Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.

中文翻译：

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CRISPR-Sirius：用于基因组成像中信号放大的 RNA 支架。

成簇的规则间隔短回文重复序列 (CRISPR) 引导 RNA 支架已被改造为携带荧光蛋白的多个结合位点，以增强基因组位点活细胞成像的亮度。然而，其中许多修饰会导致指导 RNA 不稳定，从而产生比预期更低的基因组标记效率。在这里，我们介绍了基于八位组核酸适体阵列的 CRISPR-Sirius，增强了向导 RNA 的稳定性和亮度，并提供了该平台的初步生物学应用。