With in-vitro digestion and human intestinal HepG2 cells, we analyzed the bioaccessibility and cell uptake of phytochemicals and determined the cellular antioxidant capacity (CAA) of fresh eating citrus fruits. The results showed that CAA of citrus fruits was higher in digesta than in extracts, and the CAA is strongly correlated with naringenin and beta-carotene uptake (p < 0.05). During in vitro digestion, vanillic acid and p-coumaric decreased, and ferulic acid increased in all citrus fruits significantly (p < 0.05); other phytochemicals varied among the fruits. During uptake, hydroxybenzoic acids, hesperidin, narirutin, naringenin and neohesperidin were detected in cells, Zeaxanthin, lutein, beta-cryptoxanthin and beta-carotene could be detected in the citrus varieties except for pummel, but hydroxycinnamic acids and hesperitin were not detected in cells. This work provides insights into the bioaccessibility and cell uptake of phytochemicals and cellular antioxidant activity of fresh eating citrus fruits.

通过体外消化和人类肠道HepG2细胞，我们分析了植物化学物质的生物可及性和细胞摄取，并确定了新鲜食用柑橘类水果的细胞抗氧化能力（CAA）。结果表明，柑桔类水果的CAA在消化液中的含量高于提取物中的含量，并且CAA与柚皮素和β-胡萝卜素的吸收密切相关（p  <0.05）。在体外所有柑橘类水果的消化，香草酸和对香豆酸含量均降低，而阿魏酸含量则显着升高（p <0.05）；水果中的其他植物化学物质也有所不同。在摄取过程中，在细胞中检出了羟基苯甲酸，橙皮苷，纳瑞汀，柚皮苷和新橙皮苷，除柚子外，在柑橘品种中还检出了玉米黄质，叶黄素，β-隐黄质和β-胡萝卜素，但在细胞中未检出羟基肉桂酸和橙皮素。 。这项工作提供了对新鲜食用柑橘类水果植物化学物质的生物可及性和细胞吸收以及细胞抗氧化活性的见解。