to increase vegetable consumption, as well as expanding the vegetable seasoning market.

    更新日期:2018-01-16
  • The Function of Emulsions on the Biogenic Amine Formation and their Indices of Sea Bass Fillets (Dicentrarchus Labrax) Stored in Vacuum Packaging
    J. Food Sci. (IF 1.815) Pub Date : 2018-01-16
    Yesim Ozogul, Mustafa Durmus, Esmeray Kuley Boga, Yılmaz Uçar, Fatih Ozogul

    Abstract The impacts of emulsions based on commercial oils on the biogenic amine formation and their indices of vacuumed packed sea bass fillets were investigated. The results showed that among biogenic amines, cadaverine, putrescine, spermidine, spermine, serotonin, dopamine, and agmatine were predominant amines in sea bass fillets stored under vacuum packaging. Significant differences (P < 0.05) in biogenic amines concentrations of vacuumed packed sea bass treated with emulsions were observed. All groups contained histamine lower than 5.0 mg/100 g, regarded as the allowable limit by the U.S. Food and Drug Administration. Polyamine levels were not affected by application of emulsion. Quality index (QI) showed an increase and after 14 d of storage it decreased in all groups. The control generally seemed to higher QI value than those of treatment groups except at 14 and 18 days while soybean and corn gave lower QI among treatment groups. Only biogenic amine index correlated with sensory acceptability of vacuumed packed sea bass, indicating that this index can be used for determination of the degree of spoilage of vacuumed packed sea bass. Emulsions extended the shelf-life (approximately 2 to 4 d) of vacuumed packed sea bass fillets by inhibiting microbial growth compared to the control. Practical Application Emulsions have become popular since they are regarded as ideal carrier for the delivery of lipophilic substances due to the ease of preparation, small particle size, their enhanced bioavailability, and long term kinetic stability. They have been proven to be self-preserving antimicrobials due to bound water in their structure and thus no available water to microorganisms. Antimicrobial emulsions have potential applications in many fields because they are inexpensive, stable, and nontoxic agents.

    更新日期:2018-01-16
  • Human Intestinal Dendritic Cells in Inflammatory Bowel Diseases
    Mol. Nutr. Food Res. (IF 4.323) Pub Date : 2018-01-16
    David Bernardo, María Chaparro, Javier P Gisbert

    Abstract Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is a serious, costly and persistent health problem with an estimated prevalence in Western countries around 0.5% of the general population; its socioeconomic impact is comparable with that for chronic diseases such as diabetes. Conventional treatment involves escalating drug regimens with concomitant side effects followed, in some cases, by surgical interventions which are often multiple, mainly in Crohn's disease. The goal of finding a targeted gut-specific immunotherapy for IBD patients is therefore an important unmet need. However, to achieve this goal we first must understand how dendritic cells (DC), the most potent antigen present cells of the immune system, control the immune tolerance in the gastrointestinal tract and how their properties are altered in those patients suffering from IBD. In this review, we summarize therefore the current available information regarding human intestinal DC subsets composition, phenotype and function in the human gastrointestinal tract describing how, in the IBD mucosa, DC display pro-inflammatory properties which drive disease progression. A better understanding of the mechanisms inducing DC abnormal profile in IBD may provide us therefore with novel tools to perform tissue specific immunomodulation. This article is protected by copyright. All rights reserved

    更新日期:2018-01-16
  • Human Milk Oligosaccharides as Promising Antivirals
    Mol. Nutr. Food Res. (IF 4.323) Pub Date : 2018-01-16
    Vasily Morozov, Grant Hansman, Franz-Georg Hanisch, Horst Schroten, Clemens Kunz

