Abstract Networks of electron beam irradiated polyaniline (Irr-PANi) supported with bovine serum albumin (BSA) biocomposite is employed as electrochemical biosensor for simultaneous determination of epinephrine (EP) and uric acid (UA). Facilitated electron transfer was associated with modified biocomposite, as a result an enhancement in electro oxidation of EP and UA occurs. In Irr-PANi/BSA biocomposite, rich pore channels, conformational bahavior and redox mediator role of BSA outperformed the catalytic activity to detect EP and UA well suited for wide range of detection 500 nM–700 μM and 300 nM–800 μM with detection limits 26 and 16 nM (S/N = 3σ/b) respectively. The biofunctionalized composite has demonstrated highly sensitive, selective and reproducible electrochemical sensing for EP and UA. These characteristics of the biocomposite are extremely suitable to resolve EP and UA in black tea and chicken samples with satisfactory results.

中文翻译：

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电子射线辐照聚苯胺锚定在牛血清白蛋白上，用于同时检测肾上腺素和尿酸

摘要 采用牛血清白蛋白 (BSA) 生物复合材料支持的电子束辐照聚苯胺 (Irr-PANi) 网络作为电化学生物传感器同时测定肾上腺素 (EP) 和尿酸 (UA)。促进电子转移与改性生物复合材料有关，因此增强了 EP 和 UA 的电氧化。在 Irr-PANi/BSA 生物复合材料中，BSA 的丰富孔道、构象行为和氧化还原介体作用优于催化活性来检测 EP 和 UA，非常适用于 500 nM–700 μM 和 300 nM–800 μM 的广泛检测范围，具有检测限分别为 26 和 16 nM (S/N = 3σ/b)。生物功能化复合材料已证明对 EP 和 UA 具有高度灵敏、选择性和可重复的电化学传感。