Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.

中文翻译：

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蛋白质复合物的多色单颗粒重建

通过电子显微镜（EM）图像进行的单粒子重建（SPR）已广泛用于结构生物学，但缺乏有关蛋白质同一性的直接信息。为了解决这一局限性，我们开发了一种计算和分析框架，该框架可从2D超分辨率荧光图像重建并共对齐多种蛋白质。为了证明我们的方法，我们在人类中心体中生成了几种蛋白质的多色3D重建体，揭示了它们的相对位置，尺寸和方向。