    Abstract Human milk oligosaccharides (HMOs) are diverse unconjugated carbohydrates that are highly abundant in human breast milk. These glycans are investigated in the context of exhibiting multiple functions in infant growth and development. They seem to provide protection against infectious diseases, including a number of poorly manageable viral infections. Although the potential mechanism of the HMO antiviral protection is rather broad, much of the current experimental work has focused on studying of HMO antiadhesive properties. HMOs may mimic structures of viral receptors and block adherence to target cells, thus preventing infection. Still, the potential of HMOs as a source for new antiviral drugs is relatively unexploited. This can be partly attributed to the extreme complexity of the virus-carbohydrate interactions and technical difficulties in HMO isolation, characterization and manufacturing procedures. Fortunately, we are currently entering a period of major technological advances that have enabled deeper insights into carbohydrate mediated viral entry, rational selection of HMOs as anti-entry inhibitors, and even evaluation of individual synthetic HMO structures. Here, we provide an up-to-date review on glycan binding studies for rotaviruses, noroviruses, influenza viruses, and human immunodeficiency viruses. We also discuss the preventive and therapeutic potential of HMOs as anti-entry inhibitors and address challenges on the route from fundamental studies to clinical trials. This article is protected by copyright. All rights reserved

    更新日期:2018-01-16
  • Resveratrol Potently Counteracts Quercetin Starvation-Induced Autophagy and Sensitizes HepG2 Cancer Cells to Apoptosis
    Mol. Nutr. Food Res. (IF 4.323) Pub Date : 2018-01-16
    Sarah Tomas-Hernández, Jordi Blanco, Cristina Rojas, Joel Roca-Martínez, María José Ojeda-Montes, Raúl Beltrán-Debón, Santiago Garcia-Vallve, Gerard Pujadas, Lluís Arola, Miquel Mulero

    Abstract Scope Resveratrol (RSV) has been described as a potent antioxidant, anti-steatotic, and antitumor compound, and it has also been identified as a potent autophagy inducer. On the other hand, quercetin (QCT) is a dietary flavonoid with known antitumor, anti-inflammatory and antidiabetic effects. Additionally, QCT increases autophagy. To study the hypothetical synergistic effect of both compounds, we tested the combined effect of QCT and RSV on the autophagy process in HepG2 cells Methods and results Autophagy was studied by western blotting, real-time RT-PCR, and cellular staining. Our results clearly indicate a bifunctional molecular effect of RSV. Both polyphenols were individually able to promote autophagy. Strikingly, when RSV was combined with QCT, it promoted a potent reduction of QCT-induced autophagy and influenced pro-apoptotic signaling. Conclusion RSV acts differentially on the autophagic process depending on the cellular energetic state. We further characterized the molecular mechanisms related to this effect, and we observed that AMP-activated protein kinase (AMPK) phosphorylation, heme oxygenase 1 (HO-1) downregulation, lysosomal membrane permeabilization (LMP) and Zinc (Zn2+) dynamics could be important modulators of such RSV-related effects and could globally represent a promising strategy to sensitize cancer cells to QCT treatment. This article is protected by copyright. All rights reserved

    更新日期:2018-01-16
  • Urolithins Attenuate LPS-Induced Neuroinflammation in BV2Microglia via MAPK, Akt, and NF-κB Signaling Pathways
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Jialin Xu, Chunhui Yuan, Guihua Wang, Jiaming Luo, Hang Ma, Li Xu, Yu Mu, Yuanyuan Li, Navindra P. Seeram, Xueshi Huang, Liya Li
    更新日期:2018-01-16
  • 更新日期:2018-01-16
  • Synthesis of (Hexaconazole-Zinc/Aluminum-Layered Double Hydroxide Nanocomposite) Fungicide Nanodelivery System for Controlling Ganoderma Disease in Oil Palm
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Isshadiba F. Mustafa, Mohd Zobir Hussein, Bullo Saifullah, Abu Seman Idris, Nur Hailini Z. Hilmi, Sharida Fakurazi
    更新日期:2018-01-16
  • Uptake and Accumulation of Pharmaceuticals in Overhead- and Surface-Irrigated Greenhouse Lettuce
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-01-16
    Gemini D. Bhalsod, Ya-Hui Chuang, Sangho Jeon, Wenjun Gui, Hui Li, Elliot T. Ryser, Andrey K. Guber, Wei Zhang
    更新日期:2018-01-16
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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