Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).

中文翻译：

---

用于验证抗体的高通量管线

Western blotting（WB）被广泛用于测试抗体的特异性，但该方法的通量和精确度较低。在这里，我们使用了制备性凝胶电泳来开发WB的捕获格式。含有可溶的，大小分离的蛋白质的馏分有助于通过抗体阵列，shot弹枪质谱（MS）和免疫沉淀后再进行MS（IP-MS）的并行读取。该管道为国际上抗体验证工作组（IWGAV）提出的抗体验证概念的大规模实施提供了手段。