The aim of this study is to prepare the long linear aliphatic amine pendant group-functionalized chitosan-based nanoparticulate gene carrier system with improved properties for the efficient transfection. The amine functionalized chitosan (MChi) was synthesized by using N-(2-hydroxyethyl)ethylenediamine (HE-EDA) and characterized for the first time. The nanoparticles of MChi (nMChi) were prepared by ionic gelation method, and their particle size, polydispersity (PDI), zeta potential (mV), gene binding capacity and cytotoxicity were determined. Green Fluorescent Protein circular plasmid DNA (pEGFN1) loaded nanoparticles (gnMChi) were used in the transfection studies. The results showed that nMChi with a particle size of 102.9 nm and zeta potential of 41.9 ± 5.63 mV was non-toxic, had high transfection efficiency in Human Embryonic Kidney 293 and Primary Ovine Fibroblast cell lines and would be used as an efficient gene carrier system.

这项研究的目的是制备具有改进特性的长线性脂肪族胺侧基官能化壳聚糖基纳米颗粒基因载体系统，以提高有效转染的性能。使用N-（2-羟乙基）乙二胺（HE-EDA）合成了胺官能化的壳聚糖（MChi），并进行了首次表征。通过离子凝胶法制备了MChi的纳米颗粒（nMChi），并对其粒径，多分散性（PDI），ζ电势（mV），基因结合能力和细胞毒性进行了测定。加载绿色荧光蛋白的环状质粒DNA（pEGFN1）纳米颗粒（gnMChi）用于转染研究。结果表明，粒径为102.9 nm，ζ电位为41.9±5.63 mV的nMChi是无毒的，