Interleukin 8 is known as an important chemokine and plays an important role in tumor growth and angiogenesis. Over expression of IL-8 has been detected in a variety of human tumors, including gastric cancer and breast cancer. Therefore, the development of rapid and sensitive profiling methods is crucial for evaluating the level of IL-8 expression in human serum, which is related to normal and tumor states. In our study, surface-enhanced Raman scattering (SERS) based on the double antibody sandwich format is reported for the determination of IL-8. Highly-branched gold nanoparticle (HGNP) substrates were fabricated and conjugated with anti-IL-8 (coating) as capturing substrates, and gold nanocages (GNCs) adsorbed with 4-mercaptobenzoic acid are modified with anti-IL-8 (labeling) as SERS tags. After the substrates captured IL-8, the SERS tags were bonded to the captured IL-8. The interaction of the SERS capturing substrates (HGNPs) and the SERS tags (GNCs) shows high sensitivity and a low detection limit for IL-8. The linear dynamic range of SERS for IL-8 was from 10 pg mL⁻¹ to 1 μg mL⁻¹, which shows a linear relationship between IL-8 concentration and SERS intensity. The detection limit was 6.04 pg mL⁻¹ in phosphate buffered saline and 6.88 pg mL⁻¹ in human serum. The analysis results of serum samples obtained from healthy subjects and two different types of cancer patients (breast cancer and gastric cancer) by using the SERS method were acceptably consistent with those obtained by the enzyme-linked immunosorbent assay. The proposed immunoassay shows great potential for application in clinical diagnosis.

中文翻译：

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表面增强拉曼散射结合高支化金纳米颗粒和金纳米笼检测人血清中的IL-8

白介素8被称为重要的趋化因子，在肿瘤生长和血管生成中起着重要作用。已经在包括胃癌和乳腺癌的多种人类肿瘤中检测到IL-8的过度表达。因此，快速和敏感的分析方法的发展对于评估人血清中IL-8表达水平至关重要，而IL-8表达水平与正常和肿瘤状态有关。在我们的研究中，据报道基于双抗体夹心格式的表面增强拉曼散射（SERS）用于测定IL-8。制备了高度支化的金纳米颗粒（HGNP）底物，并与抗IL-8（涂层）结合作为捕获底物，并用抗IL-8（标记）对吸附有4-巯基苯甲酸的金纳米笼（GNC）进行了修饰，如下所示： SERS标签。底物捕获IL-8后，SERS标签与捕获的IL-8结合。SERS捕获底物（HGNP）与SERS标签（GNC）的相互作用显示出对IL-8的高灵敏度和低检测限。IL-8的SERS线性动态范围为10 pg mL^-1至1μgmL ^-1，其在IL-8浓度和SERS强度之间呈线性关系。在磷酸盐缓冲液中的检出限为6.04 pg mL ^-1，在人血清中的检出限为6.88 pg mL ^-1。使用SERS方法从健康受试者和两种不同类型的癌症患者（乳腺癌和胃癌）中获得的血清样品的分析结果与酶联免疫吸附法获得的结果相吻合。所提出的免疫测定法在临床诊断中显示出巨大的潜力。