Selenium (Se) and sulfur (S) speciation analysis in edible and medicinal Se enriched P. pulmonarius extracts was performed. Mycelium, colonized substrate, and fruiting bodies at different harvesting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS detector. Extraction efficiencies in enzymatically digested and aqueous extracts were between 45.3 and 109% for Se, depending on the sample type. Selenomethionine (Se-Met) was found to be the major Se-compound, together with a number of unknown Se-species. Cystine (Cys₂), methionine (Met), and sulfate were also detected and quantified in all samples. Most of the Se-Met (84.0%) and Met (75.8%) were found to be in free form in the fruiting body, in contrast with the mycelium where 53.4% of Se-Met and 80.5% of Met is incorporated into proteins.

在食用和药用富硒肺炎球菌提取物中进行了硒（Se）和硫（S）形态分析。使用结合ICPMS / MS检测器的离子对反相色谱法分析了不同收获时间的菌丝体，定殖的底物和子实体。酶消化和水提取物中硒的提取效率在45.3％至109％之间，具体取决于样品类型。发现硒代蛋氨酸（Se-Met）是主要的硒化合物，还有许多未知的硒物种。胱氨酸（Cys ₂），蛋氨酸（Met）和硫酸盐也都在所有样品中进行了检测和定量。发现子实体中的大多数Se-Met（84.0％）和Met（75.8％）是游离形式，而菌丝体中53.4％的Se-Met和80.5％的Met被掺入蛋白质中